(Circular) (Six-base) MAPSORT of: pFN21AE1232.seq Check: 3339 from: 1 to: 5800 pFN21AE1232 5800 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3610 278 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3143 1937 Size: 1206 4594 Acc65I G'GTAC_C Cuts at: 3121 3121 Size: 5800 AclI AA'CG_TT Cuts at: 1563 3927 4300 1563 Size: 2364 373 3063 Fragments arranged by size: 3063 2364 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3863 5247 5515 1632 Size: 2231 1384 268 1917 Fragments arranged by size: 2231 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2699 820 Size: 197 1682 3921 Fragments arranged by size: 3921 1682 197 AflIII A'CryG_T Cuts at: 1203 2058 2626 4699 5676 1203 Size: 855 568 2073 977 1327 Fragments arranged by size: 2073 1327 977 855 568 AgeI A'CCGG_T Cuts at: 4631 4631 Size: 5800 AhdI GACnn_n'nnGTC Cuts at: 4529 4529 Size: 5800 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5800 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5800 AlwNI CAG_nnn'CTG Cuts at: 2449 3543 5115 2449 Size: 1094 1572 3134 Fragments arranged by size: 3134 1572 1094 ApaLI G'TGCA_C Cuts at: 3857 5013 3857 Size: 1156 4644 ApoI r'AATT_y Cuts at: 1493 2333 3109 3335 3487 5596 1493 Size: 840 776 226 152 2109 1697 Fragments arranged by size: 2109 1697 840 776 226 152 AseI AT'TA_AT Cuts at: 160 1032 4354 160 Size: 872 3322 1606 Fragments arranged by size: 3322 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 AvaI C'yCGr_G Cuts at: 1870 1943 3125 1870 Size: 73 1182 4545 Fragments arranged by size: 4545 1182 73 BamHI G'GATC_C Cuts at: 2008 3130 3497 2008 Size: 1122 367 4311 Fragments arranged by size: 4311 1122 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3121 4576 618 Size: 325 217 627 1334 1455 1842 Fragments arranged by size: 1842 1455 1334 627 325 217 BanII G_rGCy'C Cuts at: 1391 2084 3119 1391 Size: 693 1035 4072 Fragments arranged by size: 4072 1035 693 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2421 2432 3066 928 Size: 971 522 11 634 3662 Fragments arranged by size: 3662 971 634 522 11 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3991 4025 1903 Size: 34 2054 34 3678 Fragments arranged by size: 3678 2054 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3692 4902 3692 Size: 1210 4590 BclI T'GATC_A Cuts at: 1548 2326 1548 Size: 778 5022 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4411 136 Size: 107 122 71 631 605 2739 1525 Fragments arranged by size: 2739 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5795 5795 Size: 5800 BlpI GC'TnA_GC Cuts at: 2083 3217 2083 Size: 1134 4666 Bme1580I G_kGCm'C Cuts at: 1790 3861 5017 1790 Size: 2071 1156 2573 Fragments arranged by size: 2573 2071 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5800 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3531 4489 442 Size: 416 53 83 399 24 2114 958 1753 Fragments arranged by size: 2114 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5800 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2103 2103 Size: 5800 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4460 1239 Size: 3221 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3925 4790 5088 3925 Size: 865 298 4637 Fragments arranged by size: 4637 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2200 4463 882 Size: 617 701 2263 2219 Fragments arranged by size: 2263 2219 701 617 BsaAI yAC'GTr Cuts at: 493 1204 3591 493 Size: 711 2387 2702 Fragments arranged by size: 2702 2387 711 BsaBI GATnn'nnATC Cuts at: 5794 5794 Size: 5800 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3607 3989 4624 275 Size: 53 83 186 936 2074 382 635 1451 Fragments arranged by size: 2074 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4233 4631 4905 5052 5463 4233 Size: 398 274 147 411 4570 Fragments arranged by size: 4570 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5575 Fragments arranged by size: 5575 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5800 BseYI C'CCAG_C Cuts at: 2531 5003 5499 2531 Size: 2472 496 2832 Fragments arranged by size: 2832 2472 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2331 1163 Size: 573 595 4632 Fragments arranged by size: 4632 595 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2019 3250 4011 5039 1540 Size: 275 186 18 1231 761 1028 2301 Fragments arranged by size: 2301 1231 1028 761 275 186 18 BsiHKAI G_wGCw'C Cuts at: 2084 3861 3946 5017 2084 Size: 1777 85 1071 2867 Fragments arranged by size: 2867 1777 1071 85 BsmI GAATG_Cn' Cuts at: 3311 3404 3311 Size: 93 5707 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2084 3119 3861 3946 5017 1391 Size: 399 294 1035 742 85 1071 2174 Fragments arranged by size: 2174 1071 1035 742 399 294 85 BspEI T'CCGG_A Cuts at: 5463 5463 Size: 5800 BspHI T'CATG_A Cuts at: 3689 5419 3689 Size: 1730 4070 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3155 844 Size: 2311 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3687 1394 Size: 429 1864 3507 Fragments arranged by size: 3507 1864 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2064 2877 4295 4469 65 Size: 1183 816 813 1418 174 1396 Fragments arranged by size: 1418 1396 1183 816 813 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2014 2072 4444 4631 1700 Size: 110 204 58 2372 187 2869 Fragments arranged by size: 2869 2372 204 187 110 58 BsrGI T'GTAC_A Cuts at: 96 2902 96 Size: 2806 2994 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5800 BssSI C'ACGA_G Cuts at: 2561 2949 3860 4872 2561 Size: 388 911 1012 3489 Fragments arranged by size: 3489 1012 911 388 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5800 BstBI TT'CG_AA Cuts at: 4604 4604 Size: 5800 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5800 BstXI CCAn_nnnn'nTGG Cuts at: 3055 3055 Size: 5800 BstYI r'GATC_y Cuts at: 1972 2008 3130 3497 3882 3899 5351 5437 5449 Size: 36 1122 367 385 17 1452 86 12 Cuts at: 5449 5795 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1122 385 367 346 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2019 513 Size: 552 939 15 4294 Fragments arranged by size: 4294 939 552 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2035 487 Size: 683 56 809 4252 Fragments arranged by size: 4252 809 683 56 BtsI GCAGTG_nn' Cuts at: 3316 4110 4130 3316 Size: 794 20 4986 Fragments arranged by size: 4986 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5800 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5765 DraI TTT'AAA Cuts at: 2683 3104 2683 Size: 421 5379 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4807 809 Size: 507 3491 1802 Fragments arranged by size: 3491 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 3247 4136 Size: 54 1425 50 275 18 186 1231 889 Cuts at: 4136 5765 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1231 889 275 186 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2016 3247 1537 Size: 275 204 1231 4090 Fragments arranged by size: 4090 1231 275 204 EarI CTCTTCn'nnn_ Cuts at: 1353 3730 1353 Size: 2377 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2001 4387 4761 4907 387 Size: 1064 138 412 2386 374 146 1280 Fragments arranged by size: 2386 1280 1064 412 374 146 138 EcoICRI GAG'CTC Cuts at: 2082 2082 Size: 5800 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3863 4460 5247 5515 1239 Size: 393 2231 597 787 268 1524 Fragments arranged by size: 2231 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2248 2248 Size: 5800 EcoRI G'AATT_C Cuts at: 3109 3487 3109 Size: 378 5422 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2446 2714 2847 2446 Size: 268 133 5399 Fragments arranged by size: 5399 268 133 FspI TGC'GCA Cuts at: 4306 4306 Size: 5800 HaeII r_GCGC'y Cuts at: 1678 2151 4947 1678 Size: 473 2796 2531 Fragments arranged by size: 2796 2531 473 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2021 2053 4441 4473 4515 Size: 32 135 32 449 32 2388 32 42 Cuts at: 4515 4547 1373 Size: 32 2626 Fragments arranged by size: 2626 2388 449 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3144 3390 669 Size: 988 281 1206 246 3079 Fragments arranged by size: 3079 1206 988 281 246 HindIII A'AGCT_T Cuts at: 748 2171 748 Size: 1423 4377 HpaI GTT'AAC Cuts at: 3390 3390 Size: 5800 KpnI G_GTAC'C Cuts at: 3125 3125 Size: 5800 MfeI C'AATT_G Cuts at: 3399 3399 Size: 5800 MluI A'CGCG_T Cuts at: 2058 5676 2058 Size: 3618 2182 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2114 4914 5098 1305 Size: 809 2800 184 2007 Fragments arranged by size: 2800 2007 809 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3978 Fragments arranged by size: 3978 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2475 2979 3758 4117 4276 518 Size: 648 1309 504 779 359 159 2042 Fragments arranged by size: 2042 1309 779 648 504 359 159 MspA1I CmG'CkG Cuts at: 1606 2449 3216 3893 5041 5286 1606 Size: 843 767 677 1148 245 2120 Fragments arranged by size: 2120 1148 843 767 677 245 NaeI GCC'GGC Cuts at: 2016 2074 2016 Size: 58 5742 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4309 Fragments arranged by size: 4309 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5800 NgoMIV G'CCGG_C Cuts at: 2014 2072 2014 Size: 58 5742 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5800 NotI GC'GGCC_GC Cuts at: 3247 3247 Size: 5800 NspI r_CATG'y Cuts at: 1132 1229 2552 2630 2849 2897 3158 4703 1132 Size: 97 1323 78 219 48 261 1545 2229 Fragments arranged by size: 2229 1545 1323 261 219 97 78 48 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5800 PciI A'CATG_T Cuts at: 2626 4699 2626 Size: 2073 3727 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5800 PmeI GTTT'AAAC Cuts at: 3104 3104 Size: 5800 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3869 3901 5382 5414 3869 Size: 32 1481 32 4255 Fragments arranged by size: 4255 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5800 PsiI TTA'TAA Cuts at: 3370 3370 Size: 5800 PstI C_TGCA'G Cuts at: 830 2147 3152 830 Size: 1317 1005 3478 Fragments arranged by size: 3478 1317 1005 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5800 PvuII CAG'CTG Cuts at: 2449 2449 Size: 5800 SacI G_AGCT'C Cuts at: 2084 2084 Size: 5800 SalI G'TCGA_C Cuts at: 1936 3142 1936 Size: 1206 4594 SbfI CC_TGCA'GG Cuts at: 3152 3152 Size: 5800 ScaI AGT'ACT Cuts at: 3087 4048 5760 3087 Size: 961 1712 3127 Fragments arranged by size: 3127 1712 961 SfcI C'TryA_G Cuts at: 826 1045 2143 3148 4283 4964 5155 826 Size: 219 1098 1005 1135 681 191 1471 Fragments arranged by size: 1471 1135 1098 1005 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5800 SmaI CCC'GGG Cuts at: 1872 3127 1872 Size: 1255 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2699 3904 4805 5067 820 Size: 197 926 756 1205 901 262 1553 Fragments arranged by size: 1553 1205 926 901 756 262 197 SnaBI TAC'GTA Cuts at: 493 3591 493 Size: 3098 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5800 SphI G_CATG'C Cuts at: 1132 2897 3158 1132 Size: 1765 261 3774 Fragments arranged by size: 3774 1765 261 SspI AAT'ATT Cuts at: 5 52 3724 5 Size: 47 3672 2081 Fragments arranged by size: 3672 2081 47 StuI AGG'CCT Cuts at: 1517 2243 1517 Size: 726 5074 StyI C'CwwG_G Cuts at: 513 1065 2004 2553 3094 3239 513 Size: 552 939 549 541 145 3074 Fragments arranged by size: 3074 939 552 549 541 145 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2545 3061 3827 3844 3997 4182 4627 Size: 1255 965 516 766 17 153 185 445 Cuts at: 4627 325 Size: 1498 Fragments arranged by size: 1498 1255 965 766 516 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2902 3085 4046 Size: 274 80 33 51 1443 925 183 961 Cuts at: 4046 5758 96 Size: 1712 138 Fragments arranged by size: 1712 1443 961 925 274 183 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5760 5792 5760 Size: 32 5768 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5800 XbaI T'CTAG_A Cuts at: 3136 3136 Size: 5800 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2507 2507 Size: 5800 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5800 XmaI C'CCGG_G Cuts at: 1870 3125 1870 Size: 1255 4545 XmnI GAAnn'nnTTC Cuts at: 3929 3929 Size: 5800 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3608 276 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI