(Linear) (Six-base) MAPSORT of: KIEE2058.seq Check: 6416 from: 1 to: 2094 KIEE2058.orf With 182 enzymes: SgfI * July 15, 2010 18:54 .. AccI GT'mk_AC Cuts at: 0 1244 2088 2094 Size: 1244 844 6 Fragments arranged by size: 1244 844 6 Acc65I G'GTAC_C Cuts at: 0 1753 2094 Size: 1753 341 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 307 661 2094 Size: 307 354 1433 Fragments arranged by size: 1433 354 307 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1930 1962 2094 Size: 1930 32 132 Fragments arranged by size: 1930 132 32 AlwNI CAG_nnn'CTG Cuts at: 0 1366 1538 2094 Size: 1366 172 556 Fragments arranged by size: 1366 556 172 ApoI r'AATT_y Cuts at: 0 898 1489 2094 Size: 898 591 605 Fragments arranged by size: 898 605 591 AvaI C'yCGr_G Cuts at: 0 519 1902 2094 Size: 519 1383 192 Fragments arranged by size: 1383 519 192 BamHI G'GATC_C Cuts at: 0 324 2094 Size: 324 1770 BanI G'GyrC_C Cuts at: 0 1269 1753 2094 Size: 1269 484 341 Fragments arranged by size: 1269 484 341 BanII G_rGCy'C Cuts at: 0 21 1558 1803 2094 Size: 21 1537 245 291 Fragments arranged by size: 1537 291 245 21 BbsI GAAGACnn'nnnn_ Cuts at: 0 1187 2094 Size: 1187 907 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 211 245 2094 Size: 211 34 1849 Fragments arranged by size: 1849 211 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1308 1588 2094 Size: 1308 280 506 Fragments arranged by size: 1308 506 280 BglI GCCn_nnn'nGGC Cuts at: 0 214 2094 Size: 214 1880 BlpI GC'TnA_GC Cuts at: 0 1096 2094 Size: 1096 998 Bme1580I G_kGCm'C Cuts at: 0 534 1512 2094 Size: 534 978 582 Fragments arranged by size: 978 582 534 BmrI ACTGGGnnnn_n' Cuts at: 0 155 1691 2094 Size: 155 1536 403 Fragments arranged by size: 1536 403 155 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1515 2094 Size: 1515 579 Bpu10I CC'TnA_GC Cuts at: 0 476 1557 2094 Size: 476 1081 537 Fragments arranged by size: 1081 537 476 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 514 1099 2094 Size: 514 585 995 Fragments arranged by size: 995 585 514 BsaI GGTCTCn'nnnn_ Cuts at: 0 1165 2094 Size: 1165 929 BsaAI yAC'GTr Cuts at: 0 484 2094 Size: 484 1610 BsaWI w'CCGG_w Cuts at: 0 1025 1922 2094 Size: 1025 897 172 Fragments arranged by size: 1025 897 172 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1226 1256 2094 Size: 1226 30 838 Fragments arranged by size: 1226 838 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 908 1786 2094 Size: 908 878 308 Fragments arranged by size: 908 878 308 BseYI C'CCAG_C Cuts at: 0 433 560 652 2094 Size: 433 127 92 1442 Fragments arranged by size: 1442 433 127 92 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 104 2061 2094 Size: 104 1957 33 Fragments arranged by size: 1957 104 33 BsiEI CG_ry'CG Cuts at: 0 1927 2094 Size: 1927 167 BsmI GAATG_Cn' Cuts at: 0 1075 2094 Size: 1075 1019 Bsp1286I G_dGCh'C Cuts at: 0 21 534 1512 1558 1803 2094 Size: 21 513 978 46 245 291 Fragments arranged by size: 978 513 291 245 46 21 BspEI T'CCGG_A Cuts at: 0 1025 2094 Size: 1025 1069 BspHI T'CATG_A Cuts at: 0 1199 1980 2094 Size: 1199 781 114 Fragments arranged by size: 1199 781 114 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 238 416 941 2094 Size: 238 178 525 1153 Fragments arranged by size: 1153 525 238 178 BsrDI GCAATG_nn' Cuts at: 0 209 2094 Size: 209 1885 BsrFI r'CCGG_y Cuts at: 0 756 2094 Size: 756 1338 BssSI C'ACGA_G Cuts at: 0 1105 2094 Size: 1105 989 BstEII G'GTnAC_C Cuts at: 0 2035 2094 Size: 2035 59 BstXI CCAn_nnnn'nTGG Cuts at: 0 1227 2094 Size: 1227 867 BstYI r'GATC_y Cuts at: 0 324 2094 Size: 324 1770 BtgI C'CryG_G Cuts at: 0 11 954 1445 1702 2094 Size: 11 943 491 257 392 Fragments arranged by size: 943 491 392 257 11 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1173 2094 Size: 1173 921 BtsI GCAGTG_nn' Cuts at: 0 92 509 1016 1352 1473 2094 Size: 92 417 507 336 121 621 Fragments arranged by size: 621 507 417 336 121 92 DraI TTT'AAA Cuts at: 0 965 2094 Size: 965 1129 DraIII CAC_nnn'GTG Cuts at: 0 110 1815 2094 Size: 110 1705 279 Fragments arranged by size: 1705 279 110 EaeI y'GGCC_r Cuts at: 0 506 1793 1970 2094 Size: 506 1287 177 124 Fragments arranged by size: 1287 506 177 124 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 307 661 1515 2094 Size: 307 354 854 579 Fragments arranged by size: 854 579 354 307 EcoO109I rG'GnC_Cy Cuts at: 0 792 930 1938 2094 Size: 792 138 1008 156 Fragments arranged by size: 1008 792 156 138 EcoRI G'AATT_C Cuts at: 0 898 2094 Size: 898 1196 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 855 2094 Size: 855 1239 HaeII r_GCGC'y Cuts at: 0 1616 2094 Size: 1616 478 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1147 1179 1226 1258 1845 1877 2094 Size: 1147 32 47 32 587 32 217 Fragments arranged by size: 1147 587 217 47 32 32 32 HincII GTy'rAC Cuts at: 0 571 1137 1864 2094 Size: 571 566 727 230 Fragments arranged by size: 727 571 566 230 HindIII A'AGCT_T Cuts at: 0 1838 2094 Size: 1838 256 HpaI GTT'AAC Cuts at: 0 1137 2094 Size: 1137 957 KpnI G_GTAC'C Cuts at: 0 1757 2094 Size: 1757 337 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 71 927 1907 2094 Size: 71 856 980 187 Fragments arranged by size: 980 856 187 71 MscI TGG'CCA Cuts at: 0 508 1795 1972 2094 Size: 508 1287 177 122 Fragments arranged by size: 1287 508 177 122 MslI CAynn'nnrTG Cuts at: 0 1444 2094 Size: 1444 650 MspA1I CmG'CkG Cuts at: 0 433 527 564 1429 2094 Size: 433 94 37 865 665 Fragments arranged by size: 865 665 433 94 37 NcoI C'CATG_G Cuts at: 0 1445 1702 2094 Size: 1445 257 392 Fragments arranged by size: 1445 392 257 NdeI CA'TA_TG Cuts at: 0 131 1995 2094 Size: 131 1864 99 Fragments arranged by size: 1864 131 99 NspI r_CATG'y Cuts at: 0 1049 2094 Size: 1049 1045 PasI CC'CwG_GG Cuts at: 0 1513 2094 Size: 1513 581 PflMI CCAn_nnn'nTGG Cuts at: 0 154 586 2094 Size: 154 432 1508 Fragments arranged by size: 1508 432 154 PfoI T'CCnGG_A Cuts at: 0 2066 2094 Size: 2066 28 PpuMI rG'GwC_Cy Cuts at: 0 792 930 1938 2094 Size: 792 138 1008 156 Fragments arranged by size: 1008 792 156 138 PshAI GACnn'nnGTC Cuts at: 0 1532 2094 Size: 1532 562 PstI C_TGCA'G Cuts at: 0 1480 2094 Size: 1480 614 PvuII CAG'CTG Cuts at: 0 527 564 2094 Size: 527 37 1530 Fragments arranged by size: 1530 527 37 SfcI C'TryA_G Cuts at: 0 1340 1476 2094 Size: 1340 136 618 Fragments arranged by size: 1340 618 136 SmlI C'TyrA_G Cuts at: 0 493 1114 2094 Size: 493 621 980 Fragments arranged by size: 980 621 493 StuI AGG'CCT Cuts at: 0 1562 2094 Size: 1562 532 StyI C'CwwG_G Cuts at: 0 1445 1702 1934 2094 Size: 1445 257 232 160 Fragments arranged by size: 1445 257 232 160 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 425 2094 Size: 425 1669 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 874 996 1414 1764 2094 Size: 874 122 418 350 330 Fragments arranged by size: 874 418 350 330 122 Enzymes that do cut: AccI Acc65I AcuI AloI AlwNI ApoI AvaI BamHI BanI BanII BbsI BcgI BciVI BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BssSI BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII EaeI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII HpaI KpnI MmeI MscI MslI MspA1I NcoI NdeI NspI PasI PflMI PfoI PpuMI PshAI PstI PvuII SfcI SmlI StuI StyI TaqII XcmI Enzymes that do not cut: AarI AatII AclI AfeI AflII AflIII AgeI AhdI AleI AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BclI BfrBI BglII BmgBI BmtI BplI BsaBI BsaHI BsiHKAI BsiWI BsmBI BsrBI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DrdI EagI EarI EciI EcoICRI EcoNI EcoRV FseI FspI FspAI KasI MfeI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI