(Circular) (Six-base) MAPSORT of: pFN21AE1865.seq Check: 1167 from: 1 to: 5863 pFN21AE1865 5863 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2419 2419 Size: 5863 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3673 278 Size: 53 83 186 936 2137 2468 Fragments arranged by size: 2468 2137 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3134 3206 1937 Size: 1197 72 4594 Fragments arranged by size: 4594 1197 72 Acc65I G'GTAC_C Cuts at: 3184 3184 Size: 5863 AclI AA'CG_TT Cuts at: 1563 3990 4363 1563 Size: 2427 373 3063 Fragments arranged by size: 3063 2427 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2894 3026 3926 5310 5578 1632 Size: 1262 132 900 1384 268 1917 Fragments arranged by size: 1917 1384 1262 900 268 132 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5666 AflIII A'CryG_T Cuts at: 1203 2079 2235 4762 5739 1203 Size: 876 156 2527 977 1327 Fragments arranged by size: 2527 1327 977 876 156 AgeI A'CCGG_T Cuts at: 4694 4694 Size: 5863 AhdI GACnn_n'nnGTC Cuts at: 4592 4592 Size: 5863 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5863 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2153 1184 Size: 969 4894 AlwNI CAG_nnn'CTG Cuts at: 3606 5178 3606 Size: 1572 4291 ApaI G_GGCC'C Cuts at: 2398 2398 Size: 5863 ApaLI G'TGCA_C Cuts at: 2606 3920 5076 2606 Size: 1314 1156 3393 Fragments arranged by size: 3393 1314 1156 ApoI r'AATT_y Cuts at: 1493 2879 2894 3067 3172 3398 3550 5659 1493 Size: 1386 15 173 105 226 152 2109 1697 Fragments arranged by size: 2109 1697 1386 226 173 152 105 15 AseI AT'TA_AT Cuts at: 160 1032 4417 160 Size: 872 3385 1606 Fragments arranged by size: 3385 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5863 AvaI C'yCGr_G Cuts at: 1870 1943 2123 2448 2625 2643 2844 2934 3188 Size: 73 180 325 177 18 201 90 254 Cuts at: 3188 1870 Size: 4545 Fragments arranged by size: 4545 325 254 201 180 177 90 73 18 BamHI G'GATC_C Cuts at: 3193 3560 3193 Size: 367 5496 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3184 4639 618 Size: 325 217 627 1397 1455 1842 Fragments arranged by size: 1842 1455 1397 627 325 217 BanII G_rGCy'C Cuts at: 1391 2226 2398 2455 2851 3182 1391 Size: 835 172 57 396 331 4072 Fragments arranged by size: 4072 835 396 331 172 57 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2142 2299 928 Size: 971 243 157 4492 Fragments arranged by size: 4492 971 243 157 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4054 4088 1903 Size: 34 50 34 2033 34 3678 Fragments arranged by size: 3678 2033 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3755 4965 3755 Size: 1210 4653 BclI T'GATC_A Cuts at: 1548 1548 Size: 5863 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2063 2230 2855 Size: 107 122 71 631 605 391 167 625 Cuts at: 2855 4474 136 Size: 1619 1525 Fragments arranged by size: 1619 1525 631 625 605 391 167 122 107 71 BglII A'GATC_T Cuts at: 2662 2769 3092 5858 2662 Size: 107 323 2766 2667 Fragments arranged by size: 2766 2667 323 107 BlpI GC'TnA_GC Cuts at: 3280 3280 Size: 5863 Bme1580I G_kGCm'C Cuts at: 1790 2398 2610 3924 5080 1790 Size: 608 212 1314 1156 2573 Fragments arranged by size: 2573 1314 1156 608 212 BmgBI CAC'GTC Cuts at: 1318 3088 1318 Size: 1770 4093 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3594 4552 442 Size: 416 53 83 399 24 2177 958 1753 Fragments arranged by size: 2177 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5863 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2786 4523 1239 Size: 1547 1737 2579 Fragments arranged by size: 2579 1737 1547 Bpu10I CC'TnA_GC Cuts at: 3150 3150 Size: 5863 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2516 2696 3988 4853 5151 2516 Size: 180 1292 865 298 3228 Fragments arranged by size: 3228 1292 865 298 180 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4526 882 Size: 617 3027 2219 Fragments arranged by size: 3027 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2137 2236 2524 3654 493 Size: 711 933 99 288 1130 2702 Fragments arranged by size: 2702 1130 933 711 288 99 BsaBI GATnn'nnATC Cuts at: 2057 5857 2057 Size: 3800 2063 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3670 4052 4687 275 Size: 53 83 186 936 2137 382 635 1451 Fragments arranged by size: 2137 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4296 4694 4968 5115 5526 4296 Size: 398 274 147 411 4633 Fragments arranged by size: 4633 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2084 2114 1178 Size: 30 165 30 681 30 4927 Fragments arranged by size: 4927 681 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5863 BseYI C'CCAG_C Cuts at: 3073 5066 5562 3073 Size: 1993 496 3374 Fragments arranged by size: 3374 1993 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2258 2391 2491 1163 Size: 573 522 133 100 4535 Fragments arranged by size: 4535 573 522 133 100 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3313 4074 5102 1540 Size: 275 186 1312 761 1028 2301 Fragments arranged by size: 2301 1312 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2261 2532 2610 3924 4009 5080 2261 Size: 271 78 1314 85 1071 3044 Fragments arranged by size: 3044 1314 1071 271 85 78 BsmI GAATG_Cn' Cuts at: 3374 3467 3374 Size: 93 5770 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2226 2261 2398 2455 2532 2610 2851 Size: 399 436 35 137 57 77 78 241 Cuts at: 2851 3182 3924 4009 5080 1391 Size: 331 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 436 399 331 241 137 85 78 77 57 35 BspEI T'CCGG_A Cuts at: 5526 5526 Size: 5863 BspHI T'CATG_A Cuts at: 2667 3752 5482 2667 Size: 1085 1730 3048 Fragments arranged by size: 3048 1730 1085 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2419 2562 2604 2734 2826 3218 844 Size: 1575 143 42 130 92 392 3489 Fragments arranged by size: 3489 1575 392 143 130 92 42 BsrBI CCG'CTC Cuts at: 1394 1823 2511 3750 1394 Size: 429 688 1239 3507 Fragments arranged by size: 3507 1239 688 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4358 4532 65 Size: 1183 3110 174 1396 Fragments arranged by size: 3110 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4507 4694 1700 Size: 110 2697 187 2869 Fragments arranged by size: 2869 2697 187 110 BsrGI T'GTAC_A Cuts at: 96 2082 96 Size: 1986 3877 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5863 BssSI C'ACGA_G Cuts at: 3923 4935 3923 Size: 1012 4851 BstAPI GCAn_nnn'nTGC Cuts at: 794 2605 794 Size: 1811 4052 BstBI TT'CG_AA Cuts at: 4667 4667 Size: 5863 BstEII G'GTnAC_C Cuts at: 1184 2951 1184 Size: 1767 4096 BstYI r'GATC_y Cuts at: 1972 2290 2662 2769 3092 3124 3193 3560 3945 Size: 318 372 107 323 32 69 367 385 Cuts at: 3945 3962 5414 5500 5512 5858 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 372 367 346 323 318 107 86 69 32 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5311 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3072 487 Size: 683 56 1846 3278 Fragments arranged by size: 3278 1846 683 56 BtsI GCAGTG_nn' Cuts at: 2455 3379 4173 4193 2455 Size: 924 794 20 4125 Fragments arranged by size: 4125 924 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5863 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5828 DraI TTT'AAA Cuts at: 3167 3167 Size: 5863 DraIII CAC_nnn'GTG Cuts at: 2527 2527 Size: 5863 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2324 2785 4870 809 Size: 507 1008 461 2085 1802 Fragments arranged by size: 2085 1802 1008 507 461 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 3310 4199 Size: 54 1425 50 275 18 225 1255 889 Cuts at: 4199 5828 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1255 889 275 225 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3310 1537 Size: 275 1498 4090 Fragments arranged by size: 4090 1498 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2173 3793 1353 Size: 820 1620 3423 Fragments arranged by size: 3423 1620 820 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4450 4824 4970 387 Size: 1064 138 2861 374 146 1280 Fragments arranged by size: 2861 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2786 2894 3026 3926 4523 5310 5578 Size: 393 1154 108 132 900 597 787 268 Cuts at: 5578 1239 Size: 1524 Fragments arranged by size: 1524 1154 900 787 597 393 268 132 108 EcoO109I rG'GnC_Cy Cuts at: 2395 2620 2800 2836 2395 Size: 225 180 36 5422 Fragments arranged by size: 5422 225 180 36 EcoRI G'AATT_C Cuts at: 2879 2894 3172 3550 2879 Size: 15 278 378 5192 Fragments arranged by size: 5192 378 278 15 FspI TGC'GCA Cuts at: 4369 4369 Size: 5863 HaeII r_GCGC'y Cuts at: 1678 2161 3010 5010 1678 Size: 483 849 2000 2531 Fragments arranged by size: 2531 2000 849 483 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2662 2694 3116 3148 4504 Size: 32 135 32 1090 32 422 32 1356 Cuts at: 4504 4536 4578 4610 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1356 1090 422 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2477 3207 3453 669 Size: 988 281 539 730 246 3079 Fragments arranged by size: 3079 988 730 539 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5863 HpaI GTT'AAC Cuts at: 3453 3453 Size: 5863 KpnI G_GTAC'C Cuts at: 3188 3188 Size: 5863 MfeI C'AATT_G Cuts at: 3462 3462 Size: 5863 MluI A'CGCG_T Cuts at: 5739 5739 Size: 5863 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4977 5161 1305 Size: 3672 184 2007 Fragments arranged by size: 3672 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2057 10 Size: 54 1425 343 225 3816 Fragments arranged by size: 3816 1425 343 225 54 MslI CAynn'nnrTG Cuts at: 518 1166 2135 2202 2282 2687 2970 3821 4180 Size: 648 969 67 80 405 283 851 359 Cuts at: 4180 4339 518 Size: 159 2042 Fragments arranged by size: 2042 969 851 648 405 359 283 159 80 67 MspA1I CmG'CkG Cuts at: 1606 2572 2764 3279 3956 5104 5349 1606 Size: 966 192 515 677 1148 245 2120 Fragments arranged by size: 2120 1148 966 677 515 245 192 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5311 NdeI CA'TA_TG Cuts at: 387 387 Size: 5863 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5863 NotI GC'GGCC_GC Cuts at: 3310 3310 Size: 5863 NspI r_CATG'y Cuts at: 1132 1229 2083 2134 3221 4766 1132 Size: 97 854 51 1087 1545 2229 Fragments arranged by size: 2229 1545 1087 854 97 51 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5863 PciI A'CATG_T Cuts at: 2079 4762 2079 Size: 2683 3180 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5863 PfoI T'CCnGG_A Cuts at: 2616 2616 Size: 5863 PmeI GTTT'AAAC Cuts at: 3167 3167 Size: 5863 PmlI CAC'GTG Cuts at: 2137 2236 2524 2137 Size: 99 288 5476 Fragments arranged by size: 5476 288 99 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3932 3964 5445 5477 3932 Size: 32 1481 32 4318 Fragments arranged by size: 4318 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2620 2836 2620 Size: 216 5647 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5863 PsiI TTA'TAA Cuts at: 3433 3433 Size: 5863 PspOMI G'GGCC_C Cuts at: 2394 2394 Size: 5863 PspXI vC'TCGA_Gb Cuts at: 2844 2844 Size: 5863 PstI C_TGCA'G Cuts at: 830 2462 2559 2808 3215 830 Size: 1632 97 249 407 3478 Fragments arranged by size: 3478 1632 407 249 97 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5863 PvuII CAG'CTG Cuts at: 2764 2764 Size: 5863 SalI G'TCGA_C Cuts at: 1936 3205 1936 Size: 1269 4594 SanDI GG'GwC_CC Cuts at: 2620 2620 Size: 5863 SapI GCTCTTCn'nnn_ Cuts at: 2173 2173 Size: 5863 SbfI CC_TGCA'GG Cuts at: 2808 3215 2808 Size: 407 5456 ScaI AGT'ACT Cuts at: 4111 5823 4111 Size: 1712 4151 SexAI A'CCwGG_T Cuts at: 2347 2610 2347 Size: 263 5600 SfcI C'TryA_G Cuts at: 826 1045 2458 2515 2555 2804 3211 4346 5027 Size: 219 1413 57 40 249 407 1135 681 Cuts at: 5027 5218 826 Size: 191 1471 Fragments arranged by size: 1471 1413 1135 681 407 249 219 191 57 40 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5863 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5863 SmaI CCC'GGG Cuts at: 1872 2450 2627 3190 1872 Size: 578 177 563 4545 Fragments arranged by size: 4545 578 563 177 SmlI C'TyrA_G Cuts at: 820 1017 1943 2531 2711 2844 3967 4868 5130 Size: 197 926 588 180 133 1123 901 262 Cuts at: 5130 820 Size: 1553 Fragments arranged by size: 1553 1123 926 901 588 262 197 180 133 SnaBI TAC'GTA Cuts at: 493 3654 493 Size: 3161 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5863 SphI G_CATG'C Cuts at: 1132 2134 3221 1132 Size: 1002 1087 3774 Fragments arranged by size: 3774 1087 1002 SspI AAT'ATT Cuts at: 5 52 3787 5 Size: 47 3735 2081 Fragments arranged by size: 3735 2081 47 StuI AGG'CCT Cuts at: 1517 2504 1517 Size: 987 4876 StyI C'CwwG_G Cuts at: 513 1065 2796 3302 513 Size: 552 1731 506 3074 Fragments arranged by size: 3074 1731 552 506 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2214 2908 2939 3890 3907 4060 4245 Size: 1255 634 694 31 951 17 153 185 Cuts at: 4245 4690 325 Size: 445 1498 Fragments arranged by size: 1498 1255 951 694 634 445 185 153 31 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2082 4109 5821 Size: 274 80 33 51 1443 105 2027 1712 Cuts at: 5821 96 Size: 138 Fragments arranged by size: 2027 1712 1443 274 138 105 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5823 5855 5823 Size: 32 5831 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5863 XbaI T'CTAG_A Cuts at: 3199 3199 Size: 5863 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3070 3070 Size: 5863 XhoI C'TCGA_G Cuts at: 1943 2844 1943 Size: 901 4962 XmaI C'CCGG_G Cuts at: 1870 2448 2625 3188 1870 Size: 578 177 563 4545 Fragments arranged by size: 4545 578 563 177 XmnI GAAnn'nnTTC Cuts at: 3992 3992 Size: 5863 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3671 276 Size: 53 83 186 936 2137 2468 Fragments arranged by size: 2468 2137 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PsrI RsrII SacI SacII SfiI SfoI SrfI SwaI