(Circular) (Six-base) MAPSORT of: pFN21AE1219.seq Check: 8646 from: 1 to: 5374 pFN21AE1219 5374 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3184 278 Size: 53 83 186 936 1648 2468 Fragments arranged by size: 2468 1648 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2472 2717 1937 Size: 535 245 4594 Fragments arranged by size: 4594 535 245 Acc65I G'GTAC_C Cuts at: 2695 2695 Size: 5374 AclI AA'CG_TT Cuts at: 1563 3501 3874 1563 Size: 1938 373 3063 Fragments arranged by size: 3063 1938 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2242 3437 4821 5089 1632 Size: 610 1195 1384 268 1917 Fragments arranged by size: 1917 1384 1195 610 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5177 AflIII A'CryG_T Cuts at: 1203 4273 5250 1203 Size: 3070 977 1327 Fragments arranged by size: 3070 1327 977 AgeI A'CCGG_T Cuts at: 4205 4205 Size: 5374 AhdI GACnn_n'nnGTC Cuts at: 4103 4103 Size: 5374 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5374 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5374 AlwNI CAG_nnn'CTG Cuts at: 2152 3117 4689 2152 Size: 965 1572 2837 Fragments arranged by size: 2837 1572 965 ApaLI G'TGCA_C Cuts at: 2012 2236 3431 4587 2012 Size: 224 1195 1156 2799 Fragments arranged by size: 2799 1195 1156 224 ApoI r'AATT_y Cuts at: 1493 2424 2683 2909 3061 5170 1493 Size: 931 259 226 152 2109 1697 Fragments arranged by size: 2109 1697 931 259 226 152 AseI AT'TA_AT Cuts at: 160 1032 3928 160 Size: 872 2896 1606 Fragments arranged by size: 2896 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5374 AvaI C'yCGr_G Cuts at: 1870 1943 2137 2180 2699 1870 Size: 73 194 43 519 4545 Fragments arranged by size: 4545 519 194 73 43 BamHI G'GATC_C Cuts at: 2704 3071 2704 Size: 367 5007 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2695 4150 618 Size: 325 217 627 908 1455 1842 Fragments arranged by size: 1842 1455 908 627 325 217 BanII G_rGCy'C Cuts at: 1391 2195 2234 2643 2693 1391 Size: 804 39 409 50 4072 Fragments arranged by size: 4072 804 409 50 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4403 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3565 3599 1903 Size: 34 50 34 1544 34 3678 Fragments arranged by size: 3678 1544 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3266 4476 3266 Size: 1210 4164 BclI T'GATC_A Cuts at: 1548 1548 Size: 5374 BfrBI ATG'CAT Cuts at: 2311 2311 Size: 5374 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2036 2624 3985 Size: 107 122 71 631 605 364 588 1361 Cuts at: 3985 136 Size: 1525 Fragments arranged by size: 1525 1361 631 605 588 364 122 107 71 BglII A'GATC_T Cuts at: 5369 5369 Size: 5374 BlpI GC'TnA_GC Cuts at: 2057 2339 2642 2791 2057 Size: 282 303 149 4640 Fragments arranged by size: 4640 303 282 149 Bme1580I G_kGCm'C Cuts at: 1790 2016 2240 3435 4591 1790 Size: 226 224 1195 1156 2573 Fragments arranged by size: 2573 1195 1156 226 224 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5374 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3105 4063 442 Size: 416 53 83 399 24 1688 958 1753 Fragments arranged by size: 1753 1688 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5374 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2144 2285 4034 1239 Size: 905 141 1749 2579 Fragments arranged by size: 2579 1749 905 141 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3499 4364 4662 3499 Size: 865 298 4211 Fragments arranged by size: 4211 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4037 882 Size: 617 2538 2219 Fragments arranged by size: 2538 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3165 493 Size: 711 1961 2702 Fragments arranged by size: 2702 1961 711 BsaBI GATnn'nnATC Cuts at: 5368 5368 Size: 5374 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3181 3563 4198 275 Size: 53 83 186 936 1648 382 635 1451 Fragments arranged by size: 1648 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3807 4205 4479 4626 5037 3807 Size: 398 274 147 411 4144 Fragments arranged by size: 4144 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2433 2463 2495 2525 1178 Size: 30 165 30 1030 30 32 30 4027 Fragments arranged by size: 4027 1030 165 32 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2381 2396 2399 2537 1186 Size: 1195 15 3 138 4023 Fragments arranged by size: 4023 1195 138 15 3 BseYI C'CCAG_C Cuts at: 2155 2459 4577 5073 2155 Size: 304 2118 496 2456 Fragments arranged by size: 2456 2118 496 304 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2246 1163 Size: 573 510 4291 Fragments arranged by size: 4291 573 510 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2079 2824 3585 4613 1540 Size: 275 186 78 745 761 1028 2301 Fragments arranged by size: 2301 1028 761 745 275 186 78 BsiHKAI G_wGCw'C Cuts at: 2016 2070 2195 2240 2643 3435 3520 4591 2016 Size: 54 125 45 403 792 85 1071 2799 Fragments arranged by size: 2799 1071 792 403 125 85 54 45 BsmI GAATG_Cn' Cuts at: 2885 2978 2885 Size: 93 5281 BsmBI CGTCTCn'nnnn_ Cuts at: 2644 2644 Size: 5374 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2070 2195 2234 2240 2643 2693 Size: 399 226 54 125 39 6 403 50 Cuts at: 2693 3435 3520 4591 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 403 399 226 125 85 54 50 39 6 BspEI T'CCGG_A Cuts at: 5037 5037 Size: 5374 BspHI T'CATG_A Cuts at: 3263 4993 3263 Size: 1730 3644 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2160 2225 2729 844 Size: 1316 65 504 3489 Fragments arranged by size: 3489 1316 504 65 BsrBI CCG'CTC Cuts at: 1394 1823 2119 2338 3261 1394 Size: 429 296 219 923 3507 Fragments arranged by size: 3507 923 429 296 219 BsrDI GCAATG_nn' Cuts at: 65 1248 3869 4043 65 Size: 1183 2621 174 1396 Fragments arranged by size: 2621 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2030 2292 4018 4205 1700 Size: 110 220 262 1726 187 2869 Fragments arranged by size: 2869 1726 262 220 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5374 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5374 BssSI C'ACGA_G Cuts at: 3434 4446 3434 Size: 1012 4362 BstAPI GCAn_nnn'nTGC Cuts at: 794 2152 794 Size: 1358 4016 BstBI TT'CG_AA Cuts at: 4178 4178 Size: 5374 BstEII G'GTnAC_C Cuts at: 1184 2449 1184 Size: 1265 4109 BstYI r'GATC_y Cuts at: 1972 2704 3071 3456 3473 4925 5011 5023 5369 Size: 732 367 385 17 1452 86 12 346 Cuts at: 5369 1972 Size: 1977 Fragments arranged by size: 1977 1452 732 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2445 513 Size: 552 1380 3442 Fragments arranged by size: 3442 1380 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2578 487 Size: 683 56 1352 3283 Fragments arranged by size: 3283 1352 683 56 BtsI GCAGTG_nn' Cuts at: 2890 3684 3704 2890 Size: 794 20 4560 Fragments arranged by size: 4560 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5374 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5339 DraI TTT'AAA Cuts at: 2678 2678 Size: 5374 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4381 809 Size: 507 3065 1802 Fragments arranged by size: 3065 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2076 2821 Size: 54 1425 50 275 18 198 48 745 Cuts at: 2821 3710 5339 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 745 275 198 54 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2076 2821 1537 Size: 275 264 745 4090 Fragments arranged by size: 4090 745 275 264 EarI CTCTTCn'nnn_ Cuts at: 1353 2435 3304 1353 Size: 1082 869 3423 Fragments arranged by size: 3423 1082 869 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2227 3961 4335 4481 387 Size: 1064 138 638 1734 374 146 1280 Fragments arranged by size: 1734 1280 1064 638 374 146 138 EcoICRI GAG'CTC Cuts at: 2193 2641 2193 Size: 448 4926 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 2242 2285 3437 4034 4821 5089 Size: 393 512 98 43 1152 597 787 268 Cuts at: 5089 1239 Size: 1524 Fragments arranged by size: 1524 1152 787 597 512 393 268 98 43 EcoNI CCTnn'n_nnAGG Cuts at: 2540 2540 Size: 5374 EcoO109I rG'GnC_Cy Cuts at: 2149 2530 2149 Size: 381 4993 EcoRI G'AATT_C Cuts at: 2424 2683 3061 2424 Size: 259 378 4737 Fragments arranged by size: 4737 378 259 FspI TGC'GCA Cuts at: 3880 3880 Size: 5374 HaeII r_GCGC'y Cuts at: 1678 2057 2510 4521 1678 Size: 379 453 2011 2531 Fragments arranged by size: 2531 2011 453 379 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4015 4047 4089 4121 1373 Size: 32 135 32 2443 32 42 32 2626 Fragments arranged by size: 2626 2443 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2718 2964 669 Size: 988 281 780 246 3079 Fragments arranged by size: 3079 988 780 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5374 HpaI GTT'AAC Cuts at: 2964 2964 Size: 5374 KpnI G_GTAC'C Cuts at: 2699 2699 Size: 5374 MfeI C'AATT_G Cuts at: 2973 2973 Size: 5374 MluI A'CGCG_T Cuts at: 5250 5250 Size: 5374 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4488 4672 1305 Size: 3183 184 2007 Fragments arranged by size: 3183 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3552 Fragments arranged by size: 3552 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3332 3691 3850 518 Size: 648 843 1323 359 159 2042 Fragments arranged by size: 2042 1323 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2245 2790 3467 4615 4860 1606 Size: 639 545 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 639 545 245 NaeI GCC'GGC Cuts at: 2032 2294 2032 Size: 262 5112 NcoI C'CATG_G Cuts at: 513 1065 2445 513 Size: 552 1380 3442 Fragments arranged by size: 3442 1380 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5374 NgoMIV G'CCGG_C Cuts at: 2030 2292 2030 Size: 262 5112 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5374 NotI GC'GGCC_GC Cuts at: 2076 2821 2076 Size: 745 4629 NsiI A_TGCA'T Cuts at: 2313 2313 Size: 5374 NspI r_CATG'y Cuts at: 1132 1229 2732 4277 1132 Size: 97 1503 1545 2229 Fragments arranged by size: 2229 1545 1503 97 PasI CC'CwG_GG Cuts at: 1348 2575 1348 Size: 1227 4147 PciI A'CATG_T Cuts at: 4273 4273 Size: 5374 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5374 PfoI T'CCnGG_A Cuts at: 2499 2535 2499 Size: 36 5338 PmeI GTTT'AAAC Cuts at: 2678 2678 Size: 5374 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3443 3475 4956 4988 3443 Size: 32 1481 32 3829 Fragments arranged by size: 3829 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2149 2149 Size: 5374 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5374 PsiI TTA'TAA Cuts at: 2944 2944 Size: 5374 PstI C_TGCA'G Cuts at: 830 2726 830 Size: 1896 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5374 SacI G_AGCT'C Cuts at: 2195 2643 2195 Size: 448 4926 SalI G'TCGA_C Cuts at: 1936 2716 1936 Size: 780 4594 SbfI CC_TGCA'GG Cuts at: 2726 2726 Size: 5374 ScaI AGT'ACT Cuts at: 3622 5334 3622 Size: 1712 3662 SexAI A'CCwGG_T Cuts at: 2541 2541 Size: 5374 SfcI C'TryA_G Cuts at: 826 1045 2722 3857 4538 4729 826 Size: 219 1677 1135 681 191 1471 Fragments arranged by size: 1677 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5374 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5374 SmaI CCC'GGG Cuts at: 1872 2139 2701 1872 Size: 267 562 4545 Fragments arranged by size: 4545 562 267 SmlI C'TyrA_G Cuts at: 820 1017 1943 3478 4379 4641 820 Size: 197 926 1535 901 262 1553 Fragments arranged by size: 1553 1535 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3165 493 Size: 2672 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5374 SphI G_CATG'C Cuts at: 1132 2732 1132 Size: 1600 3774 SspI AAT'ATT Cuts at: 5 52 3298 5 Size: 47 3246 2081 Fragments arranged by size: 3246 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5374 StyI C'CwwG_G Cuts at: 513 1065 2445 2813 513 Size: 552 1380 368 3074 Fragments arranged by size: 3074 1380 552 368 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3401 3418 3571 3756 4201 325 Size: 1255 1821 17 153 185 445 1498 Fragments arranged by size: 1821 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3620 5332 96 Size: 274 80 33 51 1443 1643 1712 138 Fragments arranged by size: 1712 1643 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2433 2465 2495 2527 5334 5366 2433 Size: 32 30 32 2807 32 2441 Fragments arranged by size: 2807 2441 32 32 32 30 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5374 XbaI T'CTAG_A Cuts at: 2710 2710 Size: 5374 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5374 XmaI C'CCGG_G Cuts at: 1870 2137 2699 1870 Size: 267 562 4545 Fragments arranged by size: 4545 562 267 XmnI GAAnn'nnTTC Cuts at: 2255 3503 2255 Size: 1248 4126 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3182 276 Size: 53 83 186 936 1648 2468 Fragments arranged by size: 2468 1648 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI