(Circular) (Six-base) MAPSORT of: pFN21AE1222.seq Check: 8952 from: 1 to: 5440 pFN21AE1222 5440 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3250 278 Size: 53 83 186 936 1714 2468 Fragments arranged by size: 2468 1714 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2783 1937 Size: 846 4594 Acc65I G'GTAC_C Cuts at: 2637 2761 2637 Size: 124 5316 AclI AA'CG_TT Cuts at: 1563 3567 3940 1563 Size: 2004 373 3063 Fragments arranged by size: 3063 2004 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3503 4887 5155 1632 Size: 1871 1384 268 1917 Fragments arranged by size: 1917 1871 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5243 AflIII A'CryG_T Cuts at: 1203 4339 5316 1203 Size: 3136 977 1327 Fragments arranged by size: 3136 1327 977 AgeI A'CCGG_T Cuts at: 4271 4271 Size: 5440 AhdI GACnn_n'nnGTC Cuts at: 2577 4169 2577 Size: 1592 3848 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5440 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5440 AlwNI CAG_nnn'CTG Cuts at: 2051 2715 3183 4755 2051 Size: 664 468 1572 2736 Fragments arranged by size: 2736 1572 664 468 ApaI G_GGCC'C Cuts at: 2291 2291 Size: 5440 ApaLI G'TGCA_C Cuts at: 3497 4653 3497 Size: 1156 4284 ApoI r'AATT_y Cuts at: 1493 2749 2975 3127 5236 1493 Size: 1256 226 152 2109 1697 Fragments arranged by size: 2109 1697 1256 226 152 AseI AT'TA_AT Cuts at: 160 1032 3994 160 Size: 872 2962 1606 Fragments arranged by size: 2962 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5440 AvaI C'yCGr_G Cuts at: 1870 1943 2765 1870 Size: 73 822 4545 Fragments arranged by size: 4545 822 73 BamHI G'GATC_C Cuts at: 2114 2770 3137 2114 Size: 656 367 4417 Fragments arranged by size: 4417 656 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2637 2761 4216 618 Size: 325 217 627 850 124 1455 1842 Fragments arranged by size: 1842 1455 850 627 325 217 124 BanII G_rGCy'C Cuts at: 1391 2239 2291 2325 2377 2393 2652 2759 1391 Size: 848 52 34 52 16 259 107 4072 Fragments arranged by size: 4072 848 259 107 52 52 34 16 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2410 928 Size: 971 511 3958 Fragments arranged by size: 3958 971 511 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3631 3665 1903 Size: 34 1694 34 3678 Fragments arranged by size: 3678 1694 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2108 2255 3332 4542 2108 Size: 147 1077 1210 3006 Fragments arranged by size: 3006 1210 1077 147 BclI T'GATC_A Cuts at: 1548 1548 Size: 5440 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2130 2175 4051 Size: 107 122 71 631 605 458 45 1876 Cuts at: 4051 136 Size: 1525 Fragments arranged by size: 1876 1525 631 605 458 122 107 71 45 BglII A'GATC_T Cuts at: 5435 5435 Size: 5440 BlpI GC'TnA_GC Cuts at: 2857 2857 Size: 5440 Bme1580I G_kGCm'C Cuts at: 1790 2018 2291 3501 4657 1790 Size: 228 273 1210 1156 2573 Fragments arranged by size: 2573 1210 1156 273 228 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5440 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3171 4129 442 Size: 416 53 83 399 24 1754 958 1753 Fragments arranged by size: 1754 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5440 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2307 2307 Size: 5440 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2157 2214 2253 2277 2407 2610 4100 1239 Size: 918 57 39 24 130 203 1490 2579 Fragments arranged by size: 2579 1490 918 203 130 57 39 24 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3565 4430 4728 3565 Size: 865 298 4277 Fragments arranged by size: 4277 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2154 4103 882 Size: 617 655 1949 2219 Fragments arranged by size: 2219 1949 655 617 BsaAI yAC'GTr Cuts at: 493 1204 3231 493 Size: 711 2027 2702 Fragments arranged by size: 2702 2027 711 BsaBI GATnn'nnATC Cuts at: 5434 5434 Size: 5440 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3247 3629 4264 275 Size: 53 83 186 936 1714 382 635 1451 Fragments arranged by size: 1714 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3873 4271 4545 4692 5103 3873 Size: 398 274 147 411 4210 Fragments arranged by size: 4210 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2134 2164 2400 2430 1178 Size: 30 165 30 731 30 236 30 4188 Fragments arranged by size: 4188 731 236 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2333 1186 Size: 1147 4293 BseYI C'CCAG_C Cuts at: 2582 4643 5139 2582 Size: 2061 496 2883 Fragments arranged by size: 2883 2061 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4867 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2485 2890 3651 4679 1540 Size: 275 186 484 405 761 1028 2301 Fragments arranged by size: 2301 1028 761 484 405 275 186 BsiHKAI G_wGCw'C Cuts at: 2325 3501 3586 4657 2325 Size: 1176 85 1071 3108 Fragments arranged by size: 3108 1176 1071 85 BsmI GAATG_Cn' Cuts at: 2951 3044 2951 Size: 93 5347 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2239 2291 2325 2377 2393 2652 Size: 399 228 221 52 34 52 16 259 Cuts at: 2652 2759 3501 3586 4657 1391 Size: 107 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 259 228 221 107 85 52 52 34 16 BspEI T'CCGG_A Cuts at: 5103 5103 Size: 5440 BspHI T'CATG_A Cuts at: 3329 5059 3329 Size: 1730 3710 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2645 2795 844 Size: 1801 150 3489 Fragments arranged by size: 3489 1801 150 BsrBI CCG'CTC Cuts at: 1394 1823 3327 1394 Size: 429 1504 3507 Fragments arranged by size: 3507 1504 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2434 2487 2523 3935 4109 65 Size: 1183 1186 53 36 1412 174 1396 Fragments arranged by size: 1412 1396 1186 1183 174 53 36 BsrFI r'CCGG_y Cuts at: 1700 1810 4084 4271 1700 Size: 110 2274 187 2869 Fragments arranged by size: 2869 2274 187 110 BsrGI T'GTAC_A Cuts at: 96 2685 96 Size: 2589 2851 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5440 BssSI C'ACGA_G Cuts at: 3500 4512 3500 Size: 1012 4428 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5440 BstBI TT'CG_AA Cuts at: 4244 4244 Size: 5440 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5440 BstYI r'GATC_y Cuts at: 1972 2114 2770 3137 3522 3539 4991 5077 5089 Size: 142 656 367 385 17 1452 86 12 Cuts at: 5089 5435 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 656 385 367 346 142 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2119 2181 513 Size: 552 1054 62 3772 Fragments arranged by size: 3772 1054 552 62 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4701 Fragments arranged by size: 4701 683 56 BtsI GCAGTG_nn' Cuts at: 2678 2705 2956 3750 3770 2678 Size: 27 251 794 20 4348 Fragments arranged by size: 4348 794 251 27 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5440 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5405 DraI TTT'AAA Cuts at: 2744 2744 Size: 5440 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4447 809 Size: 507 3131 1802 Fragments arranged by size: 3131 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2482 2887 3776 Size: 54 1425 50 275 18 652 405 889 Cuts at: 3776 5405 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 652 405 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2482 2887 1537 Size: 275 670 405 4090 Fragments arranged by size: 4090 670 405 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3370 1353 Size: 2017 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4027 4401 4547 387 Size: 1064 138 2438 374 146 1280 Fragments arranged by size: 2438 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2323 2323 Size: 5440 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2157 2214 2253 2277 2407 2610 3503 Size: 393 525 57 39 24 130 203 893 Cuts at: 3503 4100 4887 5155 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 893 787 597 525 393 268 203 130 57 39 24 EcoNI CCTnn'n_nnAGG Cuts at: 2573 2573 Size: 5440 EcoO109I rG'GnC_Cy Cuts at: 2177 2287 2288 2579 2613 2177 Size: 110 1 291 34 5004 Fragments arranged by size: 5004 291 110 34 1 EcoRI G'AATT_C Cuts at: 2749 3127 2749 Size: 378 5062 FspI TGC'GCA Cuts at: 3946 3946 Size: 5440 HaeII r_GCGC'y Cuts at: 1678 4587 1678 Size: 2909 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2136 2168 2340 2372 2400 Size: 32 135 32 564 32 172 32 28 Cuts at: 2400 2432 2563 2595 2721 2753 4081 4113 4155 Size: 32 131 32 126 32 1328 32 42 Cuts at: 4155 4187 1373 Size: 32 2626 Fragments arranged by size: 2626 1328 564 172 135 131 126 42 32 32 32 32 32 32 32 32 32 28 HincII GTy'rAC Cuts at: 669 1657 1938 2784 3030 669 Size: 988 281 846 246 3079 Fragments arranged by size: 3079 988 846 281 246 HindIII A'AGCT_T Cuts at: 748 2530 748 Size: 1782 3658 HpaI GTT'AAC Cuts at: 3030 3030 Size: 5440 KpnI G_GTAC'C Cuts at: 2641 2765 2641 Size: 124 5316 MfeI C'AATT_G Cuts at: 3039 3039 Size: 5440 MluI A'CGCG_T Cuts at: 5316 5316 Size: 5440 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2065 4554 4738 1305 Size: 760 2489 184 2007 Fragments arranged by size: 2489 2007 760 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3618 Fragments arranged by size: 3618 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2039 2313 3398 3757 3916 518 Size: 648 873 274 1085 359 159 2042 Fragments arranged by size: 2042 1085 873 648 359 274 159 MspA1I CmG'CkG Cuts at: 1606 2856 3533 4681 4926 1606 Size: 1250 677 1148 245 2120 Fragments arranged by size: 2120 1250 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2181 513 Size: 552 1116 3772 Fragments arranged by size: 3772 1116 552 NdeI CA'TA_TG Cuts at: 387 2554 387 Size: 2167 3273 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5440 NotI GC'GGCC_GC Cuts at: 2482 2887 2482 Size: 405 5035 NspI r_CATG'y Cuts at: 1132 1229 2469 2798 4343 1132 Size: 97 1240 329 1545 2229 Fragments arranged by size: 2229 1545 1240 329 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5440 PciI A'CATG_T Cuts at: 4339 4339 Size: 5440 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5440 PfoI T'CCnGG_A Cuts at: 2110 2212 2110 Size: 102 5338 PmeI GTTT'AAAC Cuts at: 2744 2744 Size: 5440 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3509 3541 5022 5054 3509 Size: 32 1481 32 3895 Fragments arranged by size: 3895 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2579 2613 2579 Size: 34 5406 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5440 PsiI TTA'TAA Cuts at: 3010 3010 Size: 5440 PspOMI G'GGCC_C Cuts at: 2287 2287 Size: 5440 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2705 2737 2705 Size: 32 5408 PstI C_TGCA'G Cuts at: 830 2364 2388 2792 830 Size: 1534 24 404 3478 Fragments arranged by size: 3478 1534 404 24 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5440 SacI G_AGCT'C Cuts at: 2325 2325 Size: 5440 SalI G'TCGA_C Cuts at: 1936 2782 1936 Size: 846 4594 SanDI GG'GwC_CC Cuts at: 2579 2613 2579 Size: 34 5406 SbfI CC_TGCA'GG Cuts at: 2792 2792 Size: 5440 ScaI AGT'ACT Cuts at: 3688 5400 3688 Size: 1712 3728 SfcI C'TryA_G Cuts at: 826 1045 2360 2384 2788 3923 4604 4795 826 Size: 219 1315 24 404 1135 681 191 1471 Fragments arranged by size: 1471 1315 1135 681 404 219 191 24 SfiI GGCCn_nnn'nGGCC Cuts at: 2175 2175 Size: 5440 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5440 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5440 SmaI CCC'GGG Cuts at: 1872 2767 1872 Size: 895 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3544 4445 4707 820 Size: 197 926 1601 901 262 1553 Fragments arranged by size: 1601 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3231 493 Size: 2738 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5440 SphI G_CATG'C Cuts at: 1132 2798 1132 Size: 1666 3774 SspI AAT'ATT Cuts at: 5 52 3364 5 Size: 47 3312 2081 Fragments arranged by size: 3312 2081 47 StuI AGG'CCT Cuts at: 1517 2568 1517 Size: 1051 4389 StyI C'CwwG_G Cuts at: 513 1065 2181 2498 2879 513 Size: 552 1116 317 381 3074 Fragments arranged by size: 3074 1116 552 381 317 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3467 3484 3637 3822 4267 325 Size: 1255 1887 17 153 185 445 1498 Fragments arranged by size: 1887 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2544 2685 2721 Size: 274 80 33 51 1443 567 141 36 Cuts at: 2721 3686 5398 96 Size: 965 1712 138 Fragments arranged by size: 1712 1443 965 567 274 141 138 80 51 36 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2134 2166 5400 5432 2134 Size: 32 3234 32 2142 Fragments arranged by size: 3234 2142 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5440 XbaI T'CTAG_A Cuts at: 2311 2776 2311 Size: 465 4975 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5440 XmaI C'CCGG_G Cuts at: 1870 2765 1870 Size: 895 4545 XmnI GAAnn'nnTTC Cuts at: 2417 3569 2417 Size: 1152 4288 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3248 276 Size: 53 83 186 936 1714 2468 Fragments arranged by size: 2468 1714 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PvuII RsrII SacII SapI SexAI SfoI SrfI SwaI XcmI