(Circular) (Six-base) MAPSORT of: pFN21AE2058.seq Check: 6299 from: 1 to: 6799 pFN21AE2058 6799 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4609 278 Size: 53 83 186 936 3073 2468 Fragments arranged by size: 3073 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3249 4093 4142 1937 Size: 1312 844 49 4594 Fragments arranged by size: 4594 1312 844 49 Acc65I G'GTAC_C Cuts at: 3758 4120 3758 Size: 362 6437 AclI AA'CG_TT Cuts at: 1563 4926 5299 1563 Size: 3363 373 3063 Fragments arranged by size: 3363 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2312 2666 4862 6246 6514 1632 Size: 680 354 2196 1384 268 1917 Fragments arranged by size: 2196 1917 1384 680 354 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6602 AflIII A'CryG_T Cuts at: 1203 5698 6675 1203 Size: 4495 977 1327 Fragments arranged by size: 4495 1327 977 AgeI A'CCGG_T Cuts at: 5630 5630 Size: 6799 AhdI GACnn_n'nnGTC Cuts at: 5528 5528 Size: 6799 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6799 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6799 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3935 3967 3935 Size: 32 6767 AlwNI CAG_nnn'CTG Cuts at: 3371 3543 4542 6114 3371 Size: 172 999 1572 4056 Fragments arranged by size: 4056 1572 999 172 ApaLI G'TGCA_C Cuts at: 4856 6012 4856 Size: 1156 5643 ApoI r'AATT_y Cuts at: 1493 2903 3494 4108 4334 4486 6595 1493 Size: 1410 591 614 226 152 2109 1697 Fragments arranged by size: 2109 1697 1410 614 591 226 152 AseI AT'TA_AT Cuts at: 160 1032 5353 160 Size: 872 4321 1606 Fragments arranged by size: 4321 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6799 AvaI C'yCGr_G Cuts at: 1870 1943 2524 3907 4124 1870 Size: 73 581 1383 217 4545 Fragments arranged by size: 4545 1383 581 217 73 BamHI G'GATC_C Cuts at: 2329 4129 4496 2329 Size: 1800 367 4632 Fragments arranged by size: 4632 1800 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3274 3758 4120 5575 618 Size: 325 217 627 1487 484 362 1455 1842 Fragments arranged by size: 1842 1487 1455 627 484 362 325 217 BanII G_rGCy'C Cuts at: 1391 2026 3563 3808 4118 1391 Size: 635 1537 245 310 4072 Fragments arranged by size: 4072 1537 635 310 245 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3192 928 Size: 971 1293 4535 Fragments arranged by size: 4535 1293 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2216 2250 4990 5024 1903 Size: 34 279 34 2740 34 3678 Fragments arranged by size: 3678 2740 279 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3313 3593 4691 5901 3313 Size: 280 1098 1210 4211 Fragments arranged by size: 4211 1210 1098 280 BclI T'GATC_A Cuts at: 1548 1548 Size: 6799 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2219 5410 136 Size: 107 122 71 631 605 547 3191 1525 Fragments arranged by size: 3191 1525 631 605 547 122 107 71 BglII A'GATC_T Cuts at: 6794 6794 Size: 6799 BlpI GC'TnA_GC Cuts at: 3101 4216 3101 Size: 1115 5684 Bme1580I G_kGCm'C Cuts at: 1790 2539 3517 4860 6016 1790 Size: 749 978 1343 1156 2573 Fragments arranged by size: 2573 1343 1156 978 749 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6799 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2160 3696 4530 Size: 416 53 83 399 24 743 1536 834 Cuts at: 4530 5488 442 Size: 958 1753 Fragments arranged by size: 1753 1536 958 834 743 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6799 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3520 5459 1239 Size: 2281 1939 2579 Fragments arranged by size: 2579 2281 1939 Bpu10I CC'TnA_GC Cuts at: 2481 3562 2481 Size: 1081 5718 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2519 3104 4924 5789 6087 2519 Size: 585 1820 865 298 3231 Fragments arranged by size: 3231 1820 865 585 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3170 5462 882 Size: 617 1671 2292 2219 Fragments arranged by size: 2292 2219 1671 617 BsaAI yAC'GTr Cuts at: 493 1204 2489 4590 493 Size: 711 1285 2101 2702 Fragments arranged by size: 2702 2101 1285 711 BsaBI GATnn'nnATC Cuts at: 6793 6793 Size: 6799 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4606 4988 5623 275 Size: 53 83 186 936 3073 382 635 1451 Fragments arranged by size: 3073 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3030 3927 5232 5630 5904 6051 6462 3030 Size: 897 1305 398 274 147 411 3367 Fragments arranged by size: 3367 1305 897 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3231 3261 1178 Size: 30 165 30 1828 30 4716 Fragments arranged by size: 4716 1828 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2913 3791 1186 Size: 1727 878 4194 Fragments arranged by size: 4194 1727 878 BseYI C'CCAG_C Cuts at: 2438 2565 2657 6002 6498 2438 Size: 127 92 3345 496 2739 Fragments arranged by size: 3345 2739 496 127 92 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2109 4066 1163 Size: 573 373 1957 3896 Fragments arranged by size: 3896 1957 573 373 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3932 4249 5010 6038 1540 Size: 275 186 1931 317 761 1028 2301 Fragments arranged by size: 2301 1931 1028 761 317 275 186 BsiHKAI G_wGCw'C Cuts at: 4860 4945 6016 4860 Size: 85 1071 5643 Fragments arranged by size: 5643 1071 85 BsmI GAATG_Cn' Cuts at: 3080 4310 4403 3080 Size: 1230 93 5476 Fragments arranged by size: 5476 1230 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2026 2539 3517 3563 3808 4118 4860 Size: 399 236 513 978 46 245 310 742 Cuts at: 4860 4945 6016 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 978 742 513 399 310 245 236 85 46 BspEI T'CCGG_A Cuts at: 3030 6462 3030 Size: 3432 3367 BspHI T'CATG_A Cuts at: 3204 3985 4688 6418 3204 Size: 781 703 1730 3585 Fragments arranged by size: 3585 1730 781 703 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2243 2421 2946 4154 844 Size: 1399 178 525 1208 3489 Fragments arranged by size: 3489 1399 1208 525 178 BsrBI CCG'CTC Cuts at: 1394 1823 4686 1394 Size: 429 2863 3507 Fragments arranged by size: 3507 2863 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2214 5294 5468 65 Size: 1183 966 3080 174 1396 Fragments arranged by size: 3080 1396 1183 966 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2761 5443 5630 1700 Size: 110 951 2682 187 2869 Fragments arranged by size: 2869 2682 951 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6799 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6799 BssSI C'ACGA_G Cuts at: 3110 4859 5871 3110 Size: 1749 1012 4038 Fragments arranged by size: 4038 1749 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6799 BstBI TT'CG_AA Cuts at: 5603 5603 Size: 6799 BstEII G'GTnAC_C Cuts at: 1184 4040 1184 Size: 2856 3943 BstXI CCAn_nnnn'nTGG Cuts at: 3232 3232 Size: 6799 BstYI r'GATC_y Cuts at: 1972 2329 4129 4496 4881 4898 6350 6436 6448 Size: 357 1800 367 385 17 1452 86 12 Cuts at: 6448 6794 1972 Size: 346 1977 Fragments arranged by size: 1977 1800 1452 385 367 357 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2016 2959 3450 3707 513 Size: 552 951 943 491 257 3605 Fragments arranged by size: 3605 951 943 552 491 257 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3178 487 Size: 683 56 1952 4108 Fragments arranged by size: 4108 1952 683 56 BtsI GCAGTG_nn' Cuts at: 2097 2514 3021 3357 3478 4315 5109 5129 2097 Size: 417 507 336 121 837 794 20 3767 Fragments arranged by size: 3767 837 794 507 417 336 121 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6799 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6764 DraI TTT'AAA Cuts at: 2970 4103 2970 Size: 1133 5666 DraIII CAC_nnn'GTG Cuts at: 2115 3820 2115 Size: 1705 5094 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5806 809 Size: 507 4490 1802 Fragments arranged by size: 4490 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2511 3798 3975 Size: 54 1425 50 275 18 681 1287 177 Cuts at: 3975 4246 5135 6764 8 Size: 271 889 1629 43 Fragments arranged by size: 1629 1425 1287 889 681 275 271 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4246 1537 Size: 275 2434 4090 Fragments arranged by size: 4090 2434 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4729 1353 Size: 3376 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5386 5760 5906 387 Size: 1064 138 3797 374 146 1280 Fragments arranged by size: 3797 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2312 2666 3520 4862 5459 6246 6514 Size: 393 680 354 854 1342 597 787 268 Cuts at: 6514 1239 Size: 1524 Fragments arranged by size: 1524 1342 854 787 680 597 393 354 268 EcoO109I rG'GnC_Cy Cuts at: 2797 2935 3943 2797 Size: 138 1008 5653 Fragments arranged by size: 5653 1008 138 EcoRI G'AATT_C Cuts at: 2903 4108 4486 2903 Size: 1205 378 5216 Fragments arranged by size: 5216 1205 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2860 2860 Size: 6799 FspI TGC'GCA Cuts at: 5305 5305 Size: 6799 HaeII r_GCGC'y Cuts at: 1678 3621 5946 1678 Size: 1943 2325 2531 Fragments arranged by size: 2531 2325 1943 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3152 3184 3231 3263 3850 Size: 32 135 32 1580 32 47 32 587 Cuts at: 3850 3882 5440 5472 5514 5546 1373 Size: 32 1558 32 42 32 2626 Fragments arranged by size: 2626 1580 1558 587 135 47 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2576 3142 3869 4143 4389 669 Size: 988 281 638 566 727 274 246 3079 Fragments arranged by size: 3079 988 727 638 566 281 274 246 HindIII A'AGCT_T Cuts at: 748 3843 748 Size: 3095 3704 HpaI GTT'AAC Cuts at: 3142 4389 3142 Size: 1247 5552 KpnI G_GTAC'C Cuts at: 3762 4124 3762 Size: 362 6437 MfeI C'AATT_G Cuts at: 4398 4398 Size: 6799 MluI A'CGCG_T Cuts at: 6675 6675 Size: 6799 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2076 2932 3912 5913 6097 1305 Size: 771 856 980 2001 184 2007 Fragments arranged by size: 2007 2001 980 856 771 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2513 3800 3977 10 Size: 54 1425 343 681 1287 177 2832 Fragments arranged by size: 2832 1425 1287 681 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3449 4757 5116 5275 518 Size: 648 2283 1308 359 159 2042 Fragments arranged by size: 2283 2042 1308 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2438 2532 2569 3434 4215 4892 6040 6285 Size: 832 94 37 865 781 677 1148 245 Cuts at: 6285 1606 Size: 2120 Fragments arranged by size: 2120 1148 865 832 781 677 245 94 37 NcoI C'CATG_G Cuts at: 513 1065 3450 3707 513 Size: 552 2385 257 3605 Fragments arranged by size: 3605 2385 552 257 NdeI CA'TA_TG Cuts at: 387 2136 4000 387 Size: 1749 1864 3186 Fragments arranged by size: 3186 1864 1749 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6799 NotI GC'GGCC_GC Cuts at: 4246 4246 Size: 6799 NspI r_CATG'y Cuts at: 1132 1229 3054 4157 5702 1132 Size: 97 1825 1103 1545 2229 Fragments arranged by size: 2229 1825 1545 1103 97 PasI CC'CwG_GG Cuts at: 1348 3518 1348 Size: 2170 4629 PciI A'CATG_T Cuts at: 5698 5698 Size: 6799 PflMI CCAn_nnn'nTGG Cuts at: 1108 2159 2591 1108 Size: 1051 432 5316 Fragments arranged by size: 5316 1051 432 PfoI T'CCnGG_A Cuts at: 4071 4071 Size: 6799 PmeI GTTT'AAAC Cuts at: 4103 4103 Size: 6799 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4868 4900 6381 6413 4868 Size: 32 1481 32 5254 Fragments arranged by size: 5254 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2797 2935 3943 2797 Size: 138 1008 5653 Fragments arranged by size: 5653 1008 138 PshAI GACnn'nnGTC Cuts at: 1613 3537 1613 Size: 1924 4875 PsiI TTA'TAA Cuts at: 4369 4369 Size: 6799 PstI C_TGCA'G Cuts at: 830 3485 4151 830 Size: 2655 666 3478 Fragments arranged by size: 3478 2655 666 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6799 PvuII CAG'CTG Cuts at: 2532 2569 2532 Size: 37 6762 SalI G'TCGA_C Cuts at: 1936 4141 1936 Size: 2205 4594 SbfI CC_TGCA'GG Cuts at: 4151 4151 Size: 6799 ScaI AGT'ACT Cuts at: 5047 6759 5047 Size: 1712 5087 SfcI C'TryA_G Cuts at: 826 1045 3345 3481 4147 5282 5963 6154 826 Size: 219 2300 136 666 1135 681 191 1471 Fragments arranged by size: 2300 1471 1135 681 666 219 191 136 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6799 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6799 SmaI CCC'GGG Cuts at: 1872 4126 1872 Size: 2254 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2498 3119 4903 5804 6066 820 Size: 197 926 555 621 1784 901 262 1553 Fragments arranged by size: 1784 1553 926 901 621 555 262 197 SnaBI TAC'GTA Cuts at: 493 4590 493 Size: 4097 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6799 SphI G_CATG'C Cuts at: 1132 4157 1132 Size: 3025 3774 SspI AAT'ATT Cuts at: 5 52 4723 5 Size: 47 4671 2081 Fragments arranged by size: 4671 2081 47 StuI AGG'CCT Cuts at: 1517 3567 1517 Size: 2050 4749 StyI C'CwwG_G Cuts at: 513 1065 3450 3707 3939 4238 513 Size: 552 2385 257 232 299 3074 Fragments arranged by size: 3074 2385 552 299 257 232 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2430 4826 4843 4996 5181 5626 325 Size: 1255 850 2396 17 153 185 445 1498 Fragments arranged by size: 2396 1498 1255 850 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5045 6757 96 Size: 274 80 33 51 1443 3068 1712 138 Fragments arranged by size: 3068 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6759 6791 6759 Size: 32 6767 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6799 XbaI T'CTAG_A Cuts at: 4135 4135 Size: 6799 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2879 3001 3419 3769 2879 Size: 122 418 350 5909 Fragments arranged by size: 5909 418 350 122 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6799 XmaI C'CCGG_G Cuts at: 1870 4124 1870 Size: 2254 4545 XmnI GAAnn'nnTTC Cuts at: 4928 4928 Size: 6799 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4607 276 Size: 53 83 186 936 3073 2468 Fragments arranged by size: 3073 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI