Restriction map for
pFN21AE1216
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BcgI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MseI MslI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI ScaI SfaNI SfoI
SfuI SgrAI SnaBI SpeI SspBI
SspI StuI SwaI TaqI Tru9I
Tsp509I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AlwI 452 GGATC GGATC(4/5)
AlwNI 194 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 216 GGGCCC GGGCC^C
AvaI 74 CCCGGG C^[CT]CG[GA]G
BbvCI 159 CCTCAGC CCTCAGC(-5/-2)
BbvI 139 GCAGC GCAGC(8/12)
BciVI 43 GGATAC GTATCC(6/5)
BfaI 259 CTAG C^TAG
BglI 218 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 166 CTCCAG CTGGAG(16/14)
BsaWI 86 TCCGGT [AT]^CCGG[AT]
BseRI 157 CTCCTC GAGGAG(10/8)
BsmFI 96 GTCCC GGGAC(10/14)
BsoBI 74 CCCGGG C^[CT]CG[GA]G
BsrBI 393 GAGCGG CCGCTC(-3/-3)
BsrDI 298 GCAATG GCAATG(2/0)
BssSI 5 CTCGTG CACGAG(-5/-1)
BstF5I 418 CATCC GGATG(2/0)
BstYI 452 GGATCT [GA]^GATC[CT]
DraIII 274 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
FokI 418 CATCC GGATG(9/13)
HinfI 51 GAATC G^A[ACGT]TC
HphI 206 TCACC GGTGA(8/7)
MaeI 259 CTAG C^TAG
MaeIII 196 GTCAC ^GT[ACGT]AC
MflI 452 GGATCT [GA]^GATC[CT]
MspA1I 469 CCGCTG C[AC]G^C[GT]G
NcoI 228 CCATGG C^CATGG
NspI 184 GCATGC [GA]CATG^[CT]
PpuMI 368 AGGACCC [GA]G^G[AT]CC[CT]
PstI 221 CTGCAG CTGCA^G
SbfI 220 CCTGCAGG CCTGCA^GG
SexAI 275 ACCTGGT A^CC[AT]GGT
SfcI 221 CTGCAG C^T[GA][CT]AG
SfiI 217 GGCCCTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 74 CCCGGG CCC^GGG
SmlI 28 CTCAAG C^T[CT][GA]AG
SphI 184 GCATGC GCATG^C
Sse8387I 220 CCTGCAGG CCTGCA^GG
TfiI 51 GAATC G^A[AT]TC
TseI 139 GCAGC G^C[AT]GC
Tsp45I 196 GTCAC ^GT[GC]AC
XhoII 452 GGATCT [GA]^GATC[CT]
XmaCI 74 CCCGGG C^CCGGG
XmaI 74 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AluI 155 AGCT AG^CT
240 AGCT
BanI 64 GGTGCC G^G[CT][GA]CC
279 GGTGCC
BanII 82 GGGCTC G[GA]GC[CT]^C
216 GGGCCC
BbsI 474 GAAGAC GAAGAC(2/6)
90 GTCTTC
BcnI 74 CCCGG CC^[GC]GG
75 CCGGG
BmyI 82 GGGCTC G[AGT]GC[ACT]^C
216 GGGCCC
BpuAI 474 GAAGAC GAAGAC(2/6)
90 GTCTTC
Bsp1286I 82 GGGCTC G[AGT]GC[ACT]^C
216 GGGCCC
BstNI 276 CCTGG CC^[AT]GG
333 CCAGG
DpnII 202 GATC ^GATC
453 GATC
DsaI 101 CCGTGG C^C[GA][CT]GG
228 CCATGG
EcoRII 276 CCTGG ^CC[AT]GG
333 CCAGG
MboI 202 GATC ^GATC
453 GATC
MboII 474 GAAGA GAAGA(8/7)
91 TCTTC
MvaI 276 CCTGG CC^[AT]GG
333 CCAGG
NciI 74 CCCGG CC^[GC]GG
75 CCGGG
NdeII 202 GATC ^GATC
453 GATC
Sau3AI 202 GATC ^GATC
453 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AvaII BsaJI BsiYI BslI
BsrI BssKI Cac8I Cfr13I DdeI
DraII EcoO109I EcoT14I Fnu4HI HaeIII
HapII HpaII ItaI MnlI MspI
MwoI NlaIII NlaIV Sau96I ScrFI
StyI TspRI