(Linear) (Six-base) MAPSORT of: KIEE1219.seq Check: 1833 from: 1 to: 669 KIEE1219.orf With 182 enzymes: SgfI * July 15, 2010 18:35 .. AccI GT'mk_AC Cuts at: 0 467 669 Size: 467 202 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 237 669 Size: 237 432 AlwNI CAG_nnn'CTG Cuts at: 0 147 669 Size: 147 522 ApaLI G'TGCA_C Cuts at: 0 7 231 669 Size: 7 224 438 Fragments arranged by size: 438 224 7 ApoI r'AATT_y Cuts at: 0 419 669 Size: 419 250 AvaI C'yCGr_G Cuts at: 0 132 175 669 Size: 132 43 494 Fragments arranged by size: 494 132 43 BanII G_rGCy'C Cuts at: 0 190 229 638 669 Size: 190 39 409 31 Fragments arranged by size: 409 190 39 31 BfrBI ATG'CAT Cuts at: 0 306 669 Size: 306 363 BglI GCCn_nnn'nGGC Cuts at: 0 31 619 669 Size: 31 588 50 Fragments arranged by size: 588 50 31 BlpI GC'TnA_GC Cuts at: 0 52 334 637 669 Size: 52 282 303 32 Fragments arranged by size: 303 282 52 32 Bme1580I G_kGCm'C Cuts at: 0 11 235 669 Size: 11 224 434 Fragments arranged by size: 434 224 11 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 139 280 669 Size: 139 141 389 Fragments arranged by size: 389 141 139 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 428 458 490 520 669 Size: 428 30 32 30 149 Fragments arranged by size: 428 149 32 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 376 391 394 532 669 Size: 376 15 3 138 137 Fragments arranged by size: 376 138 137 15 3 BseYI C'CCAG_C Cuts at: 0 150 454 669 Size: 150 304 215 Fragments arranged by size: 304 215 150 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 241 669 Size: 241 428 BsiEI CG_ry'CG Cuts at: 0 74 669 Size: 74 595 BsiHKAI G_wGCw'C Cuts at: 0 11 65 190 235 638 669 Size: 11 54 125 45 403 31 Fragments arranged by size: 403 125 54 45 31 11 BsmBI CGTCTCn'nnnn_ Cuts at: 0 639 669 Size: 639 30 Bsp1286I G_dGCh'C Cuts at: 0 11 65 190 229 235 638 669 Size: 11 54 125 39 6 403 31 Fragments arranged by size: 403 125 54 39 31 11 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 155 220 669 Size: 155 65 449 Fragments arranged by size: 449 155 65 BsrBI CCG'CTC Cuts at: 0 114 333 669 Size: 114 219 336 Fragments arranged by size: 336 219 114 BsrFI r'CCGG_y Cuts at: 0 25 287 669 Size: 25 262 382 Fragments arranged by size: 382 262 25 BstAPI GCAn_nnn'nTGC Cuts at: 0 147 669 Size: 147 522 BstEII G'GTnAC_C Cuts at: 0 444 669 Size: 444 225 BtgI C'CryG_G Cuts at: 0 440 669 Size: 440 229 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 573 669 Size: 573 96 EaeI y'GGCC_r Cuts at: 0 23 71 669 Size: 23 48 598 Fragments arranged by size: 598 48 23 EagI C'GGCC_G Cuts at: 0 71 669 Size: 71 598 EarI CTCTTCn'nnn_ Cuts at: 0 430 669 Size: 430 239 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 222 669 Size: 222 447 EcoICRI GAG'CTC Cuts at: 0 188 636 669 Size: 188 448 33 Fragments arranged by size: 448 188 33 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 139 237 280 669 Size: 139 98 43 389 Fragments arranged by size: 389 139 98 43 EcoNI CCTnn'n_nnAGG Cuts at: 0 535 669 Size: 535 134 EcoO109I rG'GnC_Cy Cuts at: 0 144 525 669 Size: 144 381 144 Fragments arranged by size: 381 144 144 EcoRI G'AATT_C Cuts at: 0 419 669 Size: 419 250 HaeII r_GCGC'y Cuts at: 0 52 505 669 Size: 52 453 164 Fragments arranged by size: 453 164 52 MspA1I CmG'CkG Cuts at: 0 240 669 Size: 240 429 NaeI GCC'GGC Cuts at: 0 27 289 669 Size: 27 262 380 Fragments arranged by size: 380 262 27 NcoI C'CATG_G Cuts at: 0 440 669 Size: 440 229 NgoMIV G'CCGG_C Cuts at: 0 25 287 669 Size: 25 262 382 Fragments arranged by size: 382 262 25 NotI GC'GGCC_GC Cuts at: 0 71 669 Size: 71 598 NsiI A_TGCA'T Cuts at: 0 308 669 Size: 308 361 PasI CC'CwG_GG Cuts at: 0 570 669 Size: 570 99 PfoI T'CCnGG_A Cuts at: 0 494 530 669 Size: 494 36 139 Fragments arranged by size: 494 139 36 PpuMI rG'GwC_Cy Cuts at: 0 144 669 Size: 144 525 SacI G_AGCT'C Cuts at: 0 190 638 669 Size: 190 448 31 Fragments arranged by size: 448 190 31 SexAI A'CCwGG_T Cuts at: 0 536 669 Size: 536 133 SmaI CCC'GGG Cuts at: 0 134 669 Size: 134 535 StyI C'CwwG_G Cuts at: 0 440 669 Size: 440 229 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 428 460 490 522 669 Size: 428 32 30 32 147 Fragments arranged by size: 428 147 32 32 30 XmaI C'CCGG_G Cuts at: 0 132 669 Size: 132 537 XmnI GAAnn'nnTTC Cuts at: 0 250 669 Size: 250 419 Enzymes that do cut: AccI AcuI AlwNI ApaLI ApoI AvaI BanII BfrBI BglI BlpI Bme1580I BpmI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BstAPI BstEII BtgI BtgZI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII MspA1I NaeI NcoI NgoMIV NotI NsiI PasI PfoI PpuMI SacI SexAI SmaI StyI TstI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI BglII BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI BstXI BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI NarI NdeI NheI NruI NspI PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI XcmI XhoI ZraI