Restriction map for
pFN21AE1865
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI AlwNI
AocI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BanI BbeI BbvCI
BcgI BciVI BclI BfaI BfrI
BlnI BlpI Bpu1102I BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BsrDI
BsrFI BssHII BssSI Bst1107I BstBI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRV EcoT22I FbaI
FseI FspI Hin1I HindIII HinfI
HpaI KasI KpnI KspI MaeI
MfeI MluI MroI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PleI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SspI SwaI TfiI Tth111I
Van91I XbaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 1128 GTCTAC GT^[AC][GT]AC
AfaI 78 GTAC GT^AC
Alw44I 601 GTGCAC G^TGCAC
ApaI 389 GGGCCC GGGCC^C
ApaLI 601 GTGCAC G^TGCAC
BalI 50 TGGCCA TGG^CCA
BpmI 760 CTGGAG CTGGAG(16/14)
BsaBI 48 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 77 TGTACA T^GTACA
BspHI 662 TCATGA T^CATGA
BspLU11I 74 ACATGT A^CATGT
BsrBI 504 CCGCTC CCGCTC(-3/-3)
BsrGI 77 TGTACA T^GTACA
BsrI 780 CCAGT ACTGG(1/-1)
BstAPI 594 GCATATCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 946 GGTGACC G^GT[ACGT]ACC
BstPI 946 GGTGACC G^GT[ACGT]ACC
DdeI 1145 CTGAG C^T[ACGT]AG
DraIII 517 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 50 TGGCCA [CT]^GGCC[GA]
EarI 162 CTCTTC CTCTTC(1/4)
EcoO65I 946 GGTGACC G^GT[ACGT]ACC
EcoT14I 791 CCAAGG C^C[AT][AT]GG
HgaI 320 GCGTC GACGC(5/10)
HincII 470 GTCAAC GT[CT]^[GA]AC
HindII 470 GTCAAC GT[CT]^[GA]AC
Ksp632I 162 CTCTTC CTCTTC(1/4)
MamI 48 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 50 TGGCCA TGG^CCA
MscI 50 TGGCCA TGG^CCA
MseI 983 TTAA T^TAA
PaeR7I 839 CTCGAG C^TCGAG
PvuII 757 CAGCTG CAG^CTG
RcaI 662 TCATGA T^CATGA
RsaI 78 GTAC GT^AC
SapI 161 GCTCTTC GCTCTTC(1/4)
SbfI 798 CCTGCAGG CCTGCA^GG
SphI 125 GCATGC GCATG^C
Sse8387I 798 CCTGCAGG CCTGCA^GG
SspBI 77 TGTACA T^GTACA
StuI 497 AGGCCT AGG^CCT
StyI 791 CCAAGG C^C[AT][AT]GG
Tru9I 983 TTAA T^TAA
XcmI 1058 CCATGAATTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 839 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AflIII 74 ACATGT A^C[GA][CT]GT
230 ACGTGT
AlwI 285 GGATC GGATC(4/5)
1119 GGATC
BbsI 144 GTCTTC GAAGAC(2/6)
301 GTCTTC
BpuAI 144 GTCTTC GAAGAC(2/6)
301 GTCTTC
BsmAI 354 GAGAC GTCTC(1/5)
988 GTCTC
DrdI 313 GACATGAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
774 GACCATCCAGTC
EcoRI 874 GAATTC G^AATTC
889 GAATTC
HaeII 152 GGCGCT [GA]GCGC^[CT]
1001 GGCGCT
HphI 946 GGTGA GGTGA(8/7)
926 TCACC
MaeIII 413 GTGAC ^GT[ACGT]AC
947 GTGAC
MspA1I 565 CCGCTG C[AC]G^C[GT]G
757 CAGCTG
NspI 74 ACATGT [GA]CATG^[CT]
125 GCATGC
PpuMI 614 GGGACCC [GA]G^G[AT]CC[CT]
830 AGGACCT
SexAI 342 ACCTGGT A^CC[AT]GGT
605 ACCTGGT
SmaI 443 CCCGGG CCC^GGG
620 CCCGGG
TaqI 840 TCGA T^CGA
1032 TCGA
Tsp45I 413 GTGAC ^GT[GC]AC
947 GTGAC
TspRI 373 CAGTG CA[GC]TG[ACGT][ACGT]^
451 CACTG
TthHB8I 840 TCGA T^CGA
1032 TCGA
XmaCI 443 CCCGGG C^CCGGG
620 CCCGGG
XmaI 443 CCCGGG C^CCGGG
620 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AluI ApoI AspHI
AvaI AvaII BanII BbrPI BbvI
BcnI BglI BglII BmyI BsaAI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DpnII DraII EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI MaeII
MboI MboII MflI MnlI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PmaCI PmlI PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TseI Tsp509I XhoII