(Linear) (Six-base) MAPSORT of: KIEE1274.seq Check: 4879 from: 1 to: 2133 KIEE1274.orf With 182 enzymes: SgfI * July 15, 2010 18:35 .. AccI GT'mk_AC Cuts at: 0 637 2133 Size: 637 1496 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1139 1244 1672 1765 2104 2133 Size: 1139 105 428 93 339 29 Fragments arranged by size: 1139 428 339 105 93 29 AflIII A'CryG_T Cuts at: 0 194 371 1537 2133 Size: 194 177 1166 596 Fragments arranged by size: 1166 596 194 177 AleI CACnn'nnGTG Cuts at: 0 1225 2133 Size: 1225 908 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 7 39 2133 Size: 7 32 2094 Fragments arranged by size: 2094 32 7 AlwNI CAG_nnn'CTG Cuts at: 0 2093 2127 2133 Size: 2093 34 6 Fragments arranged by size: 2093 34 6 ApoI r'AATT_y Cuts at: 0 204 610 1282 1297 1630 1832 1848 1863 Size: 204 406 672 15 333 202 16 15 Cuts at: 1863 1903 1937 2133 Size: 40 34 196 Fragments arranged by size: 672 406 333 204 202 196 40 34 16 15 15 AseI AT'TA_AT Cuts at: 0 2099 2133 Size: 2099 34 AvaI C'yCGr_G Cuts at: 0 41 1017 1994 2133 Size: 41 976 977 139 Fragments arranged by size: 977 976 139 41 BamHI G'GATC_C Cuts at: 0 1432 2133 Size: 1432 701 BanI G'GyrC_C Cuts at: 0 51 664 2133 Size: 51 613 1469 Fragments arranged by size: 1469 613 51 BanII G_rGCy'C Cuts at: 0 42 2133 Size: 42 2091 BbeI G_GCGC'C Cuts at: 0 668 2133 Size: 668 1465 BbsI GAAGACnn'nnnn_ Cuts at: 0 837 942 1163 1330 2133 Size: 837 105 221 167 803 Fragments arranged by size: 837 803 221 167 105 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 136 170 2133 Size: 136 34 1963 Fragments arranged by size: 1963 136 34 BciVI GTATCCnnnnn_n' Cuts at: 0 666 1323 2133 Size: 666 657 810 Fragments arranged by size: 810 666 657 BclI T'GATC_A Cuts at: 0 86 1973 2133 Size: 86 1887 160 Fragments arranged by size: 1887 160 86 BfrBI ATG'CAT Cuts at: 0 964 2133 Size: 964 1169 BglII A'GATC_T Cuts at: 0 2108 2133 Size: 2108 25 BmgBI CAC'GTC Cuts at: 0 1505 2133 Size: 1505 628 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1629 2133 Size: 1629 504 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 479 1411 1492 1918 2133 Size: 479 932 81 426 215 Fragments arranged by size: 932 479 426 215 81 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1935 2133 Size: 1935 198 BsaI GGTCTCn'nnnn_ Cuts at: 0 2055 2133 Size: 2055 78 BsaHI Gr'CG_yC Cuts at: 0 665 2133 Size: 665 1468 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 760 790 2133 Size: 760 30 1343 Fragments arranged by size: 1343 760 30 BseYI C'CCAG_C Cuts at: 0 1259 2133 Size: 1259 874 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 52 451 2068 2133 Size: 52 399 1617 65 Fragments arranged by size: 1617 399 65 52 BsmI GAATG_Cn' Cuts at: 0 159 964 966 1973 2133 Size: 159 805 2 1007 160 Fragments arranged by size: 1007 805 160 159 2 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1739 2133 Size: 1739 394 Bsp1286I G_dGCh'C Cuts at: 0 42 2133 Size: 42 2091 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 579 2133 Size: 579 1554 BsrBI CCG'CTC Cuts at: 0 150 162 2133 Size: 150 12 1971 Fragments arranged by size: 1971 150 12 BsrFI r'CCGG_y Cuts at: 0 219 2133 Size: 219 1914 BsrGI T'GTAC_A Cuts at: 0 137 2133 Size: 137 1996 BstXI CCAn_nnnn'nTGG Cuts at: 0 945 2133 Size: 945 1188 BstYI r'GATC_y Cuts at: 0 867 1432 1647 2108 2133 Size: 867 565 215 461 25 Fragments arranged by size: 867 565 461 215 25 BtgI C'CryG_G Cuts at: 0 1795 2036 2133 Size: 1795 241 97 Fragments arranged by size: 1795 241 97 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 46 81 2133 Size: 46 35 2052 Fragments arranged by size: 2052 46 35 DraI TTT'AAA Cuts at: 0 1242 2133 Size: 1242 891 EaeI y'GGCC_r Cuts at: 0 1247 1792 2033 2133 Size: 1247 545 241 100 Fragments arranged by size: 1247 545 241 100 EarI CTCTTCn'nnn_ Cuts at: 0 320 1003 1119 2133 Size: 320 683 116 1014 Fragments arranged by size: 1014 683 320 116 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 479 1139 1244 1411 1492 1672 1765 1918 Size: 479 660 105 167 81 180 93 153 Cuts at: 1918 2104 2133 Size: 186 29 Fragments arranged by size: 660 479 186 180 167 153 105 93 81 29 EcoNI CCTnn'n_nnAGG Cuts at: 0 451 2133 Size: 451 1682 EcoO109I rG'GnC_Cy Cuts at: 0 1006 1466 2133 Size: 1006 460 667 Fragments arranged by size: 1006 667 460 EcoRI G'AATT_C Cuts at: 0 1282 2133 Size: 1282 851 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1225 2040 2133 Size: 1225 815 93 Fragments arranged by size: 1225 815 93 HaeII r_GCGC'y Cuts at: 0 414 668 1489 2046 2133 Size: 414 254 821 557 87 Fragments arranged by size: 821 557 414 254 87 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 673 705 1359 1391 2133 Size: 673 32 654 32 742 Fragments arranged by size: 742 673 654 32 32 HincII GTy'rAC Cuts at: 0 978 2133 Size: 978 1155 HindIII A'AGCT_T Cuts at: 0 1236 1720 2133 Size: 1236 484 413 Fragments arranged by size: 1236 484 413 KasI G'GCGC_C Cuts at: 0 664 2133 Size: 664 1469 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 777 2133 Size: 777 1356 MscI TGG'CCA Cuts at: 0 1249 1794 2035 2133 Size: 1249 545 241 98 Fragments arranged by size: 1249 545 241 98 MslI CAynn'nnrTG Cuts at: 0 199 1225 1363 2133 Size: 199 1026 138 770 Fragments arranged by size: 1026 770 199 138 MspA1I CmG'CkG Cuts at: 0 607 1497 2057 2133 Size: 607 890 560 76 Fragments arranged by size: 890 607 560 76 NarI GG'CG_CC Cuts at: 0 665 2133 Size: 665 1468 NcoI C'CATG_G Cuts at: 0 2036 2133 Size: 2036 97 NsiI A_TGCA'T Cuts at: 0 966 2133 Size: 966 1167 NspI r_CATG'y Cuts at: 0 198 910 1541 1768 2133 Size: 198 712 631 227 365 Fragments arranged by size: 712 631 365 227 198 PasI CC'CwG_GG Cuts at: 0 9 2133 Size: 9 2124 PciI A'CATG_T Cuts at: 0 194 1537 2133 Size: 194 1343 596 Fragments arranged by size: 1343 596 194 PflMI CCAn_nnn'nTGG Cuts at: 0 80 222 756 2133 Size: 80 142 534 1377 Fragments arranged by size: 1377 534 142 80 PpuMI rG'GwC_Cy Cuts at: 0 1006 1466 2133 Size: 1006 460 667 Fragments arranged by size: 1006 667 460 PspXI vC'TCGA_Gb Cuts at: 0 1994 2133 Size: 1994 139 PstI C_TGCA'G Cuts at: 0 877 2133 Size: 877 1256 PvuII CAG'CTG Cuts at: 0 607 1497 2133 Size: 607 890 636 Fragments arranged by size: 890 636 607 RsrII CG'GwC_CG Cuts at: 0 164 2133 Size: 164 1969 SanDI GG'GwC_CC Cuts at: 0 1466 2133 Size: 1466 667 ScaI AGT'ACT Cuts at: 0 592 2133 Size: 592 1541 SfcI C'TryA_G Cuts at: 0 873 2133 Size: 873 1260 SfoI GGC'GCC Cuts at: 0 666 2133 Size: 666 1467 SmaI CCC'GGG Cuts at: 0 43 2133 Size: 43 2090 SmlI C'TyrA_G Cuts at: 0 1914 1994 2133 Size: 1914 80 139 Fragments arranged by size: 1914 139 80 SspI AAT'ATT Cuts at: 0 1887 2133 Size: 1887 246 StyI C'CwwG_G Cuts at: 0 74 447 763 1002 2036 2133 Size: 74 373 316 239 1034 97 Fragments arranged by size: 1034 373 316 239 97 74 TatI w'GTAC_w Cuts at: 0 137 590 716 1526 2133 Size: 137 453 126 810 607 Fragments arranged by size: 810 607 453 137 126 XhoI C'TCGA_G Cuts at: 0 1994 2133 Size: 1994 139 XmaI C'CCGG_G Cuts at: 0 41 2133 Size: 41 2092 XmnI GAAnn'nnTTC Cuts at: 0 314 964 1641 1810 1867 2133 Size: 314 650 677 169 57 266 Fragments arranged by size: 677 650 314 266 169 57 Enzymes that do cut: AccI AcuI AflIII AleI AloI AlwNI ApoI AseI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII BmgBI BplI BpmI BpuEI BsaI BsaHI BsaXI BseYI BsgI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BstXI BstYI BtgI CspCI DraI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I NarI NcoI NsiI NspI PasI PciI PflMI PpuMI PspXI PstI PvuII RsrII SanDI ScaI SfcI SfoI SmaI SmlI SspI StyI TatI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI ApaI ApaLI AscI AsiSI AvrII BaeI BbvCI BglI BlpI Bme1580I BmrI BmtI Bpu10I BsaAI BsaBI BsaWI BseRI BsiEI BsiHKAI BsiWI BspEI BspHI BsrDI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI DraIII DrdI EagI EciI EcoICRI EcoRV FseI FspI FspAI HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI PacI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PvuI SacI SacII SalI SapI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI StuI SwaI TaqII TstI Tth111I XbaI XcmI ZraI