################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvaI AvaII AvrII BalI BamHI BanII BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaI BsaJI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HpaI KpnI KspI MamI MluI MluNI MscI MvnI NaeI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TaqI Tth111I TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccI 559 GTCTAC GT^[AC][GT]AC AccIII 56 TCCGGA T^CCGGA AcyI 31 GGCGCC G[GA]^CG[CT]C AflIII 771 ACGTGT A^C[GA][CT]GT AlwNI 1273 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 646 ATTAAT AT^TAAT AsnI 646 ATTAAT AT^TAAT Asp700I 700 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 23 TGCGCA TGC^GCA BanI 31 GGCGCC G^G[CT][GA]CC BbeI 31 GGCGCC GGCGC^C BbsI 801 GAAGAC GAAGAC(2/6) BfaI 1081 CTAG C^TAG BpmI 1169 CTGGAG CTGGAG(16/14) BpuAI 801 GAAGAC GAAGAC(2/6) BsaHI 31 GGCGCC G[GA]^CG[CT]C BsaWI 56 TCCGGA [AT]^CCGG[AT] BseAI 56 TCCGGA T^CCGGA BsmFI 1149 GGGAC GGGAC(10/14) BspEI 56 TCCGGA T^CCGGA BspMI 448 GCAGGT ACCTGC(4/8) BssKI 1175 CCAGG ^CC[ACGT]GG BstNI 1175 CCAGG CC^[AT]GG BstXI 395 CCATCATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 157 GGATCT [GA]^GATC[CT] EarI 903 GAAGAG CTCTTC(1/4) EcoRII 1175 CCAGG ^CC[AT]GG FauI 35 CCCGC CCCGC(4/6) FspI 23 TGCGCA TGC^GCA HaeII 31 GGCGCC [GA]GCGC^[CT] HaeIII 405 GGCC GG^CC HapII 57 CCGG C^CGG Hin1I 31 GGCGCC G[GA]^CG[CT]C HincII 731 GTCAAC GT[CT]^[GA]AC HindII 731 GTCAAC GT[CT]^[GA]AC HpaII 57 CCGG C^CGG KasI 31 GGCGCC G^GCGCC Ksp632I 903 GAAGAG CTCTTC(1/4) MaeI 1081 CTAG C^TAG MaeII 771 ACGT A^CGT MflI 157 GGATCT [GA]^GATC[CT] MroI 56 TCCGGA T^CCGGA MspI 57 CCGG C^CGG MvaI 1175 CCAGG CC^[AT]GG NarI 31 GGCGCC GG^CGCC NspI 464 GCATGT [GA]CATG^[CT] PflMI 505 CCATATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1194 GACTC GAGTC(4/5) PshBI 646 ATTAAT AT^TAAT ScrFI 1175 CCAGG CC^[ACGT]GG SfcI 956 CTGTAG C^T[GA][CT]AG SfoI 31 GGCGCC GGC^GCC SspI 613 AATATT AAT^ATT TfiI 240 GATTC G^A[AT]TC Tsp45I 712 GTCAC ^GT[GC]AC Van91I 505 CCATATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 813 CCAGAAAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 157 GGATCT [GA]^GATC[CT] XmnI 700 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AcsI 569 AAATTC [GA]^AATT[CT] 688 AAATTT ApoI 569 AAATTC [GA]^AATT[CT] 688 AAATTT BsgI 67 GTGCAG GTGCAG(16/14) 329 GTGCAG BspHI 398 TCATGA T^CATGA 884 TCATGA CfoI 24 GCGC GCG^C 32 GCGC DdeI 194 CTAAG C^T[ACGT]AG 590 CTGAG HgaI 634 GACGC GACGC(5/10) 949 GACGC HhaI 24 GCGC GCG^C 32 GCGC HinP1I 24 GCGC G^CGC 32 GCGC HindIII 196 AAGCTT A^AGCTT 520 AAGCTT HinfI 240 GATTC G^A[ACGT]TC 1194 GACTC HphI 292 GGTGA GGTGA(8/7) 747 GGTGA MfeI 977 CAATTG C^AATTG 1157 CAATTG MunI 977 CAATTG C^AATTG 1157 CAATTG RcaI 398 TCATGA T^CATGA 884 TCATGA SfaNI 895 GATGC GCATC(5/9) 997 GCATC TspRI 854 CACTG CA[GC]TG[ACGT][ACGT]^ 923 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AciI AfaI AluI AlwI BbvI BseRI BsiYI BslI BsrDI BsrI BstF5I Cac8I DpnII Fnu4HI FokI ItaI MaeIII MboI MboII MnlI MseI MslI MspA1I MwoI NdeI NdeII NlaIII NlaIV PvuII RsaI Sau3AI Tru9I TseI Tsp509I