Restriction map for
pFN21AE1222
##################################
# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI AspI AvaI
AviII AvrII BalI BbeI BbrPI
BbvCI BcgI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EarI
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FokI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI HphI KasI
Ksp632I KspI MaeIII MamI MfeI
MluI MluNI MroI MscI MseI
MspA1I MunI MvnI NaeI NarI
NgoMIV NheI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI SwaI TaqI Tru9I
Tsp45I Tsp509I Tth111I TthHB8I Van91I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 632 GGTACC G^GTACC
AhdI 567 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 282 GGGCCC GGGCC^C
Asp700I 408 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 632 GGTACC G^GTACC
AspEI 567 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 316 GAGCTC G[AT]GC[AT]^C
BamHI 109 GGATCC G^GATCC
BanI 632 GGTACC G^G[CT][GA]CC
BbsI 412 GTCTTC GAAGAC(2/6)
BfaI 307 CTAG C^TAG
BpuAI 412 GTCTTC GAAGAC(2/6)
BsaI 155 GAGACC GGTCTC(1/5)
BseRI 313 GAGGAG GAGGAG(10/8)
BsiEI 477 CGGCCG CG[GA][CT]^CG
BsiHKAI 316 GAGCTC G[AT]GC[AT]^C
Bsp1407I 680 TGTACA T^GTACA
BspMI 649 GCAGGT ACCTGC(4/8)
BsrGI 680 TGTACA T^GTACA
BsrI 629 ACTGG ACTGG(1/-1)
BstYI 109 GGATCC [GA]^GATC[CT]
DpnII 110 GATC ^GATC
EaeI 477 CGGCCG [CT]^GGCC[GA]
EagI 477 CGGCCG C^GGCCG
Eam1105I 567 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 477 CGGCCG C^GGCCG
Eco52I 477 CGGCCG C^GGCCG
EcoNI 564 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 646 CCCGC CCCGC(4/6)
HindIII 525 AAGCTT A^AGCTT
HinfI 498 GAATC G^A[ACGT]TC
KpnI 632 GGTACC GGTAC^C
MaeI 307 CTAG C^TAG
MaeII 71 ACGT A^CGT
MboI 110 GATC ^GATC
MflI 109 GGATCC [GA]^GATC[CT]
NcoI 176 CCATGG C^CATGG
NdeI 548 CATATG CA^TATG
NdeII 110 GATC ^GATC
NotI 476 GCGGCCGC GC^GGCCGC
NspI 460 GCATGT [GA]CATG^[CT]
SacI 316 GAGCTC GAGCT^C
Sau3AI 110 GATC ^GATC
SfaNI 445 GATGC GCATC(5/9)
SfiI 163 GGCCCTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 680 TGTACA T^GTACA
StuI 561 AGGCCT AGG^CCT
TfiI 498 GAATC G^A[AT]TC
XbaI 306 TCTAGA T^CTAGA
XhoII 109 GGATCC [GA]^GATC[CT]
XmnI 408 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 110 GATCC GGATC(4/5)
109 GGATC
AlwNI 41 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
705 CAGGAACTG
BbvI 40 GCAGC GCAGC(8/12)
222 GCTGC
BciVI 92 GTATCC GTATCC(6/5)
239 GTATCC
BcnI 97 CCCGG CC^[GC]GG
244 CCCGG
BglI 119 GCCTGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
164 GCCCTCCAGGC
BsmAI 155 GAGAC GTCTC(1/5)
587 GAGAC
DsaI 114 CCACGG C^C[GA][CT]GG
176 CCATGG
EcoT14I 176 CCATGG C^C[AT][AT]GG
493 CCAAGG
HapII 98 CCGG C^CGG
245 CCGG
HpaII 98 CCGG C^CGG
245 CCGG
MboII 534 GAAGA GAAGA(8/7)
413 TCTTC
MslI 30 CATCTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
304 CATCTAGATG
MspI 98 CCGG C^CGG
245 CCGG
NciI 97 CCCGG CC^[GC]GG
244 CCCGG
PpuMI 573 GGGTCCC [GA]G^G[AT]CC[CT]
607 GGGACCC
PstI 355 CTGCAG CTGCA^G
379 CTGCAG
SfcI 355 CTGCAG C^T[GA][CT]AG
379 CTGCAG
StyI 176 CCATGG C^C[AT][AT]GG
493 CCAAGG
TseI 40 GCAGC G^C[AT]GC
222 GCTGC
TspRI 667 CAGTG CA[GC]TG[ACGT][ACGT]^
694 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AfaI AluI AvaII BanII
BmyI BpmI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrDI BssKI BstNI
Cac8I Cfr13I DdeI DraII EcoO109I
EcoRII Fnu4HI HaeIII ItaI MnlI
MvaI MwoI NlaIII NlaIV RsaI
Sau96I ScrFI