(Circular) (Six-base) MAPSORT of: pFN21AE1859.seq Check: 5441 from: 1 to: 5842 pFN21AE1859 5842 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3652 278 Size: 53 83 186 936 2116 2468 Fragments arranged by size: 2468 2116 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3185 1937 Size: 1248 4594 Acc65I G'GTAC_C Cuts at: 3163 3163 Size: 5842 AclI AA'CG_TT Cuts at: 1563 3969 4342 1563 Size: 2406 373 3063 Fragments arranged by size: 3063 2406 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2420 3905 5289 5557 1632 Size: 788 1485 1384 268 1917 Fragments arranged by size: 1917 1485 1384 788 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5645 AflIII A'CryG_T Cuts at: 1203 4741 5718 1203 Size: 3538 977 1327 Fragments arranged by size: 3538 1327 977 AgeI A'CCGG_T Cuts at: 4673 4673 Size: 5842 AhdI GACnn_n'nnGTC Cuts at: 4571 4571 Size: 5842 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5842 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5842 AlwNI CAG_nnn'CTG Cuts at: 3585 5157 3585 Size: 1572 4270 ApaLI G'TGCA_C Cuts at: 3899 5055 3899 Size: 1156 4686 ApoI r'AATT_y Cuts at: 1493 2288 2564 2639 3151 3377 3529 5638 1493 Size: 795 276 75 512 226 152 2109 1697 Fragments arranged by size: 2109 1697 795 512 276 226 152 75 AseI AT'TA_AT Cuts at: 160 1032 4396 160 Size: 872 3364 1606 Fragments arranged by size: 3364 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5842 AvaI C'yCGr_G Cuts at: 1870 1943 2051 2364 3167 1870 Size: 73 108 313 803 4545 Fragments arranged by size: 4545 803 313 108 73 BamHI G'GATC_C Cuts at: 3172 3539 3172 Size: 367 5475 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2236 3163 4618 618 Size: 325 217 627 449 927 1455 1842 Fragments arranged by size: 1842 1455 927 627 449 325 217 BanII G_rGCy'C Cuts at: 1391 2052 3161 1391 Size: 661 1109 4072 Fragments arranged by size: 4072 1109 661 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2970 3003 928 Size: 971 1071 33 3767 Fragments arranged by size: 3767 1071 971 33 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4033 4067 1903 Size: 34 2096 34 3678 Fragments arranged by size: 3678 2096 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3734 4944 3734 Size: 1210 4632 BclI T'GATC_A Cuts at: 1548 1548 Size: 5842 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4453 136 Size: 107 122 71 631 605 337 2444 1525 Fragments arranged by size: 2444 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5837 5837 Size: 5842 BlpI GC'TnA_GC Cuts at: 3259 3259 Size: 5842 Bme1580I G_kGCm'C Cuts at: 1790 3903 5059 1790 Size: 2113 1156 2573 Fragments arranged by size: 2573 2113 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5842 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3573 4531 442 Size: 416 53 83 399 24 2156 958 1753 Fragments arranged by size: 2156 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5842 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4502 1239 Size: 3263 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3967 4832 5130 3967 Size: 865 298 4679 Fragments arranged by size: 4679 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4505 882 Size: 617 3006 2219 Fragments arranged by size: 3006 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3633 493 Size: 711 2429 2702 Fragments arranged by size: 2702 2429 711 BsaBI GATnn'nnATC Cuts at: 2229 5836 2229 Size: 3607 2235 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2147 3649 4031 4666 Size: 53 83 186 936 614 1502 382 635 Cuts at: 4666 275 Size: 1451 Fragments arranged by size: 1502 1451 936 635 614 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2233 2412 4275 4673 4947 5094 5505 2233 Size: 179 1863 398 274 147 411 2570 Fragments arranged by size: 2570 1863 411 398 274 179 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2171 2201 1178 Size: 30 165 30 768 30 4819 Fragments arranged by size: 4819 768 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2498 1186 Size: 1312 4530 BseYI C'CCAG_C Cuts at: 2076 2097 2132 5045 5541 2076 Size: 21 35 2913 496 2377 Fragments arranged by size: 2913 2377 496 35 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5269 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2127 3292 4053 5081 1540 Size: 275 186 126 1165 761 1028 2301 Fragments arranged by size: 2301 1165 1028 761 275 186 126 BsiHKAI G_wGCw'C Cuts at: 3903 3988 5059 3903 Size: 85 1071 4686 Fragments arranged by size: 4686 1071 85 BsmI GAATG_Cn' Cuts at: 3353 3446 3353 Size: 93 5749 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 3161 3903 3988 5059 1391 Size: 399 262 1109 742 85 1071 2174 Fragments arranged by size: 2174 1109 1071 742 399 262 85 BspEI T'CCGG_A Cuts at: 2412 5505 2412 Size: 3093 2749 BspHI T'CATG_A Cuts at: 3731 5461 3731 Size: 1730 4112 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3197 844 Size: 2353 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3729 1394 Size: 429 1906 3507 Fragments arranged by size: 3507 1906 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4337 4511 65 Size: 1183 3089 174 1396 Fragments arranged by size: 3089 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2059 4486 4673 1700 Size: 110 249 2427 187 2869 Fragments arranged by size: 2869 2427 249 187 110 BsrGI T'GTAC_A Cuts at: 96 2199 3133 96 Size: 2103 934 2805 Fragments arranged by size: 2805 2103 934 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5842 BssSI C'ACGA_G Cuts at: 3902 4914 3902 Size: 1012 4830 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5842 BstBI TT'CG_AA Cuts at: 4646 4646 Size: 5842 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5842 BstXI CCAn_nnnn'nTGG Cuts at: 2263 2263 Size: 5842 BstYI r'GATC_y Cuts at: 1972 2230 2443 2545 3086 3172 3539 3924 3941 Size: 258 213 102 541 86 367 385 17 Cuts at: 3941 5393 5479 5491 5837 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 541 385 367 346 258 213 102 86 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2142 2154 2262 513 Size: 552 939 138 12 108 4093 Fragments arranged by size: 4093 939 552 138 108 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5103 Fragments arranged by size: 5103 683 56 BtsI GCAGTG_nn' Cuts at: 2469 3358 4152 4172 2469 Size: 889 794 20 4139 Fragments arranged by size: 4139 889 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5842 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5807 DraI TTT'AAA Cuts at: 2644 3146 2644 Size: 502 5340 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4849 809 Size: 507 3533 1802 Fragments arranged by size: 3533 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2124 3289 Size: 54 1425 50 275 18 180 114 1165 Cuts at: 3289 4178 5807 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1165 889 275 180 114 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2124 3289 1537 Size: 275 312 1165 4090 Fragments arranged by size: 4090 1165 312 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2109 3772 1353 Size: 756 1663 3423 Fragments arranged by size: 3423 1663 756 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4429 4803 4949 387 Size: 1064 138 2840 374 146 1280 Fragments arranged by size: 2840 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2420 3905 4502 5289 5557 1239 Size: 393 788 1485 597 787 268 1524 Fragments arranged by size: 1524 1485 788 787 597 393 268 EcoRI G'AATT_C Cuts at: 3151 3529 3151 Size: 378 5464 EcoRV GAT'ATC Cuts at: 2956 2956 Size: 5842 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3020 3020 Size: 5842 FspI TGC'GCA Cuts at: 2037 4348 2037 Size: 2311 3531 HaeII r_GCGC'y Cuts at: 1678 4989 1678 Size: 3311 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2171 2203 4483 4515 4557 Size: 32 135 32 599 32 2280 32 42 Cuts at: 4557 4589 1373 Size: 32 2626 Fragments arranged by size: 2626 2280 599 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3186 3432 669 Size: 988 281 1248 246 3079 Fragments arranged by size: 3079 1248 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5842 HpaI GTT'AAC Cuts at: 3432 3432 Size: 5842 KpnI G_GTAC'C Cuts at: 3167 3167 Size: 5842 MfeI C'AATT_G Cuts at: 3441 3441 Size: 5842 MluI A'CGCG_T Cuts at: 5718 5718 Size: 5842 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4956 5140 1305 Size: 3651 184 2007 Fragments arranged by size: 3651 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4020 Fragments arranged by size: 4020 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3800 4159 4318 518 Size: 648 2634 359 159 2042 Fragments arranged by size: 2634 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3258 3935 5083 5328 1606 Size: 1652 677 1148 245 2120 Fragments arranged by size: 2120 1652 1148 677 245 NaeI GCC'GGC Cuts at: 2061 2061 Size: 5842 NcoI C'CATG_G Cuts at: 513 1065 2004 2154 513 Size: 552 939 150 4201 Fragments arranged by size: 4201 939 552 150 NdeI CA'TA_TG Cuts at: 387 387 Size: 5842 NgoMIV G'CCGG_C Cuts at: 2059 2059 Size: 5842 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5842 NotI GC'GGCC_GC Cuts at: 3289 3289 Size: 5842 NspI r_CATG'y Cuts at: 1132 1229 2971 3200 4745 1132 Size: 97 1742 229 1545 2229 Fragments arranged by size: 2229 1742 1545 229 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5842 PciI A'CATG_T Cuts at: 4741 4741 Size: 5842 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5842 PmeI GTTT'AAAC Cuts at: 3146 3146 Size: 5842 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3911 3943 5424 5456 3911 Size: 32 1481 32 4297 Fragments arranged by size: 4297 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5842 PsiI TTA'TAA Cuts at: 3412 3412 Size: 5842 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2708 2740 2708 Size: 32 5810 PstI C_TGCA'G Cuts at: 830 2328 2667 3194 830 Size: 1498 339 527 3478 Fragments arranged by size: 3478 1498 527 339 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5842 SalI G'TCGA_C Cuts at: 1936 3184 1936 Size: 1248 4594 SbfI CC_TGCA'GG Cuts at: 3194 3194 Size: 5842 ScaI AGT'ACT Cuts at: 4090 5802 4090 Size: 1712 4130 SfcI C'TryA_G Cuts at: 826 1045 2324 2663 3190 4325 5006 5197 826 Size: 219 1279 339 527 1135 681 191 1471 Fragments arranged by size: 1471 1279 1135 681 527 339 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5842 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5842 SmaI CCC'GGG Cuts at: 1872 2053 2366 3169 1872 Size: 181 313 803 4545 Fragments arranged by size: 4545 803 313 181 SmlI C'TyrA_G Cuts at: 820 1017 1943 3946 4847 5109 820 Size: 197 926 2003 901 262 1553 Fragments arranged by size: 2003 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3633 493 Size: 3140 2702 SpeI A'CTAG_T Cuts at: 152 2809 152 Size: 2657 3185 SphI G_CATG'C Cuts at: 1132 3200 1132 Size: 2068 3774 SrfI GCCC'GGGC Cuts at: 2053 2053 Size: 5842 SspI AAT'ATT Cuts at: 5 52 3068 3766 5 Size: 47 3016 698 2081 Fragments arranged by size: 3016 2081 698 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5842 StyI C'CwwG_G Cuts at: 513 1065 2004 2154 3281 513 Size: 552 939 150 1127 3074 Fragments arranged by size: 3074 1127 939 552 150 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2100 3869 3886 4039 4224 4669 325 Size: 1255 520 1769 17 153 185 445 1498 Fragments arranged by size: 1769 1498 1255 520 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2199 3133 4088 Size: 274 80 33 51 1443 222 934 955 Cuts at: 4088 5800 96 Size: 1712 138 Fragments arranged by size: 1712 1443 955 934 274 222 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5802 5834 5802 Size: 32 5810 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5842 XbaI T'CTAG_A Cuts at: 3178 3178 Size: 5842 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5842 XmaI C'CCGG_G Cuts at: 1870 2051 2364 3167 1870 Size: 181 313 803 4545 Fragments arranged by size: 4545 803 313 181 XmnI GAAnn'nnTTC Cuts at: 3000 3971 3000 Size: 971 4871 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3650 276 Size: 53 83 186 936 2116 2468 Fragments arranged by size: 2468 2116 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SwaI XcmI