################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AlwNI Aor51HI ApoI AscI AseI AsnI Asp718I AspI AviII AvrII BbeI BbrPI BbvCI BciVI BclI BfaI BfrI BglII BlnI BsaAI BsaBI BseAI BsiWI BsmBI BspDI BspEI BspHI BsrDI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DrdI Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AcyI 13 GACGCC G[GA]^CG[CT]C AflIII 1307 ACATGT A^C[GA][CT]GT AhdI 28 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 298 CCTCAGG CC^T[ACGT]AGG AspEI 28 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 1249 GGTCC G^G[AT]CC BalI 1547 TGGCCA TGG^CCA BamHI 1072 GGATCC G^GATCC BcgI 1433 GCAGCTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 16 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 379 GCTGAGC GC^T[ACGT]AGC Bpu1102I 379 GCTGAGC GC^T[ACGT]AGC BsaHI 13 GACGCC G[GA]^CG[CT]C BsaI 82 GAGACC GGTCTC(1/5) BsaWI 1196 ACCGGA [AT]^CCGG[AT] BsgI 1047 CTGCAC GTGCAG(16/14) BsmI 1408 GAATGC GAATGC(1/-1) Bsp1407I 462 TGTACA T^GTACA BspLU11I 1307 ACATGT A^CATGT BspMI 999 GCAGGT ACCTGC(4/8) BsrFI 1525 ACCGGC [GA]^CCGG[CT] BsrGI 462 TGTACA T^GTACA BstAPI 1049 GCACCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 788 CCAGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1072 GGATCC [GA]^GATC[CT] Bsu36I 298 CCTCAGG CC^T[ACGT]AGG CelII 379 GCTGAGC GC^T[ACGT]AGC Cfr10I 1525 ACCGGC [GA]^CCGG[CT] EagI 1527 CGGCCG C^GGCCG Eam1105I 28 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1527 CGGCCG C^GGCCG Eco52I 1527 CGGCCG C^GGCCG Eco81I 298 CCTCAGG CC^T[ACGT]AGG Hin1I 13 GACGCC G[GA]^CG[CT]C MflI 1072 GGATCC [GA]^GATC[CT] MluNI 1547 TGGCCA TGG^CCA MscI 1547 TGGCCA TGG^CCA SacI 675 GAGCTC GAGCT^C SapI 1662 GCTCTTC GCTCTTC(1/4) SphI 905 GCATGC GCATG^C SspBI 462 TGTACA T^GTACA StuI 1280 AGGCCT AGG^CCT TfiI 224 GATTC G^A[AT]TC XcmI 785 CCACCAGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1072 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 36 GTCTAC GT^[AC][GT]AC 349 GTCTAC AfaI 261 GTAC GT^AC 463 GTAC Alw44I 1226 GTGCAC G^TGCAC 1471 GTGCAC AlwI 1073 GATCC GGATC(4/5) 1072 GGATC ApaLI 1226 GTGCAC G^TGCAC 1471 GTGCAC Asp700I 1080 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1647 GAACTGCTTC BbsI 846 GAAGAC GAAGAC(2/6) 1020 GAAGAC BpuAI 846 GAAGAC GAAGAC(2/6) 1020 GAAGAC BsiEI 435 CGACCG CG[GA][CT]^CG 1527 CGGCCG BsrBI 1090 GAGCGG CCGCTC(-3/-3) 1483 GAGCGG BssSI 1264 CACGAG CACGAG(-5/-1) 1480 CACGAG DpnII 1068 GATC ^GATC 1073 GATC DraIII 1229 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG 1374 CACGCTGTG DsaI 1013 CCATGG C^C[GA][CT]GG 1544 CCATGG EaeI 1527 CGGCCG [CT]^GGCC[GA] 1547 TGGCCA EarI 1132 CTCTTC CTCTTC(1/4) 1663 CTCTTC HgaI 13 GACGC GACGC(5/10) 740 GCGTC Ksp632I 1132 CTCTTC CTCTTC(1/4) 1663 CTCTTC MboI 1068 GATC ^GATC 1073 GATC NcoI 1013 CCATGG C^CATGG 1544 CCATGG NdeII 1068 GATC ^GATC 1073 GATC NspI 905 GCATGC [GA]CATG^[CT] 1307 ACATGT PstI 488 CTGCAG CTGCA^G 919 CTGCAG PvuII 377 CAGCTG CAG^CTG 895 CAGCTG RsaI 261 GTAC GT^AC 463 GTAC Sau3AI 1068 GATC ^GATC 1073 GATC SmaI 886 CCCGGG CCC^GGG 1337 CCCGGG XmaCI 886 CCCGGG C^CCGGG 1337 CCCGGG XmaI 886 CCCGGG C^CCGGG 1337 CCCGGG XmnI 1080 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1647 GAACTGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AccII AciI AluI ApaI AspHI AvaI BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PleI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TseI Tsp45I TspRI TthHB8I