Restriction map for
pFN21AE1240
(Linear) (Six-base) MAPSORT of: KIEE1240.seq Check: 6096 from: 1 to: 1281
KIEE1240.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 439 1281
Size: 439 842
AccI GT'mk_AC
Cuts at: 0 560 1281
Size: 560 721
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 365 820 1281
Size: 365 455 461
Fragments arranged by size:
461 455 365
AflIII A'CryG_T
Cuts at: 0 771 1281
Size: 771 510
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 67 1281
Size: 67 1214
AlwNI CAG_nnn'CTG
Cuts at: 0 1278 1281
Size: 1278 3
ApoI r'AATT_y
Cuts at: 0 569 688 1281
Size: 569 119 593
Fragments arranged by size:
593 569 119
AseI AT'TA_AT
Cuts at: 0 647 1281
Size: 647 634
BanI G'GyrC_C
Cuts at: 0 31 1281
Size: 31 1250
BbeI G_GCGC'C
Cuts at: 0 35 1281
Size: 35 1246
BbsI GAAGACnn'nnnn_
Cuts at: 0 808 1281
Size: 808 473
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1190 1281
Size: 1190 91
BsaHI Gr'CG_yC
Cuts at: 0 32 1281
Size: 32 1249
BsaWI w'CCGG_w
Cuts at: 0 56 1281
Size: 56 1225
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 451 481 1124 1154 1281
Size: 451 30 643 30 127
Fragments arranged by size:
643 451 127 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1148 1259 1262 1281
Size: 1148 111 3 19
Fragments arranged by size:
1148 111 19 3
BseYI C'CCAG_C
Cuts at: 0 682 1281
Size: 682 599
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 350 1281
Size: 88 262 931
Fragments arranged by size:
931 262 88
BspEI T'CCGG_A
Cuts at: 0 56 1281
Size: 56 1225
BspHI T'CATG_A
Cuts at: 0 398 884 1281
Size: 398 486 397
Fragments arranged by size:
486 398 397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 439 1281
Size: 439 842
BsrDI GCAATG_nn'
Cuts at: 0 206 1049 1245 1281
Size: 206 843 196 36
Fragments arranged by size:
843 206 196 36
BstXI CCAn_nnnn'nTGG
Cuts at: 0 402 1281
Size: 402 879
BstYI r'GATC_y
Cuts at: 0 157 1281
Size: 157 1124
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 180 215 1281
Size: 180 35 1066
Fragments arranged by size:
1066 180 35
EarI CTCTTCn'nnn_
Cuts at: 0 898 1281
Size: 898 383
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 365 820 1190 1281
Size: 365 455 370 91
Fragments arranged by size:
455 370 365 91
FspI TGC'GCA
Cuts at: 0 25 1281
Size: 25 1256
HaeII r_GCGC'y
Cuts at: 0 35 1281
Size: 35 1246
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 393 425 451 483 1170 1202 1281
Size: 393 32 26 32 687 32 79
Fragments arranged by size:
687 393 79 32 32 32 26
HincII GTy'rAC
Cuts at: 0 733 1281
Size: 733 548
HindIII A'AGCT_T
Cuts at: 0 196 520 1281
Size: 196 324 761
Fragments arranged by size:
761 324 196
KasI G'GCGC_C
Cuts at: 0 31 1281
Size: 31 1250
MfeI C'AATT_G
Cuts at: 0 977 1157 1281
Size: 977 180 124
Fragments arranged by size:
977 180 124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1079 1281
Size: 1079 202
MslI CAynn'nnrTG
Cuts at: 0 400 434 1093 1281
Size: 400 34 659 188
Fragments arranged by size:
659 400 188 34
MspA1I CmG'CkG
Cuts at: 0 29 584 838 871 1281
Size: 29 555 254 33 410
Fragments arranged by size:
555 410 254 33 29
NarI GG'CG_CC
Cuts at: 0 32 1281
Size: 32 1249
NdeI CA'TA_TG
Cuts at: 0 480 507 575 1281
Size: 480 27 68 706
Fragments arranged by size:
706 480 68 27
NspI r_CATG'y
Cuts at: 0 468 1281
Size: 468 813
PflMI CCAn_nnn'nTGG
Cuts at: 0 511 1281
Size: 511 770
PvuII CAG'CTG
Cuts at: 0 584 838 871 1281
Size: 584 254 33 410
Fragments arranged by size:
584 410 254 33
SfcI C'TryA_G
Cuts at: 0 956 1281
Size: 956 325
SfoI GGC'GCC
Cuts at: 0 33 1281
Size: 33 1248
SspI AAT'ATT
Cuts at: 0 615 1281
Size: 615 666
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 165 316 1281
Size: 165 151 965
Fragments arranged by size:
965 165 151
TatI w'GTAC_w
Cuts at: 0 850 1077 1281
Size: 850 227 204
Fragments arranged by size:
850 227 204
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 820 1281
Size: 820 461
XmnI GAAnn'nnTTC
Cuts at: 0 704 1281
Size: 704 577
Enzymes that do cut:
AarI AccI AcuI AflIII AlfI AlwNI ApoI AseI
BanI BbeI BbsI BpmI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BspEI BspHI BspMI BsrDI BstXI BstYI
CspCI EarI Eco57MI FspI HaeII Hin4I HincII HindIII
KasI MfeI MmeI MslI MspA1I NarI NdeI NspI
PflMI PvuII SfcI SfoI SspI TaqII TatI XcmI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BanII BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspAI HpaI KpnI
MluI MscI NaeI NcoI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI StuI StyI SwaI TstI Tth111I XbaI
XhoI XmaI ZraI