(Linear) (Six-base) MAPSORT of: KIEE1859.seq Check: 8209 from: 1 to: 1137 KIEE1859.orf With 182 enzymes: SgfI * July 15, 2010 18:49 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 415 1137 Size: 415 722 ApoI r'AATT_y Cuts at: 0 283 559 634 1137 Size: 283 276 75 503 Fragments arranged by size: 503 283 276 75 AvaI C'yCGr_G Cuts at: 0 46 359 1137 Size: 46 313 778 Fragments arranged by size: 778 313 46 BanI G'GyrC_C Cuts at: 0 231 1137 Size: 231 906 BanII G_rGCy'C Cuts at: 0 47 1137 Size: 47 1090 BbsI GAAGACnn'nnnn_ Cuts at: 0 965 998 1137 Size: 965 33 139 Fragments arranged by size: 965 139 33 BsaBI GATnn'nnATC Cuts at: 0 224 1137 Size: 224 913 BsaHI Gr'CG_yC Cuts at: 0 142 1137 Size: 142 995 BsaWI w'CCGG_w Cuts at: 0 228 407 1137 Size: 228 179 730 Fragments arranged by size: 730 228 179 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 166 196 1137 Size: 166 30 941 Fragments arranged by size: 941 166 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 493 1137 Size: 493 644 BseYI C'CCAG_C Cuts at: 0 71 92 127 1137 Size: 71 21 35 1010 Fragments arranged by size: 1010 71 35 21 BsiEI CG_ry'CG Cuts at: 0 122 1137 Size: 122 1015 Bsp1286I G_dGCh'C Cuts at: 0 47 1137 Size: 47 1090 BspEI T'CCGG_A Cuts at: 0 407 1137 Size: 407 730 BsrFI r'CCGG_y Cuts at: 0 54 1137 Size: 54 1083 BsrGI T'GTAC_A Cuts at: 0 194 1128 1137 Size: 194 934 9 Fragments arranged by size: 934 194 9 BstXI CCAn_nnnn'nTGG Cuts at: 0 258 1137 Size: 258 879 BstYI r'GATC_y Cuts at: 0 225 438 540 1081 1137 Size: 225 213 102 541 56 Fragments arranged by size: 541 225 213 102 56 BtgI C'CryG_G Cuts at: 0 137 149 257 1137 Size: 137 12 108 880 Fragments arranged by size: 880 137 108 12 BtsI GCAGTG_nn' Cuts at: 0 464 1137 Size: 464 673 DraI TTT'AAA Cuts at: 0 639 1137 Size: 639 498 EaeI y'GGCC_r Cuts at: 0 5 119 1137 Size: 5 114 1018 Fragments arranged by size: 1018 114 5 EagI C'GGCC_G Cuts at: 0 119 1137 Size: 119 1018 EarI CTCTTCn'nnn_ Cuts at: 0 104 1137 Size: 104 1033 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 415 1137 Size: 415 722 EcoRV GAT'ATC Cuts at: 0 951 1137 Size: 951 186 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1015 1137 Size: 1015 122 FspI TGC'GCA Cuts at: 0 32 1137 Size: 32 1105 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 166 198 1137 Size: 166 32 939 Fragments arranged by size: 939 166 32 NaeI GCC'GGC Cuts at: 0 56 1137 Size: 56 1081 NcoI C'CATG_G Cuts at: 0 149 1137 Size: 149 988 NgoMIV G'CCGG_C Cuts at: 0 54 1137 Size: 54 1083 NspI r_CATG'y Cuts at: 0 966 1137 Size: 966 171 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 703 735 1137 Size: 703 32 402 Fragments arranged by size: 703 402 32 PstI C_TGCA'G Cuts at: 0 323 662 1137 Size: 323 339 475 Fragments arranged by size: 475 339 323 SfcI C'TryA_G Cuts at: 0 319 658 1137 Size: 319 339 479 Fragments arranged by size: 479 339 319 SmaI CCC'GGG Cuts at: 0 48 361 1137 Size: 48 313 776 Fragments arranged by size: 776 313 48 SpeI A'CTAG_T Cuts at: 0 804 1137 Size: 804 333 SrfI GCCC'GGGC Cuts at: 0 48 1137 Size: 48 1089 SspI AAT'ATT Cuts at: 0 1063 1137 Size: 1063 74 StyI C'CwwG_G Cuts at: 0 149 1137 Size: 149 988 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 95 1137 Size: 95 1042 TatI w'GTAC_w Cuts at: 0 194 1128 1137 Size: 194 934 9 Fragments arranged by size: 934 194 9 XmaI C'CCGG_G Cuts at: 0 46 359 1137 Size: 46 313 778 Fragments arranged by size: 778 313 46 XmnI GAAnn'nnTTC Cuts at: 0 995 1137 Size: 995 142 Enzymes that do cut: AcuI ApoI AvaI BanI BanII BbsI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI Bsp1286I BspEI BsrFI BsrGI BstXI BstYI BtgI BtsI DraI EaeI EagI EarI Eco57MI EcoRV FalI FspI Hin4I NaeI NcoI NgoMIV NspI PsrI PstI SfcI SmaI SpeI SrfI SspI StyI TaqII TatI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsgI BsiHKAI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrDI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EciI EcoICRI EcoNI EcoO109I EcoRI FseI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NdeI NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SphI StuI SwaI TstI Tth111I XbaI XcmI XhoI ZraI