(Circular) (Six-base) MAPSORT of: pFN21AE1216.seq Check: 7961 from: 1 to: 5242 pFN21AE1216 5242 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3052 278 Size: 53 83 186 936 1516 2468 Fragments arranged by size: 2468 1516 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2585 1937 Size: 648 4594 Acc65I G'GTAC_C Cuts at: 2563 2563 Size: 5242 AclI AA'CG_TT Cuts at: 1563 3369 3742 1563 Size: 1806 373 3063 Fragments arranged by size: 3063 1806 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2498 3305 4689 4957 1632 Size: 866 807 1384 268 1917 Fragments arranged by size: 1917 1384 866 807 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5045 AflIII A'CryG_T Cuts at: 1203 4141 5118 1203 Size: 2938 977 1327 Fragments arranged by size: 2938 1327 977 AgeI A'CCGG_T Cuts at: 4073 4073 Size: 5242 AhdI GACnn_n'nnGTC Cuts at: 3971 3971 Size: 5242 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5242 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5242 AlwNI CAG_nnn'CTG Cuts at: 2204 2985 4557 2204 Size: 781 1572 2889 Fragments arranged by size: 2889 1572 781 ApaI G_GGCC'C Cuts at: 2225 2225 Size: 5242 ApaLI G'TGCA_C Cuts at: 3299 4455 3299 Size: 1156 4086 ApoI r'AATT_y Cuts at: 1493 2551 2777 2929 5038 1493 Size: 1058 226 152 2109 1697 Fragments arranged by size: 2109 1697 1058 226 152 AseI AT'TA_AT Cuts at: 160 1032 3796 160 Size: 872 2764 1606 Fragments arranged by size: 2764 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5242 AvaI C'yCGr_G Cuts at: 1870 1943 2079 2567 1870 Size: 73 136 488 4545 Fragments arranged by size: 4545 488 136 73 BamHI G'GATC_C Cuts at: 2572 2939 2572 Size: 367 4875 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2069 2284 2563 4018 618 Size: 325 217 627 282 215 279 1455 1842 Fragments arranged by size: 1842 1455 627 325 282 279 217 215 BanII G_rGCy'C Cuts at: 1391 2091 2225 2561 1391 Size: 700 134 336 4072 Fragments arranged by size: 4072 700 336 134 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2088 2486 928 Size: 971 189 398 3684 Fragments arranged by size: 3684 971 398 189 BbvCI CC'TCA_GC Cuts at: 2165 2165 Size: 5242 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3433 3467 1903 Size: 34 50 34 1412 34 3678 Fragments arranged by size: 3678 1412 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2042 3134 4344 2042 Size: 1092 1210 2940 Fragments arranged by size: 2940 1210 1092 BclI T'GATC_A Cuts at: 1548 1548 Size: 5242 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2229 3853 136 Size: 107 122 71 631 605 557 1624 1525 Fragments arranged by size: 1624 1525 631 605 557 122 107 71 BglII A'GATC_T Cuts at: 5237 5237 Size: 5242 BlpI GC'TnA_GC Cuts at: 2659 2659 Size: 5242 Bme1580I G_kGCm'C Cuts at: 1790 2225 3303 4459 1790 Size: 435 1078 1156 2573 Fragments arranged by size: 2573 1156 1078 435 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5242 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2227 2973 3931 Size: 416 53 83 399 24 810 746 958 Cuts at: 3931 442 Size: 1753 Fragments arranged by size: 1753 958 810 746 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5242 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 3902 1239 Size: 917 1746 2579 Fragments arranged by size: 2579 1746 917 Bpu10I CC'TnA_GC Cuts at: 2165 2432 2165 Size: 267 4975 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2018 3367 4232 4530 2018 Size: 1349 865 298 2730 Fragments arranged by size: 2730 1349 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3905 882 Size: 617 2406 2219 Fragments arranged by size: 2406 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3033 493 Size: 711 1829 2702 Fragments arranged by size: 2702 1829 711 BsaBI GATnn'nnATC Cuts at: 5236 5236 Size: 5242 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3049 3431 4066 275 Size: 53 83 186 936 1516 382 635 1451 Fragments arranged by size: 1516 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2091 3675 4073 4347 4494 4905 2091 Size: 1584 398 274 147 411 2428 Fragments arranged by size: 2428 1584 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5017 Fragments arranged by size: 5017 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2153 1186 Size: 967 4275 BseYI C'CCAG_C Cuts at: 2508 4445 4941 2508 Size: 1937 496 2809 Fragments arranged by size: 2809 1937 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4669 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2692 3453 4481 1540 Size: 275 186 691 761 1028 2301 Fragments arranged by size: 2301 1028 761 691 275 186 BsiHKAI G_wGCw'C Cuts at: 3303 3388 4459 3303 Size: 85 1071 4086 Fragments arranged by size: 4086 1071 85 BsmI GAATG_Cn' Cuts at: 2753 2846 2753 Size: 93 5149 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 2225 2561 3303 3388 4459 1391 Size: 399 301 134 336 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 336 301 134 85 BspEI T'CCGG_A Cuts at: 4905 4905 Size: 5242 BspHI T'CATG_A Cuts at: 3131 4861 3131 Size: 1730 3512 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2597 844 Size: 1753 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2400 3129 1394 Size: 429 577 729 3507 Fragments arranged by size: 3507 729 577 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2310 3737 3911 65 Size: 1183 1062 1427 174 1396 Fragments arranged by size: 1427 1396 1183 1062 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3886 4073 1700 Size: 110 2076 187 2869 Fragments arranged by size: 2869 2076 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5242 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5242 BssSI C'ACGA_G Cuts at: 2010 3302 4314 2010 Size: 1292 1012 2938 Fragments arranged by size: 2938 1292 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5242 BstBI TT'CG_AA Cuts at: 4046 4046 Size: 5242 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5242 BstYI r'GATC_y Cuts at: 1972 2457 2572 2939 3324 3341 4793 4879 4891 Size: 485 115 367 385 17 1452 86 12 Cuts at: 4891 5237 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 485 385 367 346 115 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2106 2233 513 Size: 552 1041 127 3522 Fragments arranged by size: 3522 1041 552 127 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2505 487 Size: 683 56 1279 3224 Fragments arranged by size: 3224 1279 683 56 BtsI GCAGTG_nn' Cuts at: 2758 3552 3572 2758 Size: 794 20 4428 Fragments arranged by size: 4428 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5242 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5207 DraI TTT'AAA Cuts at: 2546 2546 Size: 5242 DraIII CAC_nnn'GTG Cuts at: 2284 2284 Size: 5242 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4249 809 Size: 507 2933 1802 Fragments arranged by size: 2933 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2689 3578 5207 Size: 54 1425 50 275 18 859 889 1629 Cuts at: 5207 8 Size: 43 Fragments arranged by size: 1629 1425 889 859 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2689 1537 Size: 275 877 4090 Fragments arranged by size: 4090 877 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3172 1353 Size: 1819 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2111 3829 4203 4349 387 Size: 1064 138 522 1718 374 146 1280 Fragments arranged by size: 1718 1280 1064 522 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2498 3305 3902 4689 4957 1239 Size: 393 524 342 807 597 787 268 1524 Fragments arranged by size: 1524 807 787 597 524 393 342 268 EcoO109I rG'GnC_Cy Cuts at: 2221 2222 2374 2221 Size: 1 152 5089 Fragments arranged by size: 5089 152 1 EcoRI G'AATT_C Cuts at: 2551 2929 2551 Size: 378 4864 FspI TGC'GCA Cuts at: 3748 3748 Size: 5242 HaeII r_GCGC'y Cuts at: 1678 4389 1678 Size: 2711 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3883 3915 3957 3989 1373 Size: 32 135 32 2311 32 42 32 2626 Fragments arranged by size: 2626 2311 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2586 2832 669 Size: 988 281 648 246 3079 Fragments arranged by size: 3079 988 648 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5242 HpaI GTT'AAC Cuts at: 2832 2832 Size: 5242 KpnI G_GTAC'C Cuts at: 2567 2567 Size: 5242 MfeI C'AATT_G Cuts at: 2841 2841 Size: 5242 MluI A'CGCG_T Cuts at: 5118 5118 Size: 5242 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2369 4356 4540 1305 Size: 1064 1987 184 2007 Fragments arranged by size: 2007 1987 1064 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3420 Fragments arranged by size: 3420 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3200 3559 3718 518 Size: 648 2034 359 159 2042 Fragments arranged by size: 2042 2034 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2476 2658 3335 4483 4728 1606 Size: 870 182 677 1148 245 2120 Fragments arranged by size: 2120 1148 870 677 245 182 NcoI C'CATG_G Cuts at: 513 1065 2233 513 Size: 552 1168 3522 Fragments arranged by size: 3522 1168 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5242 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5242 NotI GC'GGCC_GC Cuts at: 2689 2689 Size: 5242 NspI r_CATG'y Cuts at: 1132 1229 2193 2600 4145 1132 Size: 97 964 407 1545 2229 Fragments arranged by size: 2229 1545 964 407 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5242 PciI A'CATG_T Cuts at: 4141 4141 Size: 5242 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5242 PmeI GTTT'AAAC Cuts at: 2546 2546 Size: 5242 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3311 3343 4824 4856 3311 Size: 32 1481 32 3697 Fragments arranged by size: 3697 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2374 2374 Size: 5242 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5242 PsiI TTA'TAA Cuts at: 2812 2812 Size: 5242 PspOMI G'GGCC_C Cuts at: 2221 2221 Size: 5242 PstI C_TGCA'G Cuts at: 830 2230 2594 830 Size: 1400 364 3478 Fragments arranged by size: 3478 1400 364 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5242 SalI G'TCGA_C Cuts at: 1936 2584 1936 Size: 648 4594 SbfI CC_TGCA'GG Cuts at: 2230 2594 2230 Size: 364 4878 ScaI AGT'ACT Cuts at: 3490 5202 3490 Size: 1712 3530 SexAI A'CCwGG_T Cuts at: 2280 2280 Size: 5242 SfcI C'TryA_G Cuts at: 826 1045 2226 2590 3725 4406 4597 826 Size: 219 1181 364 1135 681 191 1471 Fragments arranged by size: 1471 1181 1135 681 364 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2229 2229 Size: 5242 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5242 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5242 SmaI CCC'GGG Cuts at: 1872 2081 2569 1872 Size: 209 488 4545 Fragments arranged by size: 4545 488 209 SmlI C'TyrA_G Cuts at: 820 1017 1943 2033 3346 4247 4509 820 Size: 197 926 90 1313 901 262 1553 Fragments arranged by size: 1553 1313 926 901 262 197 90 SnaBI TAC'GTA Cuts at: 493 3033 493 Size: 2540 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5242 SphI G_CATG'C Cuts at: 1132 2193 2600 1132 Size: 1061 407 3774 Fragments arranged by size: 3774 1061 407 SspI AAT'ATT Cuts at: 5 52 3166 5 Size: 47 3114 2081 Fragments arranged by size: 3114 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5242 StyI C'CwwG_G Cuts at: 513 1065 2233 2493 2536 2681 513 Size: 552 1168 260 43 145 3074 Fragments arranged by size: 3074 1168 552 260 145 43 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3269 3286 3439 3624 4069 325 Size: 1255 1689 17 153 185 445 1498 Fragments arranged by size: 1689 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3488 5200 96 Size: 274 80 33 51 1443 1511 1712 138 Fragments arranged by size: 1712 1511 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5202 5234 5202 Size: 32 5210 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5242 XbaI T'CTAG_A Cuts at: 2578 2578 Size: 5242 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5242 XmaI C'CCGG_G Cuts at: 1870 2079 2567 1870 Size: 209 488 4545 Fragments arranged by size: 4545 488 209 XmnI GAAnn'nnTTC Cuts at: 3371 3371 Size: 5242 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3050 276 Size: 53 83 186 936 1516 2468 Fragments arranged by size: 2468 1516 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfoI SrfI SwaI XcmI