################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## Acc65I AccI AccII AclI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsgI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI FbaI FseI HaeII HgaI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MscI MseI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuII RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I TspRI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 543 GACGTC GACGT^C AccIII 231 TCCGGA T^CCGGA AcsI 456 GAATTC [GA]^AATT[CT] AcyI 543 GACGTC G[GA]^CG[CT]C AlwI 561 GGATC GGATC(4/5) ApoI 456 GAATTC [GA]^AATT[CT] AvaI 525 CTCGGG C^[CT]CG[GA]G AvaII 144 GGTCC G^G[AT]CC AviII 83 TGCGCA TGC^GCA BanI 379 GGCACC G^G[CT][GA]CC BcnI 36 CCGGG CC^[GC]GG BsaHI 543 GACGTC G[GA]^CG[CT]C BsaWI 231 TCCGGA [AT]^CCGG[AT] BseAI 231 TCCGGA T^CCGGA BseRI 30 GAGGAG GAGGAG(10/8) BsiEI 71 CGATCG CG[GA][CT]^CG BsmFI 145 GTCCC GGGAC(10/14) BsoBI 525 CTCGGG C^[CT]CG[GA]G BspEI 231 TCCGGA T^CCGGA BspMI 8 GCAGGT ACCTGC(4/8) BsrBI 344 CCGCTC CCGCTC(-3/-3) BsrI 597 ACTGG ACTGG(1/-1) BstXI 405 CCAGGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 561 GGATCT [GA]^GATC[CT] CfoI 84 GCGC GCG^C DraII 143 GGGTCCC [GA]G^G[ACGT]CC[CT] EaeI 533 TGGCCG [CT]^GGCC[GA] EarI 622 GAAGAG CTCTTC(1/4) EcoO109I 143 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoRI 456 GAATTC G^AATTC EcoRV 49 GATATC GAT^ATC EcoT22I 265 ATGCAT ATGCA^T FauI 343 CCCGC CCCGC(4/6) FspI 83 TGCGCA TGC^GCA HhaI 84 GCGC GCG^C Hin1I 543 GACGTC G[GA]^CG[CT]C HinP1I 84 GCGC G^CGC Ksp632I 622 GAAGAG CTCTTC(1/4) MaeII 544 ACGT A^CGT MflI 561 GGATCT [GA]^GATC[CT] MroI 231 TCCGGA T^CCGGA NciI 36 CCGGG CC^[GC]GG NsiI 265 ATGCAT ATGCA^T NspI 263 ACATGC [GA]CATG^[CT] PleI 523 GACTC GAGTC(4/5) PpuMI 143 GGGTCCC [GA]G^G[AT]CC[CT] PshAI 523 GACTCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 71 CGATCG CGAT^CG SacI 288 GAGCTC GAGCT^C StuI 602 AGGCCT AGG^CCT TfiI 131 GAATC G^A[AT]TC Tsp509I 457 AATT ^AATT XhoII 561 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AlwNI 428 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 559 CAGGATCTG AspHI 288 GAGCTC G[AT]GC[AT]^C 421 GAGCAC BbvI 329 GCAGC GCAGC(8/12) 510 GCAGC BsiHKAI 288 GAGCTC G[AT]GC[AT]^C 421 GAGCAC BsmBI 25 GAGACG CGTCTC(1/5) 541 GAGACG BstEII 105 GGTGACC G^GT[ACGT]ACC 222 GGTGACC BstPI 105 GGTGACC G^GT[ACGT]ACC 222 GGTGACC EcoO65I 105 GGTGACC G^GT[ACGT]ACC 222 GGTGACC EcoT14I 161 CCAAGG C^C[AT][AT]GG 389 CCAAGG HapII 36 CCGG C^CGG 232 CCGG HinfI 131 GAATC G^A[ACGT]TC 523 GACTC HpaII 36 CCGG C^CGG 232 CCGG MspI 36 CCGG C^CGG 232 CCGG SfaNI 301 GATGC GCATC(5/9) 439 GATGC StyI 161 CCAAGG C^C[AT][AT]GG 389 CCAAGG TseI 329 GCAGC G^C[AT]GC 510 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AciI AluI BanII BmyI BpmI BsaJI BsiYI BslI BsmAI Bsp1286I BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HphI ItaI MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TaqI Tsp45I TthHB8I