(Linear) (Six-base) MAPSORT of: KIEE2060.seq Check: 274 from: 1 to: 2115 KIEE2060.orf With 182 enzymes: SgfI * July 15, 2010 18:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1291 1422 2115 Size: 1291 131 693 Fragments arranged by size: 1291 693 131 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 224 529 2115 Size: 224 305 1586 Fragments arranged by size: 1586 305 224 AflIII A'CryG_T Cuts at: 0 1907 2115 Size: 1907 208 AleI CACnn'nnGTG Cuts at: 0 1573 2065 2115 Size: 1573 492 50 Fragments arranged by size: 1573 492 50 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 841 873 2115 Size: 841 32 1242 Fragments arranged by size: 1242 841 32 AlwNI CAG_nnn'CTG Cuts at: 0 489 969 1050 2115 Size: 489 480 81 1065 Fragments arranged by size: 1065 489 480 81 ApaI G_GGCC'C Cuts at: 0 1470 2115 Size: 1470 645 ApoI r'AATT_y Cuts at: 0 310 2115 Size: 310 1805 AvaI C'yCGr_G Cuts at: 0 297 2115 Size: 297 1818 BamHI G'GATC_C Cuts at: 0 465 1487 2115 Size: 465 1022 628 Fragments arranged by size: 1022 628 465 BanI G'GyrC_C Cuts at: 0 21 2115 Size: 21 2094 BanII G_rGCy'C Cuts at: 0 1470 1586 2115 Size: 1470 116 529 Fragments arranged by size: 1470 529 116 BbsI GAAGACnn'nnnn_ Cuts at: 0 168 557 2115 Size: 168 389 1558 Fragments arranged by size: 1558 389 168 BciVI GTATCCnnnnn_n' Cuts at: 0 151 1213 1523 2115 Size: 151 1062 310 592 Fragments arranged by size: 1062 592 310 151 BclI T'GATC_A Cuts at: 0 140 2115 Size: 140 1975 BglI GCCn_nnn'nGGC Cuts at: 0 533 2115 Size: 533 1582 BglII A'GATC_T Cuts at: 0 230 767 2115 Size: 230 537 1348 Fragments arranged by size: 1348 537 230 Bme1580I G_kGCm'C Cuts at: 0 928 1470 2115 Size: 928 542 645 Fragments arranged by size: 928 645 542 BmrI ACTGGGnnnn_n' Cuts at: 0 283 295 471 2115 Size: 283 12 176 1644 Fragments arranged by size: 1644 283 176 12 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 214 600 643 730 1702 2115 Size: 214 386 43 87 972 413 Fragments arranged by size: 972 413 386 214 87 43 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 685 2115 Size: 685 1430 BsaAI yAC'GTr Cuts at: 0 1088 2079 2115 Size: 1088 991 36 Fragments arranged by size: 1088 991 36 BsaHI Gr'CG_yC Cuts at: 0 1943 2115 Size: 1943 172 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 249 279 2115 Size: 249 30 1836 Fragments arranged by size: 1836 249 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 382 475 1708 1813 2115 Size: 382 93 1233 105 302 Fragments arranged by size: 1233 382 302 105 93 BseYI C'CCAG_C Cuts at: 0 470 929 1473 2000 2115 Size: 470 459 544 527 115 Fragments arranged by size: 544 527 470 459 115 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 597 679 1269 2058 2115 Size: 597 82 590 789 57 Fragments arranged by size: 789 597 590 82 57 BsiEI CG_ry'CG Cuts at: 0 1482 2115 Size: 1482 633 BsiHKAI G_wGCw'C Cuts at: 0 1586 2072 2115 Size: 1586 486 43 Fragments arranged by size: 1586 486 43 BsmI GAATG_Cn' Cuts at: 0 558 1053 2115 Size: 558 495 1062 Fragments arranged by size: 1062 558 495 Bsp1286I G_dGCh'C Cuts at: 0 928 1470 1586 2072 2115 Size: 928 542 116 486 43 Fragments arranged by size: 928 542 486 116 43 BspHI T'CATG_A Cuts at: 0 1586 1812 2115 Size: 1586 226 303 Fragments arranged by size: 1586 303 226 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 659 977 1018 1151 1291 1422 2115 Size: 659 318 41 133 140 131 693 Fragments arranged by size: 693 659 318 140 133 131 41 BsrBI CCG'CTC Cuts at: 0 681 2115 Size: 681 1434 BsrDI GCAATG_nn' Cuts at: 0 846 1001 2115 Size: 846 155 1114 Fragments arranged by size: 1114 846 155 BsrFI r'CCGG_y Cuts at: 0 24 2115 Size: 24 2091 BsrGI T'GTAC_A Cuts at: 0 590 1277 2115 Size: 590 687 838 Fragments arranged by size: 838 687 590 BstAPI GCAn_nnn'nTGC Cuts at: 0 2036 2115 Size: 2036 79 BstXI CCAn_nnnn'nTGG Cuts at: 0 1081 1176 2115 Size: 1081 95 939 Fragments arranged by size: 1081 939 95 BstYI r'GATC_y Cuts at: 0 230 465 767 849 1487 2115 Size: 230 235 302 82 638 628 Fragments arranged by size: 638 628 302 235 230 82 Bsu36I CC'TnA_GG Cuts at: 0 1819 2115 Size: 1819 296 BtgI C'CryG_G Cuts at: 0 152 1370 1449 2009 2115 Size: 152 1218 79 560 106 Fragments arranged by size: 1218 560 152 106 79 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1656 2115 Size: 1656 459 BtsI GCAGTG_nn' Cuts at: 0 744 2115 Size: 744 1371 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1107 1142 2115 Size: 1107 35 973 Fragments arranged by size: 1107 973 35 EaeI y'GGCC_r Cuts at: 0 2006 2115 Size: 2006 109 EarI CTCTTCn'nnn_ Cuts at: 0 1574 2115 Size: 1574 541 EcoICRI GAG'CTC Cuts at: 0 1584 2115 Size: 1584 531 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 214 224 529 600 643 730 1702 2115 Size: 214 10 305 71 43 87 972 413 Fragments arranged by size: 972 413 305 214 87 71 43 10 EcoNI CCTnn'n_nnAGG Cuts at: 0 203 2115 Size: 203 1912 EcoO109I rG'GnC_Cy Cuts at: 0 684 815 1466 1467 1708 1983 2115 Size: 684 131 651 1 241 275 132 Fragments arranged by size: 684 651 275 241 132 131 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 192 2115 Size: 192 1923 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 78 110 1100 1132 1882 1914 2115 Size: 78 32 990 32 750 32 201 Fragments arranged by size: 990 750 201 78 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 386 486 2115 Size: 386 100 1629 Fragments arranged by size: 1629 386 100 MscI TGG'CCA Cuts at: 0 2008 2115 Size: 2008 107 MslI CAynn'nnrTG Cuts at: 0 673 1174 1571 1573 2065 2115 Size: 673 501 397 2 492 50 Fragments arranged by size: 673 501 492 397 50 2 MspA1I CmG'CkG Cuts at: 0 1066 1484 1498 2115 Size: 1066 418 14 617 Fragments arranged by size: 1066 617 418 14 NaeI GCC'GGC Cuts at: 0 26 2115 Size: 26 2089 NgoMIV G'CCGG_C Cuts at: 0 24 2115 Size: 24 2091 NspI r_CATG'y Cuts at: 0 1911 2115 Size: 1911 204 PasI CC'CwG_GG Cuts at: 0 1704 2115 Size: 1704 411 PciI A'CATG_T Cuts at: 0 1907 2115 Size: 1907 208 PflMI CCAn_nnn'nTGG Cuts at: 0 420 1351 2115 Size: 420 931 764 Fragments arranged by size: 931 764 420 PfoI T'CCnGG_A Cuts at: 0 442 707 2115 Size: 442 265 1408 Fragments arranged by size: 1408 442 265 PmlI CAC'GTG Cuts at: 0 1088 2115 Size: 1088 1027 PpuMI rG'GwC_Cy Cuts at: 0 1708 1983 2115 Size: 1708 275 132 Fragments arranged by size: 1708 275 132 PshAI GACnn'nnGTC Cuts at: 0 1637 2115 Size: 1637 478 PspOMI G'GGCC_C Cuts at: 0 1466 2115 Size: 1466 649 PstI C_TGCA'G Cuts at: 0 40 974 2115 Size: 40 934 1141 Fragments arranged by size: 1141 934 40 PvuII CAG'CTG Cuts at: 0 1066 1498 2115 Size: 1066 432 617 Fragments arranged by size: 1066 617 432 SacI G_AGCT'C Cuts at: 0 1586 2115 Size: 1586 529 SanDI GG'GwC_CC Cuts at: 0 1708 1983 2115 Size: 1708 275 132 Fragments arranged by size: 1708 275 132 SapI GCTCTTCn'nnn_ Cuts at: 0 1574 2115 Size: 1574 541 ScaI AGT'ACT Cuts at: 0 1210 2115 Size: 1210 905 SexAI A'CCwGG_T Cuts at: 0 1043 2115 Size: 1043 1072 SfcI C'TryA_G Cuts at: 0 36 663 763 970 2115 Size: 36 627 100 207 1145 Fragments arranged by size: 1145 627 207 100 36 SmaI CCC'GGG Cuts at: 0 299 2115 Size: 299 1816 SmlI C'TyrA_G Cuts at: 0 700 2115 Size: 700 1415 StuI AGG'CCT Cuts at: 0 1855 2115 Size: 1855 260 StyI C'CwwG_G Cuts at: 0 321 1517 2115 Size: 321 1196 598 Fragments arranged by size: 1196 598 321 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 623 1940 1992 2115 Size: 623 1317 52 123 Fragments arranged by size: 1317 623 123 52 TatI w'GTAC_w Cuts at: 0 590 741 1208 1277 2115 Size: 590 151 467 69 838 Fragments arranged by size: 838 590 467 151 69 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 149 181 249 281 1497 1529 1692 1724 Size: 149 32 68 32 1216 32 163 32 Cuts at: 1724 2115 Size: 391 Fragments arranged by size: 1216 391 163 149 68 32 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1957 2115 Size: 1957 158 XmaI C'CCGG_G Cuts at: 0 297 2115 Size: 297 1818 Enzymes that do cut: AarI AcuI AflIII AleI AloI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbsI BciVI BclI BglI BglII Bme1580I BmrI BpmI BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I MmeI MscI MslI MspA1I NaeI NgoMIV NspI PasI PciI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SacI SanDI SapI ScaI SexAI SfcI SmaI SmlI StuI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BfrBI BlpI BmgBI BmtI BplI Bpu10I BsaI BsaBI BsaWI BsiWI BsmBI BspEI BssHII BssSI BstBI BstEII BstZ17I ClaI DraI DraIII DrdI EagI EciI EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI NarI NcoI NdeI NheI NotI NruI NsiI PacI PmeI PpiI PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI