Restriction map for
pFN21AE1888
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BamHI BbeI BbrPI
BcgI BciVI BfaI BfrI BglI
BlnI BsaBI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstUI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII EagI
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HincII HindII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MseI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccIII 1022 TCCGGA T^CCGGA
AcyI 1078 GACGCC G[GA]^CG[CT]C
AfaI 927 GTAC GT^AC
AhdI 181 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 150 GGATC GGATC(4/5)
ApaI 409 GGGCCC GGGCC^C
AspEI 181 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1066 GAGCAC G[AT]GC[AT]^C
BclI 980 TGATCA T^GATCA
BglII 155 AGATCT A^GATCT
BsaAI 1198 TACGTG [CT]AC^GT[GA]
BsaHI 1078 GACGCC G[GA]^CG[CT]C
BsaI 1237 GAGACC GGTCTC(1/5)
BsaWI 1022 TCCGGA [AT]^CCGG[AT]
BseAI 1022 TCCGGA T^CCGGA
BsgI 113 GTGCAG GTGCAG(16/14)
BsiHKAI 1066 GAGCAC G[AT]GC[AT]^C
BsmFI 473 GGGAC GGGAC(10/14)
BspEI 1022 TCCGGA T^CCGGA
BstAPI 762 GCAGAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 212 TTCGAA TT^CGAA
BstXI 413 CCATGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 155 AGATCT [GA]^GATC[CT]
DrdI 475 GACCTGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 181 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 413 CCATGG C^C[AT][AT]GG
FauI 469 GCGGG CCCGC(4/6)
FbaI 980 TGATCA T^GATCA
Hin1I 1078 GACGCC G[GA]^CG[CT]C
HindIII 553 AAGCTT A^AGCTT
MflI 155 AGATCT [GA]^GATC[CT]
MroI 1022 TCCGGA T^CCGGA
MslI 1035 CACCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 413 CCATGG C^CATGG
NspV 212 TTCGAA TT^CGAA
PflMI 741 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 927 GTAC GT^AC
SapI 969 GCTCTTC GCTCTTC(1/4)
SfuI 212 TTCGAA TT^CGAA
SmlI 1222 CTCAAG C^T[CT][GA]AG
StyI 413 CCATGG C^C[AT][AT]GG
Tsp509I 255 AATT ^AATT
Van91I 741 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 155 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AvaI 135 CCCGAG C^[CT]CG[GA]G
235 CCCGAG
BalI 644 TGGCCA TGG^CCA
1139 TGGCCA
BbsI 178 GAAGAC GAAGAC(2/6)
209 GTCTTC
BbvCI 1167 CCTCAGC CCTCAGC(-5/-2)
244 GCTGAGG
BlpI 562 GCTGAGC GC^T[ACGT]AGC
1100 GCTCAGC
Bpu1102I 562 GCTGAGC GC^T[ACGT]AGC
1100 GCTCAGC
BpuAI 178 GAAGAC GAAGAC(2/6)
209 GTCTTC
BseRI 520 GAGGAG GAGGAG(10/8)
781 GAGGAG
BsmAI 1237 GAGAC GTCTC(1/5)
122 GTCTC
BsoBI 135 CCCGAG C^[CT]CG[GA]G
235 CCCGAG
BstEII 324 GGTCACC G^GT[ACGT]ACC
1032 GGTCACC
BstF5I 684 CATCC GGATG(2/0)
1147 GGATG
BstPI 324 GGTCACC G^GT[ACGT]ACC
1032 GGTCACC
CelII 562 GCTGAGC GC^T[ACGT]AGC
1100 GCTCAGC
EaeI 644 TGGCCA [CT]^GGCC[GA]
1139 TGGCCA
EcoO65I 324 GGTCACC G^GT[ACGT]ACC
1032 GGTCACC
FokI 684 CATCC GGATG(9/13)
1147 GGATG
HgaI 1078 GACGC GACGC(5/10)
1105 GCGTC
HinfI 403 GAGTC G^A[ACGT]TC
844 GAGTC
MaeII 1136 ACGT A^CGT
1199 ACGT
MluNI 644 TGGCCA TGG^CCA
1139 TGGCCA
MscI 644 TGGCCA TGG^CCA
1139 TGGCCA
MspA1I 445 CAGCTG C[AC]G^C[GT]G
667 CAGCTG
PleI 403 GAGTC GAGTC(4/5)
844 GAGTC
PpuMI 83 AGGACCC [GA]G^G[AT]CC[CT]
473 GGGACCT
PvuII 445 CAGCTG CAG^CTG
667 CAGCTG
StuI 626 AGGCCT AGG^CCT
785 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AluI AlwNI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BsiYI BslI Bsp1286I BspHI
BspMI BsrI BssKI BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI EcoNI EcoO109I EcoRII
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PstI RcaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TseI Tsp45I
TspRI TthHB8I