################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII AccI AccIII AclI AflII AflIII AgeI AocI Aor51HI ApaI AscI AseI AsnI AspI AvaI AvrII BbrPI BcgI BfrI BlnI BlpI Bpu1102I BseAI BsiWI BsmI BsoBI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrGI BssHII Bst1107I BstBI BstZ17I Bsu36I CelII CpoI DraI DraII DraIII DsaI EagI EclXI Eco47III Eco52I Eco81I EcoO109I EcoRI EcoRV EcoT22I FseI HpaI KspI MfeI MluI MroI MslI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### Acc65I 927 GGTACC G^GTACC AcyI 65 GGCGCC G[GA]^CG[CT]C AhdI 854 GACGCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1580 GTGCAC G^TGCAC ApaLI 1580 GTGCAC G^TGCAC Asp700I 1705 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 927 GGTACC G^GTACC AspEI 854 GACGCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 443 TGCGCA TGC^GCA BalI 1010 TGGCCA TGG^CCA BamHI 1345 GGATCC G^GATCC BbeI 65 GGCGCC GGCGC^C BbvCI 1660 GCTGAGG CCTCAGC(-5/-2) BciVI 894 GGATAC GTATCC(6/5) BclI 744 TGATCA T^GATCA BfaI 436 CTAG C^TAG BglI 1628 GCCCTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 138 AGATCT A^GATCT BsaAI 1642 TACGTG [CT]AC^GT[GA] BsaHI 65 GGCGCC G[GA]^CG[CT]C BsaI 757 GAGACC GGTCTC(1/5) BsaWI 1286 ACCGGA [AT]^CCGG[AT] BseRI 97 GAGGAG GAGGAG(10/8) BsiEI 1284 CGACCG CG[GA][CT]^CG BspDI 1193 ATCGAT AT^CGAT BspMI 440 ACCTGC ACCTGC(4/8) BsrFI 74 ACCGGC [GA]^CCGG[CT] BssSI 199 CACGAG CACGAG(-5/-1) BstEII 570 GGTCACC G^GT[ACGT]ACC BstPI 570 GGTCACC G^GT[ACGT]ACC BstXI 575 CCAGATGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 74 ACCGGC [GA]^CCGG[CT] ClaI 1193 ATCGAT AT^CGAT DrdI 715 GACATTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 1010 TGGCCA [CT]^GGCC[GA] Eam1105I 854 GACGCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 811 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 570 GGTCACC G^GT[ACGT]ACC EcoT14I 524 CCTTGG C^C[AT][AT]GG FauI 32 CCCGC CCCGC(4/6) FbaI 744 TGATCA T^GATCA FspI 443 TGCGCA TGC^GCA HgaI 854 GACGC GACGC(5/10) Hin1I 65 GGCGCC G[GA]^CG[CT]C HincII 973 GTCAAC GT[CT]^[GA]AC HindII 973 GTCAAC GT[CT]^[GA]AC KasI 65 GGCGCC G^GCGCC KpnI 927 GGTACC GGTAC^C MaeI 436 CTAG C^TAG MluNI 1010 TGGCCA TGG^CCA MscI 1010 TGGCCA TGG^CCA NarI 65 GGCGCC GG^CGCC PflMI 561 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 1504 GAGCTC GAGCT^C SfoI 65 GGCGCC GGC^GCC StuI 819 AGGCCT AGG^CCT StyI 524 CCTTGG C^C[AT][AT]GG Van91I 561 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 1705 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccII 16 CGCG CG^CG 1262 CGCG AcsI 1117 GAATTT [GA]^AATT[CT] 1409 AAATTC ApoI 1117 GAATTT [GA]^AATT[CT] 1409 AAATTC BanI 65 GGCGCC G^G[CT][GA]CC 927 GGTACC BanII 1504 GAGCTC G[GA]GC[CT]^C 1665 GGGCTC BbsI 949 GTCTTC GAAGAC(2/6) 1621 GTCTTC BpuAI 949 GTCTTC GAAGAC(2/6) 1621 GTCTTC BsaBI 895 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1186 GATGATTATC BsgI 1368 CTGCAC GTGCAG(16/14) 982 GTGCAG BsmBI 424 GAGACG CGTCTC(1/5) 852 GAGACG BsrDI 1355 GCAATG GCAATG(2/0) 1377 GCAATG BstUI 16 CGCG CG^CG 1262 CGCG HaeII 41 AGCGCC [GA]GCGC^[CT] 65 GGCGCC HindIII 451 AAGCTT A^AGCTT 1039 AAGCTT HphI 327 GGTGA GGTGA(8/7) 572 TCACC MamI 895 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1186 GATGATTATC MvnI 16 CGCG CG^CG 1262 CGCG TfiI 829 GATTC G^A[AT]TC 1172 GAATC XcmI 1013 CCACTGGAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1736 CCATCAATGCGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaII BbvI BcnI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII