Restriction map for
pFN21AE2019
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaHI BsaI BseAI BseRI BsiHKAI
BsiWI BsmBI BsmI BspDI BspEI
BspLU11I BsrDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraII DrdI
DsaI Eam1105I Eco47III Eco81I EcoO109I
EcoO65I EcoRI EcoRV FseI FspI
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MroI MunI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI RsrII SacI
SacII SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspI StuI SwaI Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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ApaI 118 GGGCCC GGGCC^C
AseI 886 ATTAAT AT^TAAT
AsnI 886 ATTAAT AT^TAAT
AvaI 115 CCCGGG C^[CT]CG[GA]G
AvaII 549 GGACC G^G[AT]CC
BanII 118 GGGCCC G[GA]GC[CT]^C
BbsI 1691 GTCTTC GAAGAC(2/6)
BclI 1662 TGATCA T^GATCA
BlpI 1366 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1366 GCTGAGC GC^T[ACGT]AGC
BpuAI 1691 GTCTTC GAAGAC(2/6)
BsaWI 551 ACCGGA [AT]^CCGG[AT]
BsiEI 723 CGGCCG CG[GA][CT]^CG
BsoBI 115 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1715 TGTACA T^GTACA
BspHI 1665 TCATGA T^CATGA
BsrBI 720 GAGCGG CCGCTC(-3/-3)
BsrFI 29 GCCGGT [GA]^CCGG[CT]
BsrGI 1715 TGTACA T^GTACA
BstAPI 616 GCAGGTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1008 CCATGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 233 GGATCT [GA]^GATC[CT]
CelII 1366 GCTGAGC GC^T[ACGT]AGC
Cfr10I 29 GCCGGT [GA]^CCGG[CT]
DraIII 1630 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 723 CGGCCG C^GGCCG
EclXI 723 CGGCCG C^GGCCG
Eco52I 723 CGGCCG C^GGCCG
FbaI 1662 TGATCA T^GATCA
HaeII 1670 AGCGCC [GA]GCGC^[CT]
MflI 233 GGATCT [GA]^GATC[CT]
MspA1I 523 CAGCTG C[AC]G^C[GT]G
NdeI 329 CATATG CA^TATG
PflMI 1629 CCACGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 886 ATTAAT AT^TAAT
PvuII 523 CAGCTG CAG^CTG
RcaI 1665 TCATGA T^CATGA
SapI 1699 GAAGAGC GCTCTTC(1/4)
SexAI 962 ACCTGGT A^CC[AT]GGT
SmaI 115 CCCGGG CCC^GGG
SmlI 810 CTTGAG C^T[CT][GA]AG
SphI 1493 GCATGC GCATG^C
SspBI 1715 TGTACA T^GTACA
Van91I 1629 CCACGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 233 GGATCT [GA]^GATC[CT]
XmaCI 115 CCCGGG C^CCGGG
XmaI 115 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 302 GTCGAC GT^[AC][GT]AC
855 GTCGAC
AccII 56 CGCG CG^CG
58 CGCG
AcsI 406 AAATTT [GA]^AATT[CT]
475 GAATTT
ApoI 406 AAATTT [GA]^AATT[CT]
475 GAATTT
BalI 609 TGGCCA TGG^CCA
1034 TGGCCA
BfaI 697 CTAG C^TAG
1141 CTAG
BmyI 118 GGGCCC G[AGT]GC[ACT]^C
728 GGGCAC
BpmI 745 CTCCAG CTGGAG(16/14)
986 CTGGAG
BsgI 560 GTGCAG GTGCAG(16/14)
1069 GTGCAG
Bsp1286I 118 GGGCCC G[AGT]GC[ACT]^C
728 GGGCAC
BspMI 568 ACCTGC ACCTGC(4/8)
616 GCAGGT
BstNI 633 CCAGG CC^[AT]GG
963 CCTGG
BstUI 56 CGCG CG^CG
58 CGCG
EarI 1075 GAAGAG CTCTTC(1/4)
1699 GAAGAG
EcoNI 338 CCTTTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
893 CCTTCAAGAGG
EcoRII 633 CCAGG ^CC[AT]GG
963 CCTGG
FauI 54 CCCGC CCCGC(4/6)
59 GCGGG
Ksp632I 1075 GAAGAG CTCTTC(1/4)
1699 GAAGAG
MaeI 697 CTAG C^TAG
1141 CTAG
MluNI 609 TGGCCA TGG^CCA
1034 TGGCCA
MscI 609 TGGCCA TGG^CCA
1034 TGGCCA
MslI 760 CACCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1009 CATGTGGATG
MvaI 633 CCAGG CC^[AT]GG
963 CCTGG
MvnI 56 CGCG CG^CG
58 CGCG
NspI 247 GCATGT [GA]CATG^[CT]
1493 GCATGC
SalI 302 GTCGAC G^TCGAC
855 GTCGAC
TfiI 134 GAATC G^A[AT]TC
1285 GAATC
Tsp45I 1099 GTCAC ^GT[GC]AC
1534 GTGAC
XcmI 349 CCAAAGGAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
992 CCACTGCTTGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BcnI BsaJI BsiYI BslI BsmAI
BsmFI BsrI BssKI BstF5I Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MseI MspI
MwoI NciI NdeII NlaIII NlaIV
NsiI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I