Restriction map for
pFN21AE1865
(Linear) (Six-base) MAPSORT of: KIEE1865.seq Check: 1585 from: 1 to: 1158
KIEE1865.orf
With 182 enzymes: SgfI *
July 15, 2010 18:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 414 1158
Size: 414 744
AccI GT'mk_AC
Cuts at: 0 1129 1158
Size: 1129 29
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 889 1021 1158
Size: 889 132 137
Fragments arranged by size:
889 137 132
AflIII A'CryG_T
Cuts at: 0 74 230 1158
Size: 74 156 928
Fragments arranged by size:
928 156 74
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 148 1158
Size: 148 1010
ApaI G_GGCC'C
Cuts at: 0 393 1158
Size: 393 765
ApaLI G'TGCA_C
Cuts at: 0 601 1158
Size: 601 557
ApoI r'AATT_y
Cuts at: 0 874 889 1062 1158
Size: 874 15 173 96
Fragments arranged by size:
874 173 96 15
AvaI C'yCGr_G
Cuts at: 0 118 443 620 638 839 929 1158
Size: 118 325 177 18 201 90 229
Fragments arranged by size:
325 229 201 177 118 90 18
BanII G_rGCy'C
Cuts at: 0 221 393 450 846 1158
Size: 221 172 57 396 312
Fragments arranged by size:
396 312 221 172 57
BbsI GAAGACnn'nnnn_
Cuts at: 0 137 294 1158
Size: 137 157 864
Fragments arranged by size:
864 157 137
BglI GCCn_nnn'nGGC
Cuts at: 0 58 225 850 1158
Size: 58 167 625 308
Fragments arranged by size:
625 308 167 58
BglII A'GATC_T
Cuts at: 0 657 764 1087 1158
Size: 657 107 323 71
Fragments arranged by size:
657 323 107 71
Bme1580I G_kGCm'C
Cuts at: 0 393 605 1158
Size: 393 212 553
Fragments arranged by size:
553 393 212
BmgBI CAC'GTC
Cuts at: 0 1083 1158
Size: 1083 75
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 1158
Size: 781 377
Bpu10I CC'TnA_GC
Cuts at: 0 1145 1158
Size: 1145 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 691 1158
Size: 511 180 467
Fragments arranged by size:
511 467 180
BsaAI yAC'GTr
Cuts at: 0 132 231 519 1158
Size: 132 99 288 639
Fragments arranged by size:
639 288 132 99
BsaBI GATnn'nnATC
Cuts at: 0 52 1158
Size: 52 1106
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 79 109 1158
Size: 79 30 1049
Fragments arranged by size:
1049 79 30
BseYI C'CCAG_C
Cuts at: 0 1068 1158
Size: 1068 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 386 486 1158
Size: 253 133 100 672
Fragments arranged by size:
672 253 133 100
BsiHKAI G_wGCw'C
Cuts at: 0 256 527 605 1158
Size: 256 271 78 553
Fragments arranged by size:
553 271 256 78
Bsp1286I G_dGCh'C
Cuts at: 0 221 256 393 450 527 605 846 1158
Size: 221 35 137 57 77 78 241 312
Fragments arranged by size:
312 241 221 137 78 77 57 35
BspHI T'CATG_A
Cuts at: 0 662 1158
Size: 662 496
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 414 557 599 729 821 1158
Size: 414 143 42 130 92 337
Fragments arranged by size:
414 337 143 130 92 42
BsrBI CCG'CTC
Cuts at: 0 506 1158
Size: 506 652
BsrGI T'GTAC_A
Cuts at: 0 77 1158
Size: 77 1081
BstAPI GCAn_nnn'nTGC
Cuts at: 0 600 1158
Size: 600 558
BstEII G'GTnAC_C
Cuts at: 0 946 1158
Size: 946 212
BstYI r'GATC_y
Cuts at: 0 285 657 764 1087 1119 1158
Size: 285 372 107 323 32 39
Fragments arranged by size:
372 323 285 107 39 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1067 1158
Size: 1067 91
BtsI GCAGTG_nn'
Cuts at: 0 450 1158
Size: 450 708
DraIII CAC_nnn'GTG
Cuts at: 0 522 1158
Size: 522 636
DrdI GACnn_nn'nnGTC
Cuts at: 0 319 780 1158
Size: 319 461 378
Fragments arranged by size:
461 378 319
EaeI y'GGCC_r
Cuts at: 0 50 1158
Size: 50 1108
EarI CTCTTCn'nnn_
Cuts at: 0 168 1158
Size: 168 990
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 889 1021 1158
Size: 781 108 132 137
Fragments arranged by size:
781 137 132 108
EcoO109I rG'GnC_Cy
Cuts at: 0 390 615 795 831 1158
Size: 390 225 180 36 327
Fragments arranged by size:
390 327 225 180 36
EcoRI G'AATT_C
Cuts at: 0 874 889 1158
Size: 874 15 269
Fragments arranged by size:
874 269 15
HaeII r_GCGC'y
Cuts at: 0 156 1005 1158
Size: 156 849 153
Fragments arranged by size:
849 156 153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 657 689 1111 1143 1158
Size: 657 32 422 32 15
Fragments arranged by size:
657 422 32 32 15
HincII GTy'rAC
Cuts at: 0 472 1158
Size: 472 686
MscI TGG'CCA
Cuts at: 0 52 1158
Size: 52 1106
MslI CAynn'nnrTG
Cuts at: 0 130 197 277 682 965 1158
Size: 130 67 80 405 283 193
Fragments arranged by size:
405 283 193 130 80 67
MspA1I CmG'CkG
Cuts at: 0 567 759 1158
Size: 567 192 399
Fragments arranged by size:
567 399 192
NspI r_CATG'y
Cuts at: 0 78 129 1158
Size: 78 51 1029
Fragments arranged by size:
1029 78 51
PciI A'CATG_T
Cuts at: 0 74 1158
Size: 74 1084
PfoI T'CCnGG_A
Cuts at: 0 611 1158
Size: 611 547
PmlI CAC'GTG
Cuts at: 0 132 231 519 1158
Size: 132 99 288 639
Fragments arranged by size:
639 288 132 99
PpuMI rG'GwC_Cy
Cuts at: 0 615 831 1158
Size: 615 216 327
Fragments arranged by size:
615 327 216
PspOMI G'GGCC_C
Cuts at: 0 389 1158
Size: 389 769
PspXI vC'TCGA_Gb
Cuts at: 0 839 1158
Size: 839 319
PstI C_TGCA'G
Cuts at: 0 457 554 803 1158
Size: 457 97 249 355
Fragments arranged by size:
457 355 249 97
PvuII CAG'CTG
Cuts at: 0 759 1158
Size: 759 399
SanDI GG'GwC_CC
Cuts at: 0 615 1158
Size: 615 543
SapI GCTCTTCn'nnn_
Cuts at: 0 168 1158
Size: 168 990
SbfI CC_TGCA'GG
Cuts at: 0 803 1158
Size: 803 355
SexAI A'CCwGG_T
Cuts at: 0 342 605 1158
Size: 342 263 553
Fragments arranged by size:
553 342 263
SfcI C'TryA_G
Cuts at: 0 453 510 550 799 1158
Size: 453 57 40 249 359
Fragments arranged by size:
453 359 249 57 40
SmaI CCC'GGG
Cuts at: 0 445 622 1158
Size: 445 177 536
Fragments arranged by size:
536 445 177
SmlI C'TyrA_G
Cuts at: 0 526 706 839 1158
Size: 526 180 133 319
Fragments arranged by size:
526 319 180 133
SphI G_CATG'C
Cuts at: 0 129 1158
Size: 129 1029
StuI AGG'CCT
Cuts at: 0 499 1158
Size: 499 659
StyI C'CwwG_G
Cuts at: 0 791 1158
Size: 791 367
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 209 903 934 1158
Size: 209 694 31 224
Fragments arranged by size:
694 224 209 31
TatI w'GTAC_w
Cuts at: 0 77 1158
Size: 77 1081
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1065 1158
Size: 1065 93
XhoI C'TCGA_G
Cuts at: 0 839 1158
Size: 839 319
XmaI C'CCGG_G
Cuts at: 0 443 620 1158
Size: 443 177 538
Fragments arranged by size:
538 443 177
Enzymes that do cut:
AarI AccI AcuI AflIII AlfI ApaI ApaLI ApoI
AvaI BanII BbsI BglI BglII Bme1580I BmgBI BpmI
Bpu10I BpuEI BsaAI BsaBI BsaXI BseYI BsgI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrGI BstAPI BstEII BstYI
BtgZI BtsI DraIII DrdI EaeI EarI Eco57MI EcoO109I
EcoRI HaeII Hin4I HincII MscI MslI MspA1I NspI
PciI PfoI PmlI PpuMI PspOMI PspXI PstI PvuII
SanDI SapI SbfI SexAI SfcI SmaI SmlI SphI
StuI StyI TaqII TatI XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI AlwNI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbvCI BcgI BciVI BclI BfrBI BlpI
BmrI BmtI BplI BsaI BsaHI BsaWI BseRI BsiEI
BsiWI BsmI BsmBI BspEI BsrDI BsrFI BssHII BssSI
BstBI BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraI
EagI EciI EcoICRI EcoNI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PmeI PpiI PshAI PsiI
PsrI PvuI RsrII SacI SacII SalI ScaI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
TstI Tth111I XbaI XmnI ZraI