################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccI AccII AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaI BsgI BsiHKAI BsiWI BsmAI BsmBI BsmI BspDI BspHI BspLU11I BspMI BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraII DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FbaI FseI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MaeII MfeI MluI MluNI MscI MslI MspA1I MunI MvnI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SphI Sse8387I StuI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccIII 407 TCCGGA T^CCGGA AcyI 141 GGCGTC G[GA]^CG[CT]C Asp700I 991 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 30 TGCGCA TGC^GCA BanI 231 GGTGCC G^G[CT][GA]CC BanII 43 GAGCCC G[GA]GC[CT]^C BmyI 43 GAGCCC G[AGT]GC[ACT]^C BsaBI 220 GATATAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 141 GGCGTC G[GA]^CG[CT]C BseAI 407 TCCGGA T^CCGGA BseRI 478 GAGGAG GAGGAG(10/8) BsiEI 119 CGGCCG CG[GA][CT]^CG Bsp1286I 43 GAGCCC G[AGT]GC[ACT]^C BspEI 407 TCCGGA T^CCGGA BsrFI 54 GCCGGC [GA]^CCGG[CT] BsrI 742 ACTGG ACTGG(1/-1) BstXI 251 CCAATCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 54 GCCGGC [GA]^CCGG[CT] DraI 637 TTTAAA TTT^AAA EagI 119 CGGCCG C^GGCCG EarI 109 GAAGAG CTCTTC(1/4) EclXI 119 CGGCCG C^GGCCG Eco52I 119 CGGCCG C^GGCCG EcoRV 949 GATATC GAT^ATC EcoT14I 149 CCATGG C^C[AT][AT]GG FauI 66 CCCGC CCCGC(4/6) FspI 30 TGCGCA TGC^GCA HgaI 142 GCGTC GACGC(5/10) Hin1I 141 GGCGTC G[GA]^CG[CT]C HphI 1117 TCACC GGTGA(8/7) Ksp632I 109 GAAGAG CTCTTC(1/4) MamI 220 GATATAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 407 TCCGGA T^CCGGA NaeI 54 GCCGGC GCC^GGC NcoI 149 CCATGG C^CATGG NgoMIV 54 GCCGGC G^CCGGC NspI 962 ACATGC [GA]CATG^[CT] SpeI 804 ACTAGT A^CTAGT SspI 1061 AATATT AAT^ATT StyI 149 CCATGG C^C[AT][AT]GG TaqI 615 TCGA T^CGA TthHB8I 615 TCGA T^CGA XmnI 991 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AvaI 46 CCCGGG C^[CT]CG[GA]G 359 CCCGGG AvaII 261 GGTCC G^G[AT]CC 669 GGACC BbsI 958 GAAGAC GAAGAC(2/6) 991 GAAGAC BpuAI 958 GAAGAC GAAGAC(2/6) 991 GAAGAC BsaWI 228 TCCGGT [AT]^CCGG[AT] 407 TCCGGA BsmFI 200 GGGAC GGGAC(10/14) 144 GTCCC BsoBI 46 CCCGGG C^[CT]CG[GA]G 359 CCCGGG Bsp1407I 194 TGTACA T^GTACA 1128 TGTACA BsrGI 194 TGTACA T^GTACA 1128 TGTACA BstNI 171 CCTGG CC^[AT]GG 605 CCTGG Cac8I 41 GCGAGC GC[ACGT]^[ACGT]GC 54 GCCGGC CfoI 31 GCGC GCG^C 58 GCGC DdeI 535 CTAAG C^T[ACGT]AG 548 CTCAG EaeI 5 CGGCCA [CT]^GGCC[GA] 119 CGGCCG EcoRII 171 CCTGG ^CC[AT]GG 605 CCTGG HhaI 31 GCGC GCG^C 58 GCGC HinP1I 31 GCGC G^CGC 58 GCGC HinfI 175 GACTC G^A[ACGT]TC 1114 GACTC MvaI 171 CCTGG CC^[AT]GG 605 CCTGG PleI 175 GACTC GAGTC(4/5) 1114 GACTC PstI 319 CTGCAG CTGCA^G 658 CTGCAG SfcI 319 CTGCAG C^T[GA][CT]AG 658 CTGCAG SmaI 46 CCCGGG CCC^GGG 359 CCCGGG SspBI 194 TGTACA T^GTACA 1128 TGTACA XmaCI 46 CCCGGG C^CCGGG 359 CCCGGG XmaI 46 CCCGGG C^CCGGG 359 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BbvI BcnI BfaI BsaJI BsiYI BslI BssKI BstF5I BstYI Cfr13I DpnII DsaI Fnu4HI FokI HaeIII HapII HpaII ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MspI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI Tru9I TseI Tsp45I Tsp509I TspRI XhoII