################################## # NO CUT ENZYMES ( 176 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeIII MboI MfeI MflI MluI MluNI MscI MseI MunI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccII 85 CGCG CG^CG AccIII 52 TCCGGA T^CCGGA ApaI 59 GGGCCC GGGCC^C AspHI 149 GTGCTC G[AT]GC[AT]^C AvaII 104 GGACC G^G[AT]CC BciVI 286 GGATAC GTATCC(6/5) BfaI 46 CTAG C^TAG BglI 172 GCCTTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 214 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 18 GGTCTC GGTCTC(1/5) BsaWI 52 TCCGGA [AT]^CCGG[AT] BseAI 52 TCCGGA T^CCGGA BseRI 88 GAGGAG GAGGAG(10/8) BsiHKAI 149 GTGCTC G[AT]GC[AT]^C BsmAI 19 GTCTC GTCTC(1/5) BsmFI 103 GGGAC GGGAC(10/14) BspEI 52 TCCGGA T^CCGGA BsrI 292 ACTGG ACTGG(1/-1) BstUI 85 CGCG CG^CG BstXI 263 CCACGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DdeI 194 CTGAG C^T[ACGT]AG DraII 251 GGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 14 CCACGG C^C[GA][CT]GG EarI 67 CTCTTC CTCTTC(1/4) EcoO109I 251 GGGGCCT [GA]G^G[ACGT]CC[CT] FauI 249 GCGGG CCCGC(4/6) HinfI 279 GAGTC G^A[ACGT]TC Ksp632I 67 CTCTTC CTCTTC(1/4) MaeI 46 CTAG C^TAG MaeII 74 ACGT A^CGT MamI 214 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 52 TCCGGA T^CCGGA MslI 126 CATCCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 85 CGCG CG^CG NruI 84 TCGCGA TCG^CGA PleI 279 GAGTC GAGTC(4/5) PstI 154 CTGCAG CTGCA^G SapI 66 GCTCTTC GCTCTTC(1/4) SfaNI 219 GCATC GCATC(5/9) TaqI 71 TCGA T^CGA TspRI 291 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 71 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### AciI 94 GCGG CCGC(-3/-1) 249 GCGG BanII 59 GGGCCC G[GA]GC[CT]^C 296 GGGCTC BsiYI 50 CCTCCGGACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 107 CCATCCCAGGG BslI 50 CCTCCGGACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 107 CCATCCCAGGG BstF5I 108 CATCC GGATG(2/0) 126 CATCC FokI 108 CATCC GGATG(9/13) 126 CATCC HaeII 9 AGCGCC [GA]GCGC^[CT] 117 GGCGCT SfcI 154 CTGCAG C^T[GA][CT]AG 318 CTATAG ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AluI BbvI BcnI BmyI BpmI BsaJI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MboII MnlI MspA1I MspI MvaI MwoI NciI NlaIV PvuII Sau96I ScrFI TseI