Restriction map for
pFN21AE1721
(Linear) (Six-base) MAPSORT of: KIEE1721.seq Check: 6268 from: 1 to: 630
KIEE1721.orf
With 182 enzymes: SgfI *
July 15, 2010 18:46 ..
AatII G_ACGT'C
Cuts at: 0 547 630
Size: 547 83
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 630
Size: 455 175
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 229 261 630
Size: 229 32 369
Fragments arranged by size:
369 229 32
AlwNI CAG_nnn'CTG
Cuts at: 0 433 564 630
Size: 433 131 66
Fragments arranged by size:
433 131 66
ApoI r'AATT_y
Cuts at: 0 456 630
Size: 456 174
AvaI C'yCGr_G
Cuts at: 0 525 630
Size: 525 105
BanI G'GyrC_C
Cuts at: 0 379 630
Size: 379 251
BanII G_rGCy'C
Cuts at: 0 161 292 356 630
Size: 161 131 64 274
Fragments arranged by size:
274 161 131 64
BfrBI ATG'CAT
Cuts at: 0 267 630
Size: 267 363
Bme1580I G_kGCm'C
Cuts at: 0 371 630
Size: 371 259
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 375 630
Size: 375 255
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 321 332 539 619 630
Size: 321 11 207 80 11
Fragments arranged by size:
321 207 80 11 11
BsaHI Gr'CG_yC
Cuts at: 0 544 630
Size: 544 86
BsaWI w'CCGG_w
Cuts at: 0 231 630
Size: 231 399
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 229 259 274 304 630
Size: 229 30 15 30 326
Fragments arranged by size:
326 229 30 30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 45 630
Size: 45 585
BseYI C'CCAG_C
Cuts at: 0 370 630
Size: 370 260
BsiEI CG_ry'CG
Cuts at: 0 74 630
Size: 74 556
BsiHKAI G_wGCw'C
Cuts at: 0 292 425 630
Size: 292 133 205
Fragments arranged by size:
292 205 133
BsmBI CGTCTCn'nnnn_
Cuts at: 0 19 535 630
Size: 19 516 95
Fragments arranged by size:
516 95 19
Bsp1286I G_dGCh'C
Cuts at: 0 161 292 356 371 425 630
Size: 161 131 64 15 54 205
Fragments arranged by size:
205 161 131 64 54 15
BspEI T'CCGG_A
Cuts at: 0 231 630
Size: 231 399
BsrBI CCG'CTC
Cuts at: 0 346 630
Size: 346 284
BstEII G'GTnAC_C
Cuts at: 0 105 222 630
Size: 105 117 408
Fragments arranged by size:
408 117 105
BstXI CCAn_nnnn'nTGG
Cuts at: 0 412 630
Size: 412 218
BstYI r'GATC_y
Cuts at: 0 561 630
Size: 561 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 279 630
Size: 279 351
EaeI y'GGCC_r
Cuts at: 0 533 630
Size: 533 97
EarI CTCTTCn'nnn_
Cuts at: 0 617 630
Size: 617 13
EcoICRI GAG'CTC
Cuts at: 0 290 630
Size: 290 340
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 321 332 455 539 619 630
Size: 321 11 123 84 80 11
Fragments arranged by size:
321 123 84 80 11 11
EcoO109I rG'GnC_Cy
Cuts at: 0 144 630
Size: 144 486
EcoRI G'AATT_C
Cuts at: 0 456 630
Size: 456 174
EcoRV GAT'ATC
Cuts at: 0 51 630
Size: 51 579
FspI TGC'GCA
Cuts at: 0 85 630
Size: 85 545
NsiI A_TGCA'T
Cuts at: 0 269 630
Size: 269 361
NspI r_CATG'y
Cuts at: 0 267 630
Size: 267 363
PasI CC'CwG_GG
Cuts at: 0 250 630
Size: 250 380
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 229 261 630
Size: 229 32 369
Fragments arranged by size:
369 229 32
PpuMI rG'GwC_Cy
Cuts at: 0 144 630
Size: 144 486
PshAI GACnn'nnGTC
Cuts at: 0 527 630
Size: 527 103
PvuI CG_AT'CG
Cuts at: 0 74 630
Size: 74 556
SacI G_AGCT'C
Cuts at: 0 292 630
Size: 292 338
SanDI GG'GwC_CC
Cuts at: 0 144 630
Size: 144 486
StuI AGG'CCT
Cuts at: 0 604 630
Size: 604 26
StyI C'CwwG_G
Cuts at: 0 161 389 630
Size: 161 228 241
Fragments arranged by size:
241 228 161
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 93 440 630
Size: 93 347 190
Fragments arranged by size:
347 190 93
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 193 225 630
Size: 193 32 405
Fragments arranged by size:
405 193 32
ZraI GAC'GTC
Cuts at: 0 545 630
Size: 545 85
Enzymes that do cut:
AatII AcuI AloI AlwNI ApoI AvaI BanI BanII
BfrBI Bme1580I BplI BpmI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BsrBI BstEII
BstXI BstYI BtgZI EaeI EarI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FspI NsiI NspI PasI PpiI PpuMI
PshAI PvuI SacI SanDI StuI StyI TaqII TstI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI BmgBI BmrI BmtI Bpu10I
BpuEI BsaI BsaAI BsaBI BsgI BsiWI BsmI BspHI
BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoNI FalI FseI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI PciI PflMI PfoI
PmeI PmlI PsiI PspOMI PspXI PsrI PstI PvuII
RsrII SacII SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TatI Tth111I XbaI XcmI
XhoI XmaI XmnI