(Linear) (Six-base) MAPSORT of: KIEE1222.seq Check: 4487 from: 1 to: 735 KIEE1222.orf With 182 enzymes: SgfI * July 15, 2010 18:35 .. Acc65I G'GTAC_C Cuts at: 0 632 735 Size: 632 103 AhdI GACnn_n'nnGTC Cuts at: 0 572 735 Size: 572 163 AlwNI CAG_nnn'CTG Cuts at: 0 46 710 735 Size: 46 664 25 Fragments arranged by size: 664 46 25 ApaI G_GGCC'C Cuts at: 0 286 735 Size: 286 449 BamHI G'GATC_C Cuts at: 0 109 735 Size: 109 626 BanI G'GyrC_C Cuts at: 0 632 735 Size: 632 103 BanII G_rGCy'C Cuts at: 0 234 286 320 372 388 647 735 Size: 234 52 34 52 16 259 88 Fragments arranged by size: 259 234 88 52 52 34 16 BbsI GAAGACnn'nnnn_ Cuts at: 0 405 735 Size: 405 330 BciVI GTATCCnnnnn_n' Cuts at: 0 103 250 735 Size: 103 147 485 Fragments arranged by size: 485 147 103 BglI GCCn_nnn'nGGC Cuts at: 0 125 170 735 Size: 125 45 565 Fragments arranged by size: 565 125 45 Bme1580I G_kGCm'C Cuts at: 0 13 286 735 Size: 13 273 449 Fragments arranged by size: 449 273 13 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 302 735 Size: 302 433 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 152 209 248 272 402 605 735 Size: 152 57 39 24 130 203 130 Fragments arranged by size: 203 152 130 130 57 39 24 BsaI GGTCTCn'nnnn_ Cuts at: 0 149 735 Size: 149 586 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 129 159 395 425 735 Size: 129 30 236 30 310 Fragments arranged by size: 310 236 129 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 328 735 Size: 328 407 BseYI C'CCAG_C Cuts at: 0 577 735 Size: 577 158 BsiEI CG_ry'CG Cuts at: 0 480 735 Size: 480 255 BsiHKAI G_wGCw'C Cuts at: 0 320 735 Size: 320 415 Bsp1286I G_dGCh'C Cuts at: 0 13 234 286 320 372 388 647 735 Size: 13 221 52 34 52 16 259 88 Fragments arranged by size: 259 221 88 52 52 34 16 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 640 735 Size: 640 95 BsrDI GCAATG_nn' Cuts at: 0 429 482 518 735 Size: 429 53 36 217 Fragments arranged by size: 429 217 53 36 BsrGI T'GTAC_A Cuts at: 0 680 735 Size: 680 55 BstYI r'GATC_y Cuts at: 0 109 735 Size: 109 626 BtgI C'CryG_G Cuts at: 0 114 176 735 Size: 114 62 559 Fragments arranged by size: 559 114 62 BtsI GCAGTG_nn' Cuts at: 0 673 700 735 Size: 673 27 35 Fragments arranged by size: 673 35 27 EaeI y'GGCC_r Cuts at: 0 477 735 Size: 477 258 EagI C'GGCC_G Cuts at: 0 477 735 Size: 477 258 EcoICRI GAG'CTC Cuts at: 0 318 735 Size: 318 417 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 152 209 248 272 402 605 735 Size: 152 57 39 24 130 203 130 Fragments arranged by size: 203 152 130 130 57 39 24 EcoNI CCTnn'n_nnAGG Cuts at: 0 568 735 Size: 568 167 EcoO109I rG'GnC_Cy Cuts at: 0 172 282 283 574 608 735 Size: 172 110 1 291 34 127 Fragments arranged by size: 291 172 127 110 34 1 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 131 163 335 367 395 427 558 590 Size: 131 32 172 32 28 32 131 32 Cuts at: 590 716 735 Size: 126 19 Fragments arranged by size: 172 131 131 126 32 32 32 32 28 19 HindIII A'AGCT_T Cuts at: 0 525 735 Size: 525 210 KpnI G_GTAC'C Cuts at: 0 636 735 Size: 636 99 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 60 735 Size: 60 675 MslI CAynn'nnrTG Cuts at: 0 34 308 735 Size: 34 274 427 Fragments arranged by size: 427 274 34 NcoI C'CATG_G Cuts at: 0 176 735 Size: 176 559 NdeI CA'TA_TG Cuts at: 0 549 735 Size: 549 186 NotI GC'GGCC_GC Cuts at: 0 477 735 Size: 477 258 NspI r_CATG'y Cuts at: 0 464 735 Size: 464 271 PfoI T'CCnGG_A Cuts at: 0 105 207 735 Size: 105 102 528 Fragments arranged by size: 528 105 102 PpuMI rG'GwC_Cy Cuts at: 0 574 608 735 Size: 574 34 127 Fragments arranged by size: 574 127 34 PspOMI G'GGCC_C Cuts at: 0 282 735 Size: 282 453 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 700 732 735 Size: 700 32 3 Fragments arranged by size: 700 32 3 PstI C_TGCA'G Cuts at: 0 359 383 735 Size: 359 24 352 Fragments arranged by size: 359 352 24 SacI G_AGCT'C Cuts at: 0 320 735 Size: 320 415 SanDI GG'GwC_CC Cuts at: 0 574 608 735 Size: 574 34 127 Fragments arranged by size: 574 127 34 SfcI C'TryA_G Cuts at: 0 355 379 735 Size: 355 24 356 Fragments arranged by size: 356 355 24 SfiI GGCCn_nnn'nGGCC Cuts at: 0 170 735 Size: 170 565 StuI AGG'CCT Cuts at: 0 563 735 Size: 563 172 StyI C'CwwG_G Cuts at: 0 176 493 735 Size: 176 317 242 Fragments arranged by size: 317 242 176 TatI w'GTAC_w Cuts at: 0 539 680 716 735 Size: 539 141 36 19 Fragments arranged by size: 539 141 36 19 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 129 161 735 Size: 129 32 574 Fragments arranged by size: 574 129 32 XbaI T'CTAG_A Cuts at: 0 306 735 Size: 306 429 XmnI GAAnn'nnTTC Cuts at: 0 412 735 Size: 412 323 Enzymes that do cut: Acc65I AhdI AlwNI ApaI BamHI BanI BanII BbsI BciVI BglI Bme1580I BplI BpmI BsaI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrDI BsrGI BstYI BtgI BtsI EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I Hin4I HindIII KpnI MmeI MslI NcoI NdeI NotI NspI PfoI PpuMI PspOMI PsrI PstI SacI SanDI SfcI SfiI StuI StyI TatI TstI XbaI XmnI Enzymes that do not cut: AarI AatII AccI AclI AcuI AfeI AflII AflIII AgeI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI BclI BfrBI BglII BlpI BmgBI BmrI BmtI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsgI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EarI EciI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HpaI KasI MfeI MluI MscI MspA1I NaeI NarI NgoMIV NheI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I XcmI XhoI XmaI ZraI