################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AviII AvrII BbrPI BbvCI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BspDI BspEI BspHI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII ClaI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV FseI FspI HgaI HpaI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI SacI SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I XbaI XcmI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 636 GTCTAC GT^[AC][GT]AC AcyI 664 GGCGCC G[GA]^CG[CT]C AseI 2098 ATTAAT AT^TAAT AsnI 2098 ATTAAT AT^TAAT BamHI 1432 GGATCC G^GATCC BanII 38 GAGCCC G[GA]GC[CT]^C BbeI 664 GGCGCC GGCGC^C BcgI 147 CGAGCGGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 2108 AGATCT A^GATCT BmyI 38 GAGCCC G[AGT]GC[ACT]^C BsaHI 664 GGCGCC G[GA]^CG[CT]C BsaI 2049 GGTCTC GGTCTC(1/5) BsmBI 1745 GAGACG CGTCTC(1/5) Bsp1286I 38 GAGCCC G[AGT]GC[ACT]^C Bsp1407I 137 TGTACA T^GTACA BspMI 570 ACCTGC ACCTGC(4/8) BsrFI 219 GCCGGT [GA]^CCGG[CT] BsrGI 137 TGTACA T^GTACA BstXI 938 CCACCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 219 GCCGGT [GA]^CCGG[CT] CpoI 163 CGGACCG CG^G[AT]CCG DraI 1240 TTTAAA TTT^AAA EcoNI 447 CCTTGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1282 GAATTC G^AATTC EcoT22I 962 ATGCAT ATGCA^T Hin1I 664 GGCGCC G[GA]^CG[CT]C HincII 976 GTCAAC GT[CT]^[GA]AC HindII 976 GTCAAC GT[CT]^[GA]AC KasI 664 GGCGCC G^GCGCC NarI 664 GGCGCC GG^CGCC NcoI 2036 CCATGG C^CATGG NsiI 962 ATGCAT ATGCA^T PaeR7I 1994 CTCGAG C^TCGAG PleI 2124 GACTC GAGTC(4/5) PshBI 2098 ATTAAT AT^TAAT PstI 873 CTGCAG CTGCA^G RsrII 163 CGGACCG CG^G[AT]CCG ScaI 590 AGTACT AGT^ACT SfcI 873 CTGCAG C^T[GA][CT]AG SfoI 664 GGCGCC GGC^GCC SmaI 41 CCCGGG CCC^GGG SspBI 137 TGTACA T^GTACA SspI 1885 AATATT AAT^ATT XhoI 1994 CTCGAG C^TCGAG XmaCI 41 CCCGGG C^CCGGG XmaI 41 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AlwNI 2088 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG 2122 CAGACTCTG BanI 51 GGTGCC G^G[CT][GA]CC 664 GGCGCC BciVI 672 GGATAC GTATCC(6/5) 1329 GGATAC BclI 86 TGATCA T^GATCA 1973 TGATCA BsmAI 1745 GAGAC GTCTC(1/5) 2050 GTCTC BsmFI 14 GGGAC GGGAC(10/14) 1465 GGGAC BspLU11I 194 ACATGT A^CATGT 1537 ACATGT BsrBI 148 GAGCGG CCGCTC(-3/-3) 160 GAGCGG DraII 1005 AGGTCCT [GA]G^G[ACGT]CC[CT] 1465 GGGACCC DsaI 1795 CCACGG C^C[GA][CT]GG 2036 CCATGG EcoO109I 1005 AGGTCCT [GA]G^G[ACGT]CC[CT] 1465 GGGACCC FauI 95 CCCGC CCCGC(4/6) 2054 CCCGC FbaI 86 TGATCA T^GATCA 1973 TGATCA HindIII 1236 AAGCTT A^AGCTT 1720 AAGCTT PpuMI 1005 AGGTCCT [GA]G^G[AT]CC[CT] 1465 GGGACCC PvuII 605 CAGCTG CAG^CTG 1495 CAGCTG SmlI 1914 CTTGAG C^T[CT][GA]AG 1994 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI Asp700I AvaI AvaII BalI BbsI BbvI BcnI BfaI BpmI BpuAI BsaJI BsgI BsiYI BslI BsmI BsoBI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI