(Circular) (Six-base) MAPSORT of: pFN21AE2019.seq Check: 9364 from: 1 to: 6493 pFN21AE2019 6493 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4303 278 Size: 53 83 186 936 2767 2468 Fragments arranged by size: 2767 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2308 2861 3836 1937 Size: 371 553 975 4594 Fragments arranged by size: 4594 975 553 371 Acc65I G'GTAC_C Cuts at: 3814 3814 Size: 6493 AclI AA'CG_TT Cuts at: 1563 4620 4993 1563 Size: 3057 373 3063 Fragments arranged by size: 3063 3057 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3723 4556 5940 6208 1632 Size: 2091 833 1384 268 1917 Fragments arranged by size: 2091 1917 1384 833 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6296 AflIII A'CryG_T Cuts at: 1203 5392 6369 1203 Size: 4189 977 1327 Fragments arranged by size: 4189 1327 977 AgeI A'CCGG_T Cuts at: 5324 5324 Size: 6493 AhdI GACnn_n'nnGTC Cuts at: 5222 5222 Size: 6493 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6493 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2275 2546 2590 3080 1184 Size: 1091 271 44 490 4597 Fragments arranged by size: 4597 1091 490 271 44 AlwNI CAG_nnn'CTG Cuts at: 4236 5808 4236 Size: 1572 4921 ApaI G_GGCC'C Cuts at: 2127 2127 Size: 6493 ApaLI G'TGCA_C Cuts at: 4550 5706 4550 Size: 1156 5337 ApoI r'AATT_y Cuts at: 1493 2411 2480 3802 4028 4180 6289 1493 Size: 918 69 1322 226 152 2109 1697 Fragments arranged by size: 2109 1697 1322 918 226 152 69 AseI AT'TA_AT Cuts at: 160 1032 2892 5047 160 Size: 872 1860 2155 1606 Fragments arranged by size: 2155 1860 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6493 AvaI C'yCGr_G Cuts at: 1870 1943 2120 3818 1870 Size: 73 177 1698 4545 Fragments arranged by size: 4545 1698 177 73 BamHI G'GATC_C Cuts at: 3823 4190 3823 Size: 367 6126 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3814 5269 618 Size: 325 217 627 2027 1455 1842 Fragments arranged by size: 2027 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2127 3812 1391 Size: 736 1685 4072 Fragments arranged by size: 4072 1685 736 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3689 928 Size: 971 1790 3732 Fragments arranged by size: 3732 1790 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4684 4718 1903 Size: 34 2747 34 3678 Fragments arranged by size: 3678 2747 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4385 5595 4385 Size: 1210 5283 BclI T'GATC_A Cuts at: 1548 3667 1548 Size: 2119 4374 BfrBI ATG'CAT Cuts at: 3454 3502 3556 3454 Size: 48 54 6391 Fragments arranged by size: 6391 54 48 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5104 136 Size: 107 122 71 631 605 3432 1525 Fragments arranged by size: 3432 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6488 6488 Size: 6493 BlpI GC'TnA_GC Cuts at: 3372 3910 3372 Size: 538 5955 Bme1580I G_kGCm'C Cuts at: 1790 2127 2737 4554 5710 1790 Size: 337 610 1817 1156 2573 Fragments arranged by size: 2573 1817 1156 610 337 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6493 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2138 4224 5182 Size: 416 53 83 399 24 721 2086 958 Cuts at: 5182 442 Size: 1753 Fragments arranged by size: 2086 1753 958 721 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6493 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3106 3697 3106 Size: 591 5902 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2735 3012 5153 1239 Size: 1496 277 2141 2579 Fragments arranged by size: 2579 2141 1496 277 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2836 4618 5483 5781 2836 Size: 1782 865 298 3548 Fragments arranged by size: 3548 1782 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5156 882 Size: 617 3657 2219 Fragments arranged by size: 3657 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4284 493 Size: 711 3080 2702 Fragments arranged by size: 3080 2702 711 BsaBI GATnn'nnATC Cuts at: 6487 6487 Size: 6493 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4300 4682 5317 275 Size: 53 83 186 936 2767 382 635 1451 Fragments arranged by size: 2767 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2556 4926 5324 5598 5745 6156 2556 Size: 2370 398 274 147 411 2893 Fragments arranged by size: 2893 2370 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2095 2125 2351 2381 2733 Size: 30 165 30 692 30 226 30 352 Cuts at: 2733 2763 1178 Size: 30 4908 Fragments arranged by size: 4908 692 352 226 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6493 BseYI C'CCAG_C Cuts at: 3067 5696 6192 3067 Size: 2629 496 3368 Fragments arranged by size: 3368 2629 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2586 3095 1163 Size: 573 850 509 4561 Fragments arranged by size: 4561 850 573 509 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2731 3943 4704 5732 1540 Size: 275 186 730 1212 761 1028 2301 Fragments arranged by size: 2301 1212 1028 761 730 275 186 BsiHKAI G_wGCw'C Cuts at: 4554 4639 5710 4554 Size: 85 1071 5337 Fragments arranged by size: 5337 1071 85 BsmI GAATG_Cn' Cuts at: 4004 4097 4004 Size: 93 6400 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2127 2737 3812 4554 4639 5710 1391 Size: 399 337 610 1075 742 85 1071 2174 Fragments arranged by size: 2174 1075 1071 742 610 399 337 85 BspEI T'CCGG_A Cuts at: 6156 6156 Size: 6493 BspHI T'CATG_A Cuts at: 3670 4382 6112 3670 Size: 712 1730 4051 Fragments arranged by size: 4051 1730 712 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2582 2612 3848 844 Size: 1738 30 1236 3489 Fragments arranged by size: 3489 1738 1236 30 BsrBI CCG'CTC Cuts at: 1394 1823 2727 4380 1394 Size: 429 904 1653 3507 Fragments arranged by size: 3507 1653 904 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4988 5162 65 Size: 1183 3740 174 1396 Fragments arranged by size: 3740 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2034 5137 5324 1700 Size: 110 224 3103 187 2869 Fragments arranged by size: 3103 2869 224 187 110 BsrGI T'GTAC_A Cuts at: 96 3720 96 Size: 3624 2869 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6493 BssSI C'ACGA_G Cuts at: 4553 5565 4553 Size: 1012 5481 BstAPI GCAn_nnn'nTGC Cuts at: 794 2627 794 Size: 1833 4660 BstBI TT'CG_AA Cuts at: 5297 5297 Size: 6493 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6493 BstXI CCAn_nnnn'nTGG Cuts at: 3020 3020 Size: 6493 BstYI r'GATC_y Cuts at: 1972 2238 3823 4190 4575 4592 6044 6130 6142 Size: 266 1585 367 385 17 1452 86 12 Cuts at: 6142 6488 1972 Size: 346 1977 Fragments arranged by size: 1977 1585 1452 385 367 346 266 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5941 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5754 Fragments arranged by size: 5754 683 56 BtsI GCAGTG_nn' Cuts at: 2247 2724 2735 2997 3366 4009 4803 4823 2247 Size: 477 11 262 369 643 794 20 3917 Fragments arranged by size: 3917 794 643 477 369 262 20 11 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6493 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6458 DraI TTT'AAA Cuts at: 3797 3797 Size: 6493 DraIII CAC_nnn'GTG Cuts at: 3640 3640 Size: 6493 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5500 809 Size: 507 4184 1802 Fragments arranged by size: 4184 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2614 2728 3039 Size: 54 1425 50 275 18 784 114 311 Cuts at: 3039 3940 4829 6458 8 Size: 901 889 1629 43 Fragments arranged by size: 1629 1425 901 889 784 311 275 114 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2728 3940 1537 Size: 275 916 1212 4090 Fragments arranged by size: 4090 1212 916 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3075 3699 4423 1353 Size: 1722 624 724 3423 Fragments arranged by size: 3423 1722 724 624 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5080 5454 5600 387 Size: 1064 138 3491 374 146 1280 Fragments arranged by size: 3491 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2735 3012 3723 4556 5153 5940 6208 Size: 393 1103 277 711 833 597 787 268 Cuts at: 6208 1239 Size: 1524 Fragments arranged by size: 1524 1103 833 787 711 597 393 277 268 EcoNI CCTnn'n_nnAGG Cuts at: 2347 2902 2347 Size: 555 5938 EcoRI G'AATT_C Cuts at: 3802 4180 3802 Size: 378 6115 FspI TGC'GCA Cuts at: 4999 4999 Size: 6493 HaeII r_GCGC'y Cuts at: 1678 3679 5640 1678 Size: 2001 1961 2531 Fragments arranged by size: 2531 2001 1961 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3652 3684 5134 5166 5208 Size: 32 135 32 2080 32 1450 32 42 Cuts at: 5208 5240 1373 Size: 32 2626 Fragments arranged by size: 2626 2080 1450 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2309 2862 3028 3837 4083 669 Size: 988 281 371 553 166 809 246 3079 Fragments arranged by size: 3079 988 809 553 371 281 246 166 HindIII A'AGCT_T Cuts at: 748 748 Size: 6493 HpaI GTT'AAC Cuts at: 4083 4083 Size: 6493 KpnI G_GTAC'C Cuts at: 3818 3818 Size: 6493 MfeI C'AATT_G Cuts at: 4092 4092 Size: 6493 MluI A'CGCG_T Cuts at: 6369 6369 Size: 6493 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5607 5791 1305 Size: 4302 184 2007 Fragments arranged by size: 4302 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2616 3041 10 Size: 54 1425 343 784 425 3462 Fragments arranged by size: 3462 1425 784 425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2769 3018 4451 4810 4969 518 Size: 648 1603 249 1433 359 159 2042 Fragments arranged by size: 2042 1603 1433 648 359 249 159 MspA1I CmG'CkG Cuts at: 1606 2530 3909 4586 5734 5979 1606 Size: 924 1379 677 1148 245 2120 Fragments arranged by size: 2120 1379 1148 924 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5941 NdeI CA'TA_TG Cuts at: 387 2335 387 Size: 1948 4545 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6493 NotI GC'GGCC_GC Cuts at: 3940 3940 Size: 6493 NsiI A_TGCA'T Cuts at: 3456 3504 3558 3456 Size: 48 54 6391 Fragments arranged by size: 6391 54 48 NspI r_CATG'y Cuts at: 1132 1229 2256 3502 3851 5396 1132 Size: 97 1027 1246 349 1545 2229 Fragments arranged by size: 2229 1545 1246 1027 349 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6493 PciI A'CATG_T Cuts at: 5392 5392 Size: 6493 PflMI CCAn_nnn'nTGG Cuts at: 1108 3640 1108 Size: 2532 3961 PmeI GTTT'AAAC Cuts at: 3797 3797 Size: 6493 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4562 4594 6075 6107 4562 Size: 32 1481 32 4948 Fragments arranged by size: 4948 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6493 PsiI TTA'TAA Cuts at: 4063 4063 Size: 6493 PspOMI G'GGCC_C Cuts at: 2123 2123 Size: 6493 PstI C_TGCA'G Cuts at: 830 3845 830 Size: 3015 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6493 PvuII CAG'CTG Cuts at: 2530 2530 Size: 6493 SalI G'TCGA_C Cuts at: 1936 2307 2860 3835 1936 Size: 371 553 975 4594 Fragments arranged by size: 4594 975 553 371 SapI GCTCTTCn'nnn_ Cuts at: 3699 3699 Size: 6493 SbfI CC_TGCA'GG Cuts at: 3845 3845 Size: 6493 ScaI AGT'ACT Cuts at: 4741 6453 4741 Size: 1712 4781 SexAI A'CCwGG_T Cuts at: 2967 2967 Size: 6493 SfcI C'TryA_G Cuts at: 826 1045 3841 4976 5657 5848 826 Size: 219 2796 1135 681 191 1471 Fragments arranged by size: 2796 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6493 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6493 SmaI CCC'GGG Cuts at: 1872 2122 3820 1872 Size: 250 1698 4545 Fragments arranged by size: 4545 1698 250 SmlI C'TyrA_G Cuts at: 820 1017 1943 2815 4597 5498 5760 820 Size: 197 926 872 1782 901 262 1553 Fragments arranged by size: 1782 1553 926 901 872 262 197 SnaBI TAC'GTA Cuts at: 493 4284 493 Size: 3791 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6493 SphI G_CATG'C Cuts at: 1132 3502 3851 1132 Size: 2370 349 3774 Fragments arranged by size: 3774 2370 349 SspI AAT'ATT Cuts at: 5 52 4417 5 Size: 47 4365 2081 Fragments arranged by size: 4365 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6493 StyI C'CwwG_G Cuts at: 513 1065 2380 2509 2925 3932 513 Size: 552 1315 129 416 1007 3074 Fragments arranged by size: 3074 1315 1007 552 416 129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2393 2560 3059 3470 4520 4537 4690 Size: 1255 813 167 499 411 1050 17 153 Cuts at: 4690 4875 5320 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1050 813 499 445 411 185 167 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2373 2802 3720 Size: 274 80 33 51 1443 396 429 918 Cuts at: 3720 4739 6451 96 Size: 1019 1712 138 Fragments arranged by size: 1712 1443 1019 918 429 396 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2095 2127 2311 2343 2351 2383 3466 3498 6453 Size: 32 184 32 8 32 1083 32 2955 Cuts at: 6453 6485 2095 Size: 32 2103 Fragments arranged by size: 2955 2103 1083 184 32 32 32 32 32 8 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6493 XbaI T'CTAG_A Cuts at: 3829 3829 Size: 6493 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2361 3004 2361 Size: 643 5850 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6493 XmaI C'CCGG_G Cuts at: 1870 2120 3818 1870 Size: 250 1698 4545 Fragments arranged by size: 4545 1698 250 XmnI GAAnn'nnTTC Cuts at: 4622 4622 Size: 6493 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4301 276 Size: 53 83 186 936 2767 2468 Fragments arranged by size: 2767 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI