################################## # NO CUT ENZYMES ( 220 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmFI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeI MaeIII MamI MboII MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 27 enzymes) # ################################### AciI 6 GCGG CCGC(-3/-1) AluI 192 AGCT AG^CT AlwI 15 GGATC GGATC(4/5) AvaI 123 CCCGAG C^[CT]CG[GA]G BsaAI 49 CACGTA [CT]AC^GT[GA] BsaJI 124 CCGAGG C^C[ACGT][ACGT]GG BsiYI 202 CCAAGAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 202 CCAAGAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmBI 30 GAGACG CGTCTC(1/5) BsmI 184 GCATTC GAATGC(1/-1) BsoBI 123 CCCGAG C^[CT]CG[GA]G BsrBI 4 GAGCGG CCGCTC(-3/-3) BssKI 165 CCAGG ^CC[ACGT]GG BstNI 165 CCAGG CC^[AT]GG DpnII 16 GATC ^GATC EcoRII 165 CCAGG ^CC[AT]GG FauI 6 GCGGG CCCGC(4/6) MaeII 50 ACGT A^CGT MboI 16 GATC ^GATC MvaI 165 CCAGG CC^[AT]GG NdeII 16 GATC ^GATC PflMI 202 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Sau3AI 16 GATC ^GATC ScrFI 165 CCAGG CC^[ACGT]GG SfcI 38 CTACAG C^T[GA][CT]AG TspRI 95 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 202 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 7 enzymes) # ################################### BbvI 138 GCAGC GCAGC(8/12) 141 GCAGC BsmAI 30 GAGAC GTCTC(1/5) 196 GAGAC Cac8I 6 GCGGGC GC[ACGT]^[ACGT]GC 180 GCAGGC Fnu4HI 138 GCAGC GC^[ACGT]GC 141 GCAGC ItaI 138 GCAGC GC^[ACGT]GC 141 GCAGC MwoI 129 GCGTTCTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 184 GCATTCTTAGC TseI 138 GCAGC G^C[AT]GC 141 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 4 enzymes) # ######################################### AfaI DdeI MnlI RsaI