Restriction map for
pFN21AE2058
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII AclI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspHI AspI AviII
AvrII BbeI BbrPI BbvCI BclI
BfrI BglII BlnI BsaBI BsaHI
BsiHKAI BsiWI BsmBI Bsp1407I BspDI
BspLU11I BsrBI BsrGI BssHII Bst1107I
BstAPI BstBI BstZ17I Bsu36I ClaI
CpoI DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRV EcoT22I FbaI FseI FspI
Hin1I KasI Ksp632I KspI MamI
MfeI MluI MunI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 1753 GGTACC G^GTACC
AccII 124 CGCG CG^CG
AccIII 1025 TCCGGA T^CCGGA
Asp718I 1753 GGTACC G^GTACC
BamHI 324 GGATCC G^GATCC
BbsI 1180 GAAGAC GAAGAC(2/6)
BcgI 222 CGAAGCGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 749 CCCGG CC^[GC]GG
BfaI 421 CTAG C^TAG
BglI 208 GCCATTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1095 GCTGAGC GC^T[ACGT]AGC
BpmI 1530 CTCCAG CTGGAG(16/14)
Bpu1102I 1095 GCTGAGC GC^T[ACGT]AGC
BpuAI 1180 GAAGAC GAAGAC(2/6)
BsaAI 482 CACGTA [CT]AC^GT[GA]
BsaI 1171 GAGACC GGTCTC(1/5)
BseAI 1025 TCCGGA T^CCGGA
BsiEI 1924 CGGTCG CG[GA][CT]^CG
BsmI 1069 GAATGC GAATGC(1/-1)
BspEI 1025 TCCGGA T^CCGGA
BsrDI 210 CATTGC GCAATG(2/0)
BsrFI 756 GCCGGT [GA]^CCGG[CT]
BssSI 1105 CACGAG CACGAG(-5/-1)
BstEII 2035 GGTAACC G^GT[ACGT]ACC
BstPI 2035 GGTAACC G^GT[ACGT]ACC
BstUI 124 CGCG CG^CG
BstXI 1220 CCATGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 324 GGATCC [GA]^GATC[CT]
CelII 1095 GCTGAGC GC^T[ACGT]AGC
Cfr10I 756 GCCGGT [GA]^CCGG[CT]
DraI 963 TTTAAA TTT^AAA
EcoO65I 2035 GGTAACC G^GT[ACGT]ACC
EcoRI 898 GAATTC G^AATTC
FauI 1252 GCGGG CCCGC(4/6)
HaeII 1612 GGCGCT [GA]GCGC^[CT]
HindIII 1838 AAGCTT A^AGCTT
HpaI 1135 GTTAAC GTT^AAC
KpnI 1753 GGTACC GGTAC^C
MaeI 421 CTAG C^TAG
MflI 324 GGATCC [GA]^GATC[CT]
MroI 1025 TCCGGA T^CCGGA
MslI 1440 CATCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 124 CGCG CG^CG
NciI 749 CCCGG CC^[GC]GG
NspI 1045 ACATGC [GA]CATG^[CT]
PshAI 1528 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1476 CTGCAG CTGCA^G
StuI 1560 AGGCCT AGG^CCT
XhoII 324 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 1243 GTCTAC GT^[AC][GT]AC
2087 GTAGAC
AcsI 898 GAATTC [GA]^AATT[CT]
1489 GAATTT
AfaI 996 GTAC GT^AC
1754 GTAC
AlwNI 1361 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1533 CAGTCTCTG
ApoI 898 GAATTC [GA]^AATT[CT]
1489 GAATTT
AvaI 519 CTCGGG C^[CT]CG[GA]G
1902 CTCGGG
BanI 1269 GGTGCC G^G[CT][GA]CC
1753 GGTACC
BciVI 1314 GGATAC GTATCC(6/5)
1594 GGATAC
BsaWI 1025 TCCGGA [AT]^CCGG[AT]
1922 TCCGGT
BseRI 917 CTCCTC GAGGAG(10/8)
1771 GAGGAG
BsgI 2076 CTGCAC GTGCAG(16/14)
83 GTGCAG
BsmAI 1171 GAGAC GTCTC(1/5)
1535 GTCTC
BsoBI 519 CTCGGG C^[CT]CG[GA]G
1902 CTCGGG
BspHI 1199 TCATGA T^CATGA
1980 TCATGA
DraIII 105 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1810 CACGCTGTG
HgaI 140 GCGTC GACGC(5/10)
1844 GCGTC
NcoI 1445 CCATGG C^CATGG
1702 CCATGG
NdeI 130 CATATG CA^TATG
1994 CATATG
PflMI 148 CCATCCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
580 CCAGGGTGTGG
PleI 946 GACTC GAGTC(4/5)
1528 GACTC
PvuII 525 CAGCTG CAG^CTG
562 CAGCTG
RcaI 1199 TCATGA T^CATGA
1980 TCATGA
RsaI 996 GTAC GT^AC
1754 GTAC
SfcI 1340 CTGTAG C^T[GA][CT]AG
1476 CTGCAG
SmlI 493 CTTGAG C^T[CT][GA]AG
1114 CTCAAG
TaqI 393 TCGA T^CGA
679 TCGA
TthHB8I 393 TCGA T^CGA
679 TCGA
Van91I 148 CCATCCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
580 CCAGGGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AluI AlwI AvaII BalI
BanII BbvI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PpuMI Sau3AI Sau96I ScrFI
SfaNI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI