################################## # NO CUT ENZYMES ( 197 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmFI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DdeI DpnII DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HaeII HaeIII HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI KspI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MunI MvnI NaeI NarI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AvaI 191 CCCGGG C^[CT]CG[GA]G AviII 222 TGCGCA TGC^GCA BbsI 3 GTCTTC GAAGAC(2/6) BfaI 25 CTAG C^TAG BpuAI 3 GTCTTC GAAGAC(2/6) BsgI 79 GTGCAG GTGCAG(16/14) BsmI 68 GAATGC GAATGC(1/-1) BsoBI 191 CCCGGG C^[CT]CG[GA]G BspMI 225 GCAGGT ACCTGC(4/8) BsrI 149 CCAGT ACTGG(1/-1) BstNI 132 CCAGG CC^[AT]GG CfoI 223 GCGC GCG^C DsaI 254 CCATGG C^C[GA][CT]GG EarI 112 GAAGAG CTCTTC(1/4) EcoNI 10 CCTCCCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRII 132 CCAGG ^CC[AT]GG EcoT14I 254 CCATGG C^C[AT][AT]GG FspI 222 TGCGCA TGC^GCA HhaI 223 GCGC GCG^C HinP1I 223 GCGC G^CGC Ksp632I 112 GAAGAG CTCTTC(1/4) MaeI 25 CTAG C^TAG MaeIII 197 GTGAC ^GT[ACGT]AC MvaI 132 CCAGG CC^[AT]GG NcoI 254 CCATGG C^CATGG NspI 246 GCATGC [GA]CATG^[CT] SfaNI 264 GCATC GCATC(5/9) SmaI 191 CCCGGG CCC^GGG SphI 246 GCATGC GCATG^C StyI 254 CCATGG C^C[AT][AT]GG Tsp45I 197 GTGAC ^GT[GC]AC Tsp509I 75 AATT ^AATT TspRI 165 CACTG CA[GC]TG[ACGT][ACGT]^ XmaCI 191 CCCGGG C^CCGGG XmaI 191 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AluI 36 AGCT AG^CT 60 AGCT BbvI 294 GCAGC GCAGC(8/12) 220 GCTGC BsmAI 28 GAGAC GTCTC(1/5) 101 GAGAC Cac8I 48 GCCCGC GC[ACGT]^[ACGT]GC 246 GCATGC FauI 45 CCCGC CCCGC(4/6) 49 CCCGC HapII 192 CCGG C^CGG 214 CCGG HpaII 192 CCGG C^CGG 214 CCGG HphI 196 GGTGA GGTGA(8/7) 234 TCACC MboII 112 GAAGA GAAGA(8/7) 4 TCTTC MnlI 10 CCTC CCTC(7/6) 251 CCTC MspI 192 CCGG C^CGG 214 CCGG NlaIII 247 CATG CATG^ 255 CATG TseI 220 GCTGC G^C[AT]GC 294 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 13 enzymes) # ######################################### AciI BcnI BsaJI BsiYI BslI BssKI BstF5I Fnu4HI FokI ItaI MwoI NciI ScrFI