(Circular) (Six-base) MAPSORT of: pFN21AE1240.seq Check: 479 from: 1 to: 5986 pFN21AE1240 5986 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2444 2444 Size: 5986 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3796 278 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2565 3329 1937 Size: 628 764 4594 Fragments arranged by size: 4594 764 628 Acc65I G'GTAC_C Cuts at: 3307 3307 Size: 5986 AclI AA'CG_TT Cuts at: 1563 4113 4486 1563 Size: 2550 373 3063 Fragments arranged by size: 3063 2550 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2370 2825 4049 5433 5701 1632 Size: 738 455 1224 1384 268 1917 Fragments arranged by size: 1917 1384 1224 738 455 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5789 AflIII A'CryG_T Cuts at: 1203 2776 4885 5862 1203 Size: 1573 2109 977 1327 Fragments arranged by size: 2109 1573 1327 977 AgeI A'CCGG_T Cuts at: 4817 4817 Size: 5986 AhdI GACnn_n'nnGTC Cuts at: 4715 4715 Size: 5986 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5986 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2072 1184 Size: 888 5098 AlwNI CAG_nnn'CTG Cuts at: 3283 3729 5301 3283 Size: 446 1572 3968 Fragments arranged by size: 3968 1572 446 ApaLI G'TGCA_C Cuts at: 4043 5199 4043 Size: 1156 4830 ApoI r'AATT_y Cuts at: 1493 2574 2693 3295 3521 3673 5782 1493 Size: 1081 119 602 226 152 2109 1697 Fragments arranged by size: 2109 1697 1081 602 226 152 119 AseI AT'TA_AT Cuts at: 160 1032 2652 4540 160 Size: 872 1620 1888 1606 Fragments arranged by size: 1888 1620 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 AvaI C'yCGr_G Cuts at: 1870 1943 3311 1870 Size: 73 1368 4545 Fragments arranged by size: 4545 1368 73 BamHI G'GATC_C Cuts at: 3316 3683 3316 Size: 367 5619 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2036 3307 4762 618 Size: 325 217 627 249 1271 1455 1842 Fragments arranged by size: 1842 1455 1271 627 325 249 217 BanII G_rGCy'C Cuts at: 1391 3305 1391 Size: 1914 4072 BbeI G_GCGC'C Cuts at: 2040 2040 Size: 5986 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2813 928 Size: 971 914 4101 Fragments arranged by size: 4101 971 914 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4177 4211 1903 Size: 34 2240 34 3678 Fragments arranged by size: 3678 2240 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3878 5088 3878 Size: 1210 4776 BclI T'GATC_A Cuts at: 1548 1548 Size: 5986 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4597 136 Size: 107 122 71 631 605 2925 1525 Fragments arranged by size: 2925 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5981 5981 Size: 5986 BlpI GC'TnA_GC Cuts at: 3403 3403 Size: 5986 Bme1580I G_kGCm'C Cuts at: 1790 4047 5203 1790 Size: 2257 1156 2573 Fragments arranged by size: 2573 2257 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5986 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3717 4675 442 Size: 416 53 83 399 24 2300 958 1753 Fragments arranged by size: 2300 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5986 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3195 4646 1239 Size: 1956 1451 2579 Fragments arranged by size: 2579 1956 1451 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4111 4976 5274 4111 Size: 865 298 4823 Fragments arranged by size: 4823 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4649 882 Size: 617 3150 2219 Fragments arranged by size: 3150 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3777 493 Size: 711 2573 2702 Fragments arranged by size: 2702 2573 711 BsaBI GATnn'nnATC Cuts at: 5980 5980 Size: 5986 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2037 3793 4175 4810 Size: 53 83 186 936 504 1756 382 635 Cuts at: 4810 275 Size: 1451 Fragments arranged by size: 1756 1451 936 635 504 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2061 4419 4817 5091 5238 5649 2061 Size: 2358 398 274 147 411 2398 Fragments arranged by size: 2398 2358 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2456 2486 3129 3159 1178 Size: 30 165 30 1053 30 643 30 4005 Fragments arranged by size: 4005 1053 643 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3153 3264 3267 1186 Size: 1967 111 3 3905 Fragments arranged by size: 3905 1967 111 3 BseYI C'CCAG_C Cuts at: 2687 5189 5685 2687 Size: 2502 496 2988 Fragments arranged by size: 2988 2502 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2093 2355 1163 Size: 573 357 262 4794 Fragments arranged by size: 4794 573 357 262 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3436 4197 5225 1540 Size: 275 186 1435 761 1028 2301 Fragments arranged by size: 2301 1435 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4047 4132 5203 4047 Size: 85 1071 4830 Fragments arranged by size: 4830 1071 85 BsmI GAATG_Cn' Cuts at: 3497 3590 3497 Size: 93 5893 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3305 4047 4132 5203 1391 Size: 399 1515 742 85 1071 2174 Fragments arranged by size: 2174 1515 1071 742 399 85 BspEI T'CCGG_A Cuts at: 2061 5649 2061 Size: 3588 2398 BspHI T'CATG_A Cuts at: 2403 2889 3875 5605 2403 Size: 486 986 1730 2784 Fragments arranged by size: 2784 1730 986 486 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2444 3341 844 Size: 1600 897 3489 Fragments arranged by size: 3489 1600 897 BsrBI CCG'CTC Cuts at: 1394 1823 3873 1394 Size: 429 2050 3507 Fragments arranged by size: 3507 2050 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2211 3054 3250 4481 4655 65 Size: 1183 963 843 196 1231 174 1396 Fragments arranged by size: 1396 1231 1183 963 843 196 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4630 4817 1700 Size: 110 2820 187 2869 Fragments arranged by size: 2869 2820 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5986 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5986 BssSI C'ACGA_G Cuts at: 4046 5058 4046 Size: 1012 4974 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5986 BstBI TT'CG_AA Cuts at: 4790 4790 Size: 5986 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5986 BstXI CCAn_nnnn'nTGG Cuts at: 2407 2407 Size: 5986 BstYI r'GATC_y Cuts at: 1972 2162 3316 3683 4068 4085 5537 5623 5635 Size: 190 1154 367 385 17 1452 86 12 Cuts at: 5635 5981 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1154 385 367 346 190 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4495 Fragments arranged by size: 4495 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5247 Fragments arranged by size: 5247 683 56 BtsI GCAGTG_nn' Cuts at: 3502 4296 4316 3502 Size: 794 20 5172 Fragments arranged by size: 5172 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5986 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2185 2220 529 Size: 35 1621 35 4295 Fragments arranged by size: 4295 1621 35 35 DraI TTT'AAA Cuts at: 3290 3290 Size: 5986 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4993 809 Size: 507 3677 1802 Fragments arranged by size: 3677 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3433 4322 5951 Size: 54 1425 50 275 18 1603 889 1629 Cuts at: 5951 8 Size: 43 Fragments arranged by size: 1629 1603 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3433 1537 Size: 275 1621 4090 Fragments arranged by size: 4090 1621 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2903 3916 1353 Size: 1550 1013 3423 Fragments arranged by size: 3423 1550 1013 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4573 4947 5093 387 Size: 1064 138 2984 374 146 1280 Fragments arranged by size: 2984 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2370 2825 3195 4049 4646 5433 5701 Size: 393 738 455 370 854 597 787 268 Cuts at: 5701 1239 Size: 1524 Fragments arranged by size: 1524 854 787 738 597 455 393 370 268 EcoRI G'AATT_C Cuts at: 3295 3673 3295 Size: 378 5608 FspI TGC'GCA Cuts at: 2030 4492 2030 Size: 2462 3524 HaeII r_GCGC'y Cuts at: 1678 2040 5133 1678 Size: 362 3093 2531 Fragments arranged by size: 3093 2531 362 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2398 2430 2456 2488 3175 Size: 32 135 32 826 32 26 32 687 Cuts at: 3175 3207 4627 4659 4701 4733 1373 Size: 32 1420 32 42 32 2626 Fragments arranged by size: 2626 1420 826 687 135 42 32 32 32 32 32 32 32 26 HincII GTy'rAC Cuts at: 669 1657 1938 2738 3330 3576 669 Size: 988 281 800 592 246 3079 Fragments arranged by size: 3079 988 800 592 281 246 HindIII A'AGCT_T Cuts at: 748 2201 2525 748 Size: 1453 324 4209 Fragments arranged by size: 4209 1453 324 HpaI GTT'AAC Cuts at: 3576 3576 Size: 5986 KasI G'GCGC_C Cuts at: 2036 2036 Size: 5986 KpnI G_GTAC'C Cuts at: 3311 3311 Size: 5986 MfeI C'AATT_G Cuts at: 2982 3162 3585 2982 Size: 180 423 5383 Fragments arranged by size: 5383 423 180 MluI A'CGCG_T Cuts at: 5862 5862 Size: 5986 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3084 5100 5284 1305 Size: 1779 2016 184 2007 Fragments arranged by size: 2016 2007 1779 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4164 Fragments arranged by size: 4164 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2405 2439 3098 3944 4303 4462 Size: 648 843 396 34 659 846 359 159 Cuts at: 4462 518 Size: 2042 Fragments arranged by size: 2042 846 843 659 648 396 359 159 34 MspA1I CmG'CkG Cuts at: 1606 2034 2589 2843 2876 3402 4079 5227 5472 Size: 428 555 254 33 526 677 1148 245 Cuts at: 5472 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 555 526 428 254 245 33 NarI GG'CG_CC Cuts at: 2037 2037 Size: 5986 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4495 Fragments arranged by size: 4495 939 552 NdeI CA'TA_TG Cuts at: 387 2485 2512 2580 387 Size: 2098 27 68 3793 Fragments arranged by size: 3793 2098 68 27 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5986 NotI GC'GGCC_GC Cuts at: 3433 3433 Size: 5986 NspI r_CATG'y Cuts at: 1132 1229 2473 3344 4889 1132 Size: 97 1244 871 1545 2229 Fragments arranged by size: 2229 1545 1244 871 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5986 PciI A'CATG_T Cuts at: 4885 4885 Size: 5986 PflMI CCAn_nnn'nTGG Cuts at: 1108 2516 1108 Size: 1408 4578 PmeI GTTT'AAAC Cuts at: 3290 3290 Size: 5986 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4055 4087 5568 5600 4055 Size: 32 1481 32 4441 Fragments arranged by size: 4441 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5986 PsiI TTA'TAA Cuts at: 3556 3556 Size: 5986 PstI C_TGCA'G Cuts at: 830 3338 830 Size: 2508 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5986 PvuII CAG'CTG Cuts at: 2589 2843 2876 2589 Size: 254 33 5699 Fragments arranged by size: 5699 254 33 SalI G'TCGA_C Cuts at: 1936 3328 1936 Size: 1392 4594 SbfI CC_TGCA'GG Cuts at: 3338 3338 Size: 5986 ScaI AGT'ACT Cuts at: 4234 5946 4234 Size: 1712 4274 SfcI C'TryA_G Cuts at: 826 1045 2961 3334 4469 5150 5341 826 Size: 219 1916 373 1135 681 191 1471 Fragments arranged by size: 1916 1471 1135 681 373 219 191 SfoI GGC'GCC Cuts at: 2038 2038 Size: 5986 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5986 SmaI CCC'GGG Cuts at: 1872 3313 1872 Size: 1441 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4090 4991 5253 820 Size: 197 926 2147 901 262 1553 Fragments arranged by size: 2147 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3777 493 Size: 3284 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5986 SphI G_CATG'C Cuts at: 1132 3344 1132 Size: 2212 3774 SspI AAT'ATT Cuts at: 5 52 2620 3910 5 Size: 47 2568 1290 2081 Fragments arranged by size: 2568 2081 1290 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5986 StyI C'CwwG_G Cuts at: 513 1065 2004 3425 513 Size: 552 939 1421 3074 Fragments arranged by size: 3074 1421 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2170 2321 4013 4030 4183 4368 4813 Size: 1255 590 151 1692 17 153 185 445 Cuts at: 4813 325 Size: 1498 Fragments arranged by size: 1692 1498 1255 590 445 185 153 151 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2855 3082 4232 Size: 274 80 33 51 1443 878 227 1150 Cuts at: 4232 5944 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1150 878 274 227 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5946 5978 5946 Size: 32 5954 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5986 XbaI T'CTAG_A Cuts at: 3322 3322 Size: 5986 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2825 2011 Size: 814 5172 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5986 XmaI C'CCGG_G Cuts at: 1870 3311 1870 Size: 1441 4545 XmnI GAAnn'nnTTC Cuts at: 2709 4115 2709 Size: 1406 4580 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3794 276 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI