Restriction map for
pFN21AE1232
(Linear) (Six-base) MAPSORT of: KIEE1232.seq Check: 6901 from: 1 to: 1095
KIEE1232.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
AflII C'TTAA_G
Cuts at: 0 694 1095
Size: 694 401
AflIII A'CryG_T
Cuts at: 0 53 621 1095
Size: 53 568 474
Fragments arranged by size:
568 474 53
AlwNI CAG_nnn'CTG
Cuts at: 0 444 1095
Size: 444 651
ApoI r'AATT_y
Cuts at: 0 328 1095
Size: 328 767
BamHI G'GATC_C
Cuts at: 0 3 1095
Size: 3 1092
BanII G_rGCy'C
Cuts at: 0 79 1095
Size: 79 1016
BbsI GAAGACnn'nnnn_
Cuts at: 0 416 427 1061 1095
Size: 416 11 634 34
Fragments arranged by size:
634 416 34 11
BclI T'GATC_A
Cuts at: 0 321 1095
Size: 321 774
BlpI GC'TnA_GC
Cuts at: 0 78 1095
Size: 78 1017
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 98 1095
Size: 98 997
BsaI GGTCTCn'nnnn_
Cuts at: 0 195 1095
Size: 195 900
BseYI C'CCAG_C
Cuts at: 0 526 1095
Size: 526 569
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 1095
Size: 326 769
BsiEI CG_ry'CG
Cuts at: 0 14 1095
Size: 14 1081
BsiHKAI G_wGCw'C
Cuts at: 0 79 1095
Size: 79 1016
Bsp1286I G_dGCh'C
Cuts at: 0 79 1095
Size: 79 1016
BsrDI GCAATG_nn'
Cuts at: 0 59 872 1095
Size: 59 813 223
Fragments arranged by size:
813 223 59
BsrFI r'CCGG_y
Cuts at: 0 9 67 1095
Size: 9 58 1028
Fragments arranged by size:
1028 58 9
BsrGI T'GTAC_A
Cuts at: 0 897 1095
Size: 897 198
BssSI C'ACGA_G
Cuts at: 0 556 944 1095
Size: 556 388 151
Fragments arranged by size:
556 388 151
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1050 1095
Size: 1050 45
BstYI r'GATC_y
Cuts at: 0 3 1095
Size: 3 1092
BtgI C'CryG_G
Cuts at: 0 14 1095
Size: 14 1081
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 30 1095
Size: 30 1065
DraI TTT'AAA
Cuts at: 0 678 1095
Size: 678 417
EaeI y'GGCC_r
Cuts at: 0 11 1095
Size: 11 1084
EagI C'GGCC_G
Cuts at: 0 11 1095
Size: 11 1084
EcoICRI GAG'CTC
Cuts at: 0 77 1095
Size: 77 1018
EcoNI CCTnn'n_nnAGG
Cuts at: 0 243 1095
Size: 243 852
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 441 709 842 1095
Size: 441 268 133 253
Fragments arranged by size:
441 268 253 133
HaeII r_GCGC'y
Cuts at: 0 146 1095
Size: 146 949
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 16 48 1095
Size: 16 32 1047
Fragments arranged by size:
1047 32 16
HindIII A'AGCT_T
Cuts at: 0 166 1095
Size: 166 929
MluI A'CGCG_T
Cuts at: 0 53 1095
Size: 53 1042
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 1095
Size: 109 986
MslI CAynn'nnrTG
Cuts at: 0 470 974 1095
Size: 470 504 121
Fragments arranged by size:
504 470 121
MspA1I CmG'CkG
Cuts at: 0 444 1095
Size: 444 651
NaeI GCC'GGC
Cuts at: 0 11 69 1095
Size: 11 58 1026
Fragments arranged by size:
1026 58 11
NgoMIV G'CCGG_C
Cuts at: 0 9 67 1095
Size: 9 58 1028
Fragments arranged by size:
1028 58 9
NspI r_CATG'y
Cuts at: 0 547 625 844 892 1095
Size: 547 78 219 48 203
Fragments arranged by size:
547 219 203 78 48
PciI A'CATG_T
Cuts at: 0 621 1095
Size: 621 474
PstI C_TGCA'G
Cuts at: 0 142 1095
Size: 142 953
PvuII CAG'CTG
Cuts at: 0 444 1095
Size: 444 651
SacI G_AGCT'C
Cuts at: 0 79 1095
Size: 79 1016
ScaI AGT'ACT
Cuts at: 0 1082 1095
Size: 1082 13
SfcI C'TryA_G
Cuts at: 0 138 1095
Size: 138 957
SmlI C'TyrA_G
Cuts at: 0 694 1095
Size: 694 401
SphI G_CATG'C
Cuts at: 0 892 1095
Size: 892 203
StuI AGG'CCT
Cuts at: 0 238 1095
Size: 238 857
StyI C'CwwG_G
Cuts at: 0 548 1089 1095
Size: 548 541 6
Fragments arranged by size:
548 541 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 540 1056 1095
Size: 540 516 39
Fragments arranged by size:
540 516 39
TatI w'GTAC_w
Cuts at: 0 897 1080 1095
Size: 897 183 15
Fragments arranged by size:
897 183 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 502 1095
Size: 502 593
Enzymes that do cut:
AflII AflIII AlwNI ApoI BamHI BanII BbsI BclI
BlpI BplI BsaI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BsrDI BsrFI BsrGI BssSI BstXI BstYI BtgI BtgZI
DraI EaeI EagI EcoICRI EcoNI FalI HaeII Hin4I
HindIII MluI MmeI MslI MspA1I NaeI NgoMIV NspI
PciI PstI PvuII SacI ScaI SfcI SmlI SphI
StuI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BbvCI BcgI
BciVI BfrBI BglI BglII Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI
CspCI DraIII DrdI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FseI FspI FspAI HincII HpaI KasI KpnI
MfeI MscI NarI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI