Restriction map for
pFN21AE2019
(Linear) (Six-base) MAPSORT of: KIEE2019.seq Check: 5528 from: 1 to: 1788
KIEE2019.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 0 303 856 1788
Size: 303 553 932
Fragments arranged by size:
932 553 303
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1718 1788
Size: 1718 70
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 270 541 585 1075 1788
Size: 270 271 44 490 713
Fragments arranged by size:
713 490 271 270 44
ApaI G_GGCC'C
Cuts at: 0 122 1788
Size: 122 1666
ApoI r'AATT_y
Cuts at: 0 406 475 1788
Size: 406 69 1313
Fragments arranged by size:
1313 406 69
AseI AT'TA_AT
Cuts at: 0 887 1788
Size: 887 901
AvaI C'yCGr_G
Cuts at: 0 115 1788
Size: 115 1673
BanII G_rGCy'C
Cuts at: 0 122 1788
Size: 122 1666
BbsI GAAGACnn'nnnn_
Cuts at: 0 1684 1788
Size: 1684 104
BclI T'GATC_A
Cuts at: 0 1662 1788
Size: 1662 126
BfrBI ATG'CAT
Cuts at: 0 1449 1497 1551 1788
Size: 1449 48 54 237
Fragments arranged by size:
1449 237 54 48
BlpI GC'TnA_GC
Cuts at: 0 1367 1788
Size: 1367 421
Bme1580I G_kGCm'C
Cuts at: 0 122 732 1788
Size: 122 610 1056
Fragments arranged by size:
1056 610 122
BmrI ACTGGGnnnn_n'
Cuts at: 0 133 1788
Size: 133 1655
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1101 1692 1788
Size: 1101 591 96
Fragments arranged by size:
1101 591 96
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 1007 1788
Size: 730 277 781
Fragments arranged by size:
781 730 277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 831 1788
Size: 831 957
BsaWI w'CCGG_w
Cuts at: 0 551 1788
Size: 551 1237
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 90 120 346 376 728 758 1788
Size: 90 30 226 30 352 30 1030
Fragments arranged by size:
1030 352 226 90 30 30 30
BseYI C'CCAG_C
Cuts at: 0 1062 1788
Size: 1062 726
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 1090 1788
Size: 581 509 698
Fragments arranged by size:
698 581 509
BsiEI CG_ry'CG
Cuts at: 0 726 1788
Size: 726 1062
Bsp1286I G_dGCh'C
Cuts at: 0 122 732 1788
Size: 122 610 1056
Fragments arranged by size:
1056 610 122
BspHI T'CATG_A
Cuts at: 0 1665 1788
Size: 1665 123
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 577 607 1788
Size: 577 30 1181
Fragments arranged by size:
1181 577 30
BsrBI CCG'CTC
Cuts at: 0 722 1788
Size: 722 1066
BsrFI r'CCGG_y
Cuts at: 0 29 1788
Size: 29 1759
BsrGI T'GTAC_A
Cuts at: 0 1715 1788
Size: 1715 73
BstAPI GCAn_nnn'nTGC
Cuts at: 0 622 1788
Size: 622 1166
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1015 1788
Size: 1015 773
BstYI r'GATC_y
Cuts at: 0 233 1788
Size: 233 1555
BtsI GCAGTG_nn'
Cuts at: 0 242 719 730 992 1361 1788
Size: 242 477 11 262 369 427
Fragments arranged by size:
477 427 369 262 242 11
DraIII CAC_nnn'GTG
Cuts at: 0 1635 1788
Size: 1635 153
EaeI y'GGCC_r
Cuts at: 0 609 723 1034 1788
Size: 609 114 311 754
Fragments arranged by size:
754 609 311 114
EagI C'GGCC_G
Cuts at: 0 723 1788
Size: 723 1065
EarI CTCTTCn'nnn_
Cuts at: 0 1070 1694 1788
Size: 1070 624 94
Fragments arranged by size:
1070 624 94
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 1007 1718 1788
Size: 730 277 711 70
Fragments arranged by size:
730 711 277 70
EcoNI CCTnn'n_nnAGG
Cuts at: 0 342 897 1788
Size: 342 555 891
Fragments arranged by size:
891 555 342
HaeII r_GCGC'y
Cuts at: 0 1674 1788
Size: 1674 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1647 1679 1788
Size: 1647 32 109
Fragments arranged by size:
1647 109 32
HincII GTy'rAC
Cuts at: 0 304 857 1023 1788
Size: 304 553 166 765
Fragments arranged by size:
765 553 304 166
MscI TGG'CCA
Cuts at: 0 611 1036 1788
Size: 611 425 752
Fragments arranged by size:
752 611 425
MslI CAynn'nnrTG
Cuts at: 0 764 1013 1788
Size: 764 249 775
Fragments arranged by size:
775 764 249
MspA1I CmG'CkG
Cuts at: 0 525 1788
Size: 525 1263
NdeI CA'TA_TG
Cuts at: 0 330 1788
Size: 330 1458
NsiI A_TGCA'T
Cuts at: 0 1451 1499 1553 1788
Size: 1451 48 54 235
Fragments arranged by size:
1451 235 54 48
NspI r_CATG'y
Cuts at: 0 251 1497 1788
Size: 251 1246 291
Fragments arranged by size:
1246 291 251
PflMI CCAn_nnn'nTGG
Cuts at: 0 1635 1788
Size: 1635 153
PspOMI G'GGCC_C
Cuts at: 0 118 1788
Size: 118 1670
PvuII CAG'CTG
Cuts at: 0 525 1788
Size: 525 1263
SalI G'TCGA_C
Cuts at: 0 302 855 1788
Size: 302 553 933
Fragments arranged by size:
933 553 302
SapI GCTCTTCn'nnn_
Cuts at: 0 1694 1788
Size: 1694 94
SexAI A'CCwGG_T
Cuts at: 0 962 1788
Size: 962 826
SmaI CCC'GGG
Cuts at: 0 117 1788
Size: 117 1671
SmlI C'TyrA_G
Cuts at: 0 810 1788
Size: 810 978
SphI G_CATG'C
Cuts at: 0 1497 1788
Size: 1497 291
StyI C'CwwG_G
Cuts at: 0 375 504 920 1788
Size: 375 129 416 868
Fragments arranged by size:
868 416 375 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 388 555 1054 1465 1788
Size: 388 167 499 411 323
Fragments arranged by size:
499 411 388 323 167
TatI w'GTAC_w
Cuts at: 0 368 797 1715 1788
Size: 368 429 918 73
Fragments arranged by size:
918 429 368 73
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 90 122 306 338 346 378 1461 1493
Size: 90 32 184 32 8 32 1083 32
Cuts at: 1493 1788
Size: 295
Fragments arranged by size:
1083 295 184 90 32 32 32 32
8
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 356 999 1788
Size: 356 643 789
Fragments arranged by size:
789 643 356
XmaI C'CCGG_G
Cuts at: 0 115 1788
Size: 115 1673
Enzymes that do cut:
AccI AcuI AlfI ApaI ApoI AseI AvaI BanII
BbsI BclI BfrBI BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaWI BsaXI BseYI BsgI BsiEI Bsp1286I BspHI
BspMI BsrBI BsrFI BsrGI BstAPI BstXI BstYI BtsI
DraIII EaeI EagI EarI Eco57MI EcoNI HaeII Hin4I
HincII MscI MslI MspA1I NdeI NsiI NspI PflMI
PspOMI PvuII SalI SapI SexAI SmaI SmlI SphI
StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaLI AscI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BglI
BglII BmgBI BmtI Bpu10I BsaI BsaAI BsaBI BsaHI
BseRI BsiHKAI BsiWI BsmI BsmBI BspEI BsrDI BssHII
BssSI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DrdI EciI EcoICRI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacI SacII SanDI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI StuI
SwaI Tth111I XbaI XhoI XmnI ZraI