(Linear) (Six-base) MAPSORT of: KIEE1888.seq Check: 6047 from: 1 to: 1248 KIEE1888.orf With 182 enzymes: SgfI * July 15, 2010 18:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 262 1248 Size: 262 986 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 700 1248 Size: 700 548 AhdI GACnn_n'nnGTC Cuts at: 0 186 1248 Size: 186 1062 AlwNI CAG_nnn'CTG Cuts at: 0 432 726 747 1248 Size: 432 294 21 501 Fragments arranged by size: 501 432 294 21 ApaI G_GGCC'C Cuts at: 0 413 1248 Size: 413 835 AvaI C'yCGr_G Cuts at: 0 135 235 1248 Size: 135 100 1013 Fragments arranged by size: 1013 135 100 BanI G'GyrC_C Cuts at: 0 62 260 498 836 1248 Size: 62 198 238 338 412 Fragments arranged by size: 412 338 238 198 62 BanII G_rGCy'C Cuts at: 0 98 105 413 1248 Size: 98 7 308 835 Fragments arranged by size: 835 308 98 7 BbsI GAAGACnn'nnnn_ Cuts at: 0 185 202 1248 Size: 185 17 1046 Fragments arranged by size: 1046 185 17 BbvCI CC'TCA_GC Cuts at: 0 245 1168 1248 Size: 245 923 80 Fragments arranged by size: 923 245 80 BclI T'GATC_A Cuts at: 0 980 1248 Size: 980 268 BglII A'GATC_T Cuts at: 0 155 1248 Size: 155 1093 BlpI GC'TnA_GC Cuts at: 0 563 1101 1248 Size: 563 538 147 Fragments arranged by size: 563 538 147 Bme1580I G_kGCm'C Cuts at: 0 67 265 413 501 1248 Size: 67 198 148 88 747 Fragments arranged by size: 747 198 148 88 67 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 345 804 1248 Size: 345 459 444 Fragments arranged by size: 459 444 345 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 169 421 442 499 589 778 1248 Size: 169 252 21 57 90 189 470 Fragments arranged by size: 470 252 189 169 90 57 21 Bpu10I CC'TnA_GC Cuts at: 0 12 245 688 1096 1168 1248 Size: 12 233 443 408 72 80 Fragments arranged by size: 443 408 233 80 72 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1207 1248 Size: 1207 41 BsaI GGTCTCn'nnnn_ Cuts at: 0 1231 1248 Size: 1231 17 BsaAI yAC'GTr Cuts at: 0 1200 1248 Size: 1200 48 BsaHI Gr'CG_yC Cuts at: 0 1079 1248 Size: 1079 169 BsaWI w'CCGG_w Cuts at: 0 1022 1248 Size: 1022 226 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 535 796 1248 Size: 535 261 452 Fragments arranged by size: 535 452 261 BseYI C'CCAG_C Cuts at: 0 387 879 1248 Size: 387 492 369 Fragments arranged by size: 492 387 369 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 134 1248 Size: 134 1114 BsiHKAI G_wGCw'C Cuts at: 0 1070 1248 Size: 1070 178 Bsp1286I G_dGCh'C Cuts at: 0 67 98 105 265 413 501 1070 1248 Size: 67 31 7 160 148 88 569 178 Fragments arranged by size: 569 178 160 148 88 67 31 7 BspEI T'CCGG_A Cuts at: 0 1022 1248 Size: 1022 226 BspHI T'CATG_A Cuts at: 0 365 542 983 1248 Size: 365 177 441 265 Fragments arranged by size: 441 365 265 177 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 262 359 449 1248 Size: 262 97 90 799 Fragments arranged by size: 799 262 97 90 BstAPI GCAn_nnn'nTGC Cuts at: 0 768 1248 Size: 768 480 BstBI TT'CG_AA Cuts at: 0 213 1248 Size: 213 1035 BstEII G'GTnAC_C Cuts at: 0 324 1032 1248 Size: 324 708 216 Fragments arranged by size: 708 324 216 BstXI CCAn_nnnn'nTGG Cuts at: 0 420 1248 Size: 420 828 BstYI r'GATC_y Cuts at: 0 155 1248 Size: 155 1093 BtgI C'CryG_G Cuts at: 0 413 458 1012 1248 Size: 413 45 554 236 Fragments arranged by size: 554 413 236 45 BtsI GCAGTG_nn' Cuts at: 0 890 1248 Size: 890 358 DrdI GACnn_nn'nnGTC Cuts at: 0 481 1248 Size: 481 767 EaeI y'GGCC_r Cuts at: 0 644 1139 1248 Size: 644 495 109 Fragments arranged by size: 644 495 109 EarI CTCTTCn'nnn_ Cuts at: 0 877 976 1162 1248 Size: 877 99 186 86 Fragments arranged by size: 877 186 99 86 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 169 421 442 499 589 700 778 1248 Size: 169 252 21 57 90 111 78 470 Fragments arranged by size: 470 252 169 111 90 78 57 21 EcoNI CCTnn'n_nnAGG Cuts at: 0 613 778 855 1113 1248 Size: 613 165 77 258 135 Fragments arranged by size: 613 258 165 135 77 EcoO109I rG'GnC_Cy Cuts at: 0 84 474 744 1118 1248 Size: 84 390 270 374 130 Fragments arranged by size: 390 374 270 130 84 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1053 1248 Size: 1053 195 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 172 204 945 977 1084 1116 1139 1171 Size: 172 32 741 32 107 32 23 32 Cuts at: 1171 1248 Size: 77 Fragments arranged by size: 741 172 107 77 32 32 32 32 23 HindIII A'AGCT_T Cuts at: 0 553 1248 Size: 553 695 MscI TGG'CCA Cuts at: 0 646 1141 1248 Size: 646 495 107 Fragments arranged by size: 646 495 107 MslI CAynn'nnrTG Cuts at: 0 1039 1248 Size: 1039 209 MspA1I CmG'CkG Cuts at: 0 447 669 1248 Size: 447 222 579 Fragments arranged by size: 579 447 222 NcoI C'CATG_G Cuts at: 0 413 1248 Size: 413 835 PasI CC'CwG_GG Cuts at: 0 98 747 1248 Size: 98 649 501 Fragments arranged by size: 649 501 98 PflMI CCAn_nnn'nTGG Cuts at: 0 747 1248 Size: 747 501 PpuMI rG'GwC_Cy Cuts at: 0 84 474 1248 Size: 84 390 774 Fragments arranged by size: 774 390 84 PspOMI G'GGCC_C Cuts at: 0 409 1248 Size: 409 839 PstI C_TGCA'G Cuts at: 0 446 452 536 1181 1248 Size: 446 6 84 645 67 Fragments arranged by size: 645 446 84 67 6 PvuII CAG'CTG Cuts at: 0 447 669 1248 Size: 447 222 579 Fragments arranged by size: 579 447 222 SapI GCTCTTCn'nnn_ Cuts at: 0 976 1248 Size: 976 272 SfcI C'TryA_G Cuts at: 0 442 448 532 1177 1248 Size: 442 6 84 645 71 Fragments arranged by size: 645 442 84 71 6 SmlI C'TyrA_G Cuts at: 0 1222 1248 Size: 1222 26 StuI AGG'CCT Cuts at: 0 628 787 1248 Size: 628 159 461 Fragments arranged by size: 628 461 159 StyI C'CwwG_G Cuts at: 0 413 1248 Size: 413 835 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 50 248 1200 1248 Size: 50 198 952 48 Fragments arranged by size: 952 198 50 48 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 567 599 1145 1177 1248 Size: 567 32 546 32 71 Fragments arranged by size: 567 546 71 32 32 Enzymes that do cut: AarI AcuI AhdI AlwNI ApaI AvaI BanI BanII BbsI BbvCI BclI BglII BlpI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI BstAPI BstBI BstEII BstXI BstYI BtgI BtsI DrdI EaeI EarI Eco57MI EcoNI EcoO109I FalI Hin4I HindIII MscI MslI MspA1I NcoI PasI PflMI PpuMI PspOMI PstI PvuII SapI SfcI SmlI StuI StyI TaqII TstI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BfrBI BglI BmgBI BmrI BmtI BsaBI BsaXI BsiEI BsiWI BsmI BsmBI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HaeII HincII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI