(Circular) (Six-base) MAPSORT of: pFN21AE1263.seq Check: 1171 from: 1 to: 6526 pFN21AE1263 6526 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4336 278 Size: 53 83 186 936 2800 2468 Fragments arranged by size: 2800 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3869 1937 Size: 1932 4594 Acc65I G'GTAC_C Cuts at: 2932 3847 2932 Size: 915 5611 AclI AA'CG_TT Cuts at: 1563 4653 5026 1563 Size: 3090 373 3063 Fragments arranged by size: 3090 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2510 3613 4589 5973 6241 1632 Size: 878 1103 976 1384 268 1917 Fragments arranged by size: 1917 1384 1103 976 878 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6329 AflIII A'CryG_T Cuts at: 1203 5425 6402 1203 Size: 4222 977 1327 Fragments arranged by size: 4222 1327 977 AgeI A'CCGG_T Cuts at: 5357 5357 Size: 6526 AhdI GACnn_n'nnGTC Cuts at: 2864 5255 2864 Size: 2391 4135 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6526 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3824 1184 Size: 2640 3886 AlwNI CAG_nnn'CTG Cuts at: 2488 2572 2942 3662 4269 5841 2488 Size: 84 370 720 607 1572 3173 Fragments arranged by size: 3173 1572 720 607 370 84 ApaLI G'TGCA_C Cuts at: 3585 4583 5739 3585 Size: 998 1156 4372 Fragments arranged by size: 4372 1156 998 ApoI r'AATT_y Cuts at: 1493 3122 3414 3835 4061 4213 6322 1493 Size: 1629 292 421 226 152 2109 1697 Fragments arranged by size: 2109 1697 1629 421 292 226 152 AseI AT'TA_AT Cuts at: 160 1032 5080 160 Size: 872 4048 1606 Fragments arranged by size: 4048 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6526 AvaI C'yCGr_G Cuts at: 1870 1943 3851 1870 Size: 73 1908 4545 Fragments arranged by size: 4545 1908 73 BamHI G'GATC_C Cuts at: 3350 3856 4223 3350 Size: 506 367 5653 Fragments arranged by size: 5653 506 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2070 2932 3847 5302 618 Size: 325 217 627 283 862 915 1455 1842 Fragments arranged by size: 1842 1455 915 862 627 325 283 217 BanII G_rGCy'C Cuts at: 1391 3513 3674 3845 1391 Size: 2122 161 171 4072 Fragments arranged by size: 4072 2122 171 161 BbeI G_GCGC'C Cuts at: 2074 2074 Size: 6526 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2947 3619 928 Size: 971 1048 672 3835 Fragments arranged by size: 3835 1048 971 672 BbvCI CC'TCA_GC Cuts at: 3666 3666 Size: 6526 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4717 4751 1903 Size: 34 2780 34 3678 Fragments arranged by size: 3678 2780 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2893 4418 5628 2893 Size: 1525 1210 3791 Fragments arranged by size: 3791 1525 1210 BclI T'GATC_A Cuts at: 1548 2749 1548 Size: 1201 5325 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3639 5137 136 Size: 107 122 71 631 605 1967 1498 1525 Fragments arranged by size: 1967 1525 1498 631 605 122 107 71 BglII A'GATC_T Cuts at: 2143 6521 2143 Size: 4378 2148 BlpI GC'TnA_GC Cuts at: 3943 3943 Size: 6526 Bme1580I G_kGCm'C Cuts at: 1790 3589 3820 4587 5743 1790 Size: 1799 231 767 1156 2573 Fragments arranged by size: 2573 1799 1156 767 231 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6526 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2873 4257 5215 Size: 416 53 83 399 24 1456 1384 958 Cuts at: 5215 442 Size: 1753 Fragments arranged by size: 1753 1456 1384 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6526 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2447 2626 2841 3042 3706 5186 1239 Size: 1208 179 215 201 664 1480 2579 Fragments arranged by size: 2579 1480 1208 664 215 201 179 Bpu10I CC'TnA_GC Cuts at: 3515 3666 3515 Size: 151 6375 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4651 5516 5814 4651 Size: 865 298 5363 Fragments arranged by size: 5363 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2756 5189 882 Size: 617 1257 2433 2219 Fragments arranged by size: 2433 2219 1257 617 BsaAI yAC'GTr Cuts at: 493 1204 3649 4317 493 Size: 711 2445 668 2702 Fragments arranged by size: 2702 2445 711 668 BsaBI GATnn'nnATC Cuts at: 2904 3195 6520 2904 Size: 291 3325 2910 Fragments arranged by size: 3325 2910 291 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2071 4333 4715 5350 Size: 53 83 186 936 538 2262 382 635 Cuts at: 5350 275 Size: 1451 Fragments arranged by size: 2262 1451 936 635 538 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3291 4959 5357 5631 5778 6189 3291 Size: 1668 398 274 147 411 3628 Fragments arranged by size: 3628 1668 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2420 2450 1178 Size: 30 165 30 1017 30 5254 Fragments arranged by size: 5254 1017 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 1186 Size: 931 5595 BseYI C'CCAG_C Cuts at: 5729 6225 5729 Size: 496 6030 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3008 3358 1163 Size: 573 1272 350 4331 Fragments arranged by size: 4331 1272 573 350 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3292 3976 4737 5765 1540 Size: 275 186 1291 684 761 1028 2301 Fragments arranged by size: 2301 1291 1028 761 684 275 186 BsiHKAI G_wGCw'C Cuts at: 3513 3589 3696 4587 4672 5743 3513 Size: 76 107 891 85 1071 4296 Fragments arranged by size: 4296 1071 891 107 85 76 BsmI GAATG_Cn' Cuts at: 4037 4130 4037 Size: 93 6433 BsmBI CGTCTCn'nnnn_ Cuts at: 2423 2851 2423 Size: 428 6098 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3513 3589 3674 3696 3820 3845 4587 Size: 399 1723 76 85 22 124 25 742 Cuts at: 4587 4672 5743 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1723 1071 742 399 124 85 85 76 25 22 BspEI T'CCGG_A Cuts at: 6189 6189 Size: 6526 BspHI T'CATG_A Cuts at: 4415 6145 4415 Size: 1730 4796 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2454 3881 844 Size: 1610 1427 3489 Fragments arranged by size: 3489 1610 1427 BsrBI CCG'CTC Cuts at: 1394 1823 4413 1394 Size: 429 2590 3507 Fragments arranged by size: 3507 2590 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3367 3389 5021 5195 65 Size: 1183 2119 22 1632 174 1396 Fragments arranged by size: 2119 1632 1396 1183 174 22 BsrFI r'CCGG_y Cuts at: 1700 1810 2079 5170 5357 1700 Size: 110 269 3091 187 2869 Fragments arranged by size: 3091 2869 269 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6526 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6526 BssSI C'ACGA_G Cuts at: 2204 4586 5598 2204 Size: 2382 1012 3132 Fragments arranged by size: 3132 2382 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2396 3327 3662 794 Size: 1602 931 335 3658 Fragments arranged by size: 3658 1602 931 335 BstBI TT'CG_AA Cuts at: 5330 5330 Size: 6526 BstEII G'GTnAC_C Cuts at: 1184 2575 1184 Size: 1391 5135 BstXI CCAn_nnnn'nTGG Cuts at: 2587 2587 Size: 6526 BstYI r'GATC_y Cuts at: 1972 2143 3137 3310 3350 3856 4223 4608 4625 Size: 171 994 173 40 506 367 385 17 Cuts at: 4625 6077 6163 6175 6521 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 994 506 385 367 346 173 171 86 40 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5974 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5787 Fragments arranged by size: 5787 683 56 BtsI GCAGTG_nn' Cuts at: 4042 4836 4856 4042 Size: 794 20 5712 Fragments arranged by size: 5712 794 20 ClaI AT'CG_AT Cuts at: 1554 3199 1554 Size: 1645 4881 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2293 2328 529 Size: 35 1729 35 4727 Fragments arranged by size: 4727 1729 35 35 DraI TTT'AAA Cuts at: 3830 3830 Size: 6526 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2726 5533 809 Size: 507 1410 2807 1802 Fragments arranged by size: 2807 1802 1410 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3015 3973 4862 Size: 54 1425 50 275 18 1185 958 889 Cuts at: 4862 6491 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1185 958 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3973 1537 Size: 275 2161 4090 Fragments arranged by size: 4090 2161 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2553 2754 2875 4456 1353 Size: 1200 201 121 1581 3423 Fragments arranged by size: 3423 1581 1200 201 121 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5113 5487 5633 387 Size: 1064 138 3524 374 146 1280 Fragments arranged by size: 3524 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3511 3511 Size: 6526 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2447 2510 2626 2841 3042 3613 3706 Size: 393 815 63 116 215 201 571 93 Cuts at: 3706 4589 5186 5973 6241 1239 Size: 883 597 787 268 1524 Fragments arranged by size: 1524 883 815 787 597 571 393 268 215 201 116 93 63 EcoNI CCTnn'n_nnAGG Cuts at: 2820 2820 Size: 6526 EcoRI G'AATT_C Cuts at: 3835 4213 3835 Size: 378 6148 FspI TGC'GCA Cuts at: 2450 5032 2450 Size: 2582 3944 HaeII r_GCGC'y Cuts at: 1678 2050 2074 5673 1678 Size: 372 24 3599 2531 Fragments arranged by size: 3599 2531 372 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2850 2882 3129 3161 3419 Size: 32 135 32 1278 32 247 32 258 Cuts at: 3419 3451 5167 5199 5241 5273 1373 Size: 32 1716 32 42 32 2626 Fragments arranged by size: 2626 1716 1278 258 247 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2980 3870 4116 669 Size: 988 281 1042 890 246 3079 Fragments arranged by size: 3079 1042 988 890 281 246 HindIII A'AGCT_T Cuts at: 748 2456 3044 748 Size: 1708 588 4230 Fragments arranged by size: 4230 1708 588 HpaI GTT'AAC Cuts at: 4116 4116 Size: 6526 KasI G'GCGC_C Cuts at: 2070 2070 Size: 6526 KpnI G_GTAC'C Cuts at: 2936 3851 2936 Size: 915 5611 MfeI C'AATT_G Cuts at: 4125 4125 Size: 6526 MluI A'CGCG_T Cuts at: 6402 6402 Size: 6526 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5640 5824 1305 Size: 4335 184 2007 Fragments arranged by size: 4335 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3017 10 Size: 54 1425 343 1185 3519 Fragments arranged by size: 3519 1425 1185 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4484 4843 5002 518 Size: 648 3318 359 159 2042 Fragments arranged by size: 3318 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2027 2040 2359 3942 4619 5767 6012 1606 Size: 421 13 319 1583 677 1148 245 2120 Fragments arranged by size: 2120 1583 1148 677 421 319 245 13 NarI GG'CG_CC Cuts at: 2071 2071 Size: 6526 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5974 NdeI CA'TA_TG Cuts at: 387 387 Size: 6526 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6526 NotI GC'GGCC_GC Cuts at: 3973 3973 Size: 6526 NspI r_CATG'y Cuts at: 1132 1229 3884 5429 1132 Size: 97 2655 1545 2229 Fragments arranged by size: 2655 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6526 PciI A'CATG_T Cuts at: 5425 5425 Size: 6526 PflMI CCAn_nnn'nTGG Cuts at: 1108 2572 1108 Size: 1464 5062 PfoI T'CCnGG_A Cuts at: 2215 2642 2215 Size: 427 6099 PmeI GTTT'AAAC Cuts at: 3830 3830 Size: 6526 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4595 4627 6108 6140 4595 Size: 32 1481 32 4981 Fragments arranged by size: 4981 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6526 PsiI TTA'TAA Cuts at: 4096 4096 Size: 6526 PstI C_TGCA'G Cuts at: 830 2088 2118 2514 2667 3878 830 Size: 1258 30 396 153 1211 3478 Fragments arranged by size: 3478 1258 1211 396 153 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6526 SacI G_AGCT'C Cuts at: 3513 3513 Size: 6526 SalI G'TCGA_C Cuts at: 1936 3868 1936 Size: 1932 4594 SbfI CC_TGCA'GG Cuts at: 3878 3878 Size: 6526 ScaI AGT'ACT Cuts at: 4774 6486 4774 Size: 1712 4814 SfcI C'TryA_G Cuts at: 826 1045 2084 2114 2171 2510 2663 3874 5009 Size: 219 1039 30 57 339 153 1211 1135 Cuts at: 5009 5690 5881 826 Size: 681 191 1471 Fragments arranged by size: 1471 1211 1135 1039 681 339 219 191 153 57 30 SfoI GGC'GCC Cuts at: 2072 2072 Size: 6526 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6526 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6526 SmaI CCC'GGG Cuts at: 1872 3853 1872 Size: 1981 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4630 5531 5793 820 Size: 197 926 2687 901 262 1553 Fragments arranged by size: 2687 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4317 493 Size: 3824 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6526 SphI G_CATG'C Cuts at: 1132 3884 1132 Size: 2752 3774 SspI AAT'ATT Cuts at: 5 52 4450 5 Size: 47 4398 2081 Fragments arranged by size: 4398 2081 47 StuI AGG'CCT Cuts at: 1517 2826 1517 Size: 1309 5217 StyI C'CwwG_G Cuts at: 513 1065 2529 3965 513 Size: 552 1464 1436 3074 Fragments arranged by size: 3074 1464 1436 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4553 4570 4723 4908 5353 325 Size: 1255 2973 17 153 185 445 1498 Fragments arranged by size: 2973 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4772 6484 96 Size: 274 80 33 51 1443 2795 1712 138 Fragments arranged by size: 2795 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2420 2452 3722 3754 6486 6518 2420 Size: 32 1270 32 2732 32 2428 Fragments arranged by size: 2732 2428 1270 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6526 XbaI T'CTAG_A Cuts at: 3862 3862 Size: 6526 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3025 3748 3025 Size: 723 5803 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6526 XmaI C'CCGG_G Cuts at: 1870 3851 1870 Size: 1981 4545 XmnI GAAnn'nnTTC Cuts at: 3714 4655 3714 Size: 941 5585 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4334 276 Size: 53 83 186 936 2800 2468 Fragments arranged by size: 2800 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI