Restriction map for
pFN21AE1219
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AfaI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstXI BstYI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoRV FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MflI MluI MluNI
MroI MscI MseI MslI MunI
NarI NdeI NheI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacII
SalI SapI SbfI ScaI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tth111I Van91I XbaI XcmI XhoI
XhoII
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 466 GTCTAC GT^[AC][GT]AC
AcsI 419 GAATTC [GA]^AATT[CT]
AlwNI 142 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 419 GAATTC [GA]^AATT[CT]
Asp700I 246 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BsgI 220 GTGCAG GTGCAG(16/14)
BsiEI 71 CGGCCG CG[GA][CT]^CG
BsmBI 645 GAGACG CGTCTC(1/5)
BstAPI 141 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 444 GGTGACC G^GT[ACGT]ACC
BstF5I 362 GGATG GGATG(2/0)
BstPI 444 GGTGACC G^GT[ACGT]ACC
DsaI 440 CCATGG C^C[GA][CT]GG
EagI 71 CGGCCG C^GGCCG
EarI 424 CTCTTC CTCTTC(1/4)
EclXI 71 CGGCCG C^GGCCG
Eco52I 71 CGGCCG C^GGCCG
EcoNI 531 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 444 GGTGACC G^GT[ACGT]ACC
EcoRI 419 GAATTC G^AATTC
EcoT14I 440 CCATGG C^C[AT][AT]GG
EcoT22I 304 ATGCAT ATGCA^T
FokI 362 GGATG GGATG(9/13)
HgaI 464 GCGTC GACGC(5/10)
Ksp632I 424 CTCTTC CTCTTC(1/4)
MaeII 266 ACGT A^CGT
MboII 425 TCTTC GAAGA(8/7)
MspA1I 238 CCGCTG C[AC]G^C[GT]G
NcoI 440 CCATGG C^CATGG
NotI 70 GCGGCCGC GC^GGCCGC
NsiI 304 ATGCAT ATGCA^T
PpuMI 143 AGGACCT [GA]G^G[AT]CC[CT]
SexAI 536 ACCAGGT A^CC[AT]GGT
SmaI 132 CCCGGG CCC^GGG
StyI 440 CCATGG C^C[AT][AT]GG
TaqI 283 TCGA T^CGA
Tsp509I 420 AATT ^AATT
TspRI 414 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 283 TCGA T^CGA
XmaCI 132 CCCGGG C^CCGGG
XmaI 132 CCCGGG C^CCGGG
XmnI 246 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 30 CGCG CG^CG
631 CGCG
Alw44I 7 GTGCAC G^TGCAC
231 GTGCAC
ApaLI 7 GTGCAC G^TGCAC
231 GTGCAC
AvaI 132 CCCGGG C^[CT]CG[GA]G
175 CCCGAG
BglI 25 GCCGGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
613 GCCTTCCTGGC
BpmI 118 CTGGAG CTGGAG(16/14)
259 CTGGAG
BsmAI 645 GAGAC GTCTC(1/5)
509 GTCTC
BsmFI 310 GGGAC GGGAC(10/14)
561 GTCCC
BsoBI 132 CCCGGG C^[CT]CG[GA]G
175 CCCGAG
BspMI 146 ACCTGC ACCTGC(4/8)
211 ACCTGC
BsrBI 331 CCGCTC CCGCTC(-3/-3)
112 GAGCGG
BsrFI 25 GCCGGC [GA]^CCGG[CT]
287 GCCGGC
BsrI 127 ACTGG ACTGG(1/-1)
415 ACTGG
BstUI 30 CGCG CG^CG
631 CGCG
Cfr10I 25 GCCGGC [GA]^CCGG[CT]
287 GCCGGC
DraII 143 AGGACCT [GA]G^G[ACGT]CC[CT]
524 AGGCCCT
EaeI 23 TGGCCG [CT]^GGCC[GA]
71 CGGCCG
EcoO109I 143 AGGACCT [GA]G^G[ACGT]CC[CT]
524 AGGCCCT
HaeII 48 GGCGCT [GA]GCGC^[CT]
501 GGCGCT
HinfI 280 GAATC G^A[ACGT]TC
321 GATTC
MaeIII 268 GTGAC ^GT[ACGT]AC
445 GTGAC
MvnI 30 CGCG CG^CG
631 CGCG
NaeI 25 GCCGGC GCC^GGC
287 GCCGGC
NgoMIV 25 GCCGGC G^CCGGC
287 GCCGGC
SacI 186 GAGCTC GAGCT^C
634 GAGCTC
TfiI 280 GAATC G^A[AT]TC
321 GATTC
Tsp45I 268 GTGAC ^GT[GC]AC
445 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BanII BbvI BcnI BlpI BmyI
Bpu1102I BsaJI BseRI BsiHKAI BsiYI
BslI Bsp1286I BssKI BstNI Cac8I
CelII CfoI Cfr13I DdeI DpnII
EcoRII FauI Fnu4HI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MboI MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI TseI