################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BanI BbeI BbrPI BbvCI BcgI BciVI BcnI BglI BglII BlnI BpmI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspMI BsrBI BsrI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraII DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI Hin1I HincII HindII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluNI MroI MscI MunI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI TfiI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AcsI 328 AAATTC [GA]^AATT[CT] AflII 694 CTTAAG C^TTAAG AlwNI 439 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 328 AAATTC [GA]^AATT[CT] AspHI 75 GAGCTC G[AT]GC[AT]^C BamHI 3 GGATCC G^GATCC BanII 75 GAGCTC G[GA]GC[CT]^C BclI 321 TGATCA T^GATCA BfaI 884 CTAG C^TAG BfrI 694 CTTAAG C^TTAAG BlpI 77 GCTCAGC GC^T[ACGT]AGC BmyI 75 GAGCTC G[AGT]GC[ACT]^C Bpu1102I 77 GCTCAGC GC^T[ACGT]AGC BsaI 201 GAGACC GGTCTC(1/5) BsgI 305 GTGCAG GTGCAG(16/14) BsiEI 11 CGGCCG CG[GA][CT]^CG BsiHKAI 75 GAGCTC G[AT]GC[AT]^C BsmFI 248 GGGAC GGGAC(10/14) Bsp1286I 75 GAGCTC G[AGT]GC[ACT]^C Bsp1407I 897 TGTACA T^GTACA BspLU11I 621 ACATGT A^CATGT BsrGI 897 TGTACA T^GTACA BstXI 1043 CCAACTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 3 GGATCC [GA]^GATC[CT] CelII 77 GCTCAGC GC^T[ACGT]AGC Cfr13I 552 GGCCC G^G[ACGT]CC DraI 676 TTTAAA TTT^AAA DsaI 14 CCGTGG C^C[GA][CT]GG EaeI 11 CGGCCG [CT]^GGCC[GA] EagI 11 CGGCCG C^GGCCG EclXI 11 CGGCCG C^GGCCG Eco52I 11 CGGCCG C^GGCCG EcoNI 239 CCTTCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 321 TGATCA T^GATCA HaeII 142 AGCGCC [GA]GCGC^[CT] HindIII 166 AAGCTT A^AGCTT MaeI 884 CTAG C^TAG MflI 3 GGATCC [GA]^GATC[CT] MluI 53 ACGCGT A^CGCGT MspA1I 442 CAGCTG C[AC]G^C[GT]G NlaIV 3 GGATCC GG[ACGT]^[ACGT]CC PstI 138 CTGCAG CTGCA^G PvuII 442 CAGCTG CAG^CTG SacI 75 GAGCTC GAGCT^C Sau96I 552 GGCCC G^G[ACGT]CC ScaI 1080 AGTACT AGT^ACT SfcI 138 CTGCAG C^T[GA][CT]AG SmlI 694 CTTAAG C^T[CT][GA]AG SphI 888 GCATGC GCATG^C SspBI 897 TGTACA T^GTACA StuI 236 AGGCCT AGG^CCT TaqI 1069 TCGA T^CGA TthHB8I 1069 TCGA T^CGA XcmI 495 CCAGGAAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 3 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AflIII 53 ACGCGT A^C[GA][CT]GT 621 ACATGT BsmAI 201 GAGAC GTCTC(1/5) 878 GTCTC BsrDI 60 CATTGC GCAATG(2/0) 865 GCAATG BsrFI 9 GCCGGC [GA]^CCGG[CT] 67 GCCGGC BssSI 556 CACGAG CACGAG(-5/-1) 944 CTCGTG Cfr10I 9 GCCGGC [GA]^CCGG[CT] 67 GCCGGC EcoT14I 548 CCAAGG C^C[AT][AT]GG 1089 CCTTGG FauI 90 CCCGC CCCGC(4/6) 100 CCCGC HgaI 52 GACGC GACGC(5/10) 172 GACGC HphI 523 TCACC GGTGA(8/7) 1039 TCACC MaeIII 215 GTGAC ^GT[ACGT]AC 1038 GTCAC MslI 466 CATACCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 970 CATCTCAGTG NaeI 9 GCCGGC GCC^GGC 67 GCCGGC NgoMIV 9 GCCGGC G^CCGGC 67 GCCGGC SfaNI 1062 GATGC GCATC(5/9) 520 GCATC StyI 548 CCAAGG C^C[AT][AT]GG 1089 CCTTGG Tsp45I 215 GTGAC ^GT[GC]AC 1038 GTCAC Tsp509I 148 AATT ^AATT 329 AATT TspRI 457 CAGTG CA[GC]TG[ACGT][ACGT]^ 975 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AccII AciI AfaI AluI AlwI BbsI BbvI BpuAI BsaJI BsiYI BslI BssKI BstF5I BstNI BstUI Cac8I CfoI DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboI MboII MnlI MseI MspI MvaI MvnI MwoI NdeII NlaIII NspI PleI RsaI Sau3AI ScrFI Tru9I TseI