################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AccI AccII AccIII AclI AcsI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AvrII BamHI BbeI BcgI BclI BfaI BfrI BglII BlnI BsaBI BsaWI BseAI BsiEI BsiHKAI BsiWI BspDI BspEI BspLU11I BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I ClaI CpoI DraI DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HaeII HincII HindII HindIII HpaI KasI Ksp632I KspI MaeI MamI MfeI MflI MluI MroI MseI MslI MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SspI SwaI TfiI Tru9I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 485 GGTACC G^GTACC AflIII 641 ACGTGT A^C[GA][CT]GT AlwNI 124 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 485 GGTACC G^GTACC AvaI 1087 CCCGAG C^[CT]CG[GA]G AviII 724 TGCGCA TGC^GCA BanI 485 GGTACC G^G[CT][GA]CC BbrPI 640 CACGTG CAC^GTG BbsI 1045 GTCTTC GAAGAC(2/6) BciVI 960 GTATCC GTATCC(6/5) BglI 407 GCCTGATCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 976 GCTGAGC GC^T[ACGT]AGC Bpu1102I 976 GCTGAGC GC^T[ACGT]AGC BpuAI 1045 GTCTTC GAAGAC(2/6) BsaAI 640 CACGTG [CT]AC^GT[GA] BsaI 340 GAGACC GGTCTC(1/5) BsmBI 951 CGTCTC CGTCTC(1/5) BsmI 371 GCATTC GAATGC(1/-1) BsoBI 1087 CCCGAG C^[CT]CG[GA]G Bsp1407I 248 TGTACA T^GTACA BspHI 77 TCATGA T^CATGA BspMI 1034 ACCTGC ACCTGC(4/8) BsrBI 6 GAGCGG CCGCTC(-3/-3) BsrGI 248 TGTACA T^GTACA CelII 976 GCTGAGC GC^T[ACGT]AGC DrdI 85 GACTCCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 893 CCGTGG C^C[GA][CT]GG EcoNI 1074 CCTCACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 724 TGCGCA TGC^GCA HinfI 85 GACTC G^A[ACGT]TC KpnI 485 GGTACC GGTAC^C PleI 85 GACTC GAGTC(4/5) PmaCI 640 CACGTG CAC^GTG PmlI 640 CACGTG CAC^GTG RcaI 77 TCATGA T^CATGA SbfI 1127 CCTGCAGG CCTGCA^GG SphI 479 GCATGC GCATG^C Sse8387I 1127 CCTGCAGG CCTGCA^GG SspBI 248 TGTACA T^GTACA StuI 380 AGGCCT AGG^CCT Tsp509I 808 AATT ^AATT ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AatII 751 GACGTC GACGT^C 949 GACGTC AlwI 74 GGATC GGATC(4/5) 861 GGATC AocI 756 CCTGAGG CC^T[ACGT]AGG 934 CCTGAGG AspI 307 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 748 GACGACGTC BalI 401 TGGCCA TGG^CCA 650 TGGCCA BbvCI 210 GCTGAGG CCTCAGC(-5/-2) 1038 GCTGAGG BpmI 295 CTGGAG CTGGAG(16/14) 943 CTGGAG BsgI 39 CTGCAC GTGCAG(16/14) 819 CTGCAC Bsu36I 756 CCTGAGG CC^T[ACGT]AGG 934 CCTGAGG Eco81I 756 CCTGAGG CC^T[ACGT]AGG 934 CCTGAGG FauI 1104 CCCGC CCCGC(4/6) 459 GCGGG MaeIII 355 GTCAC ^GT[ACGT]AC 576 GTCAC MboII 695 TCTTC GAAGA(8/7) 1046 TCTTC MluNI 401 TGGCCA TGG^CCA 650 TGGCCA MscI 401 TGGCCA TGG^CCA 650 TGGCCA NspI 320 GCATGT [GA]CATG^[CT] 479 GCATGC PflFI 307 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 748 GACGACGTC TaqI 629 TCGA T^CGA 1049 TCGA Tsp45I 355 GTCAC ^GT[GC]AC 576 GTCAC Tth111I 307 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 748 GACGACGTC TthHB8I 629 TCGA T^CGA 1049 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AcyI AfaI AluI AvaII BanII BbvI BcnI BmyI BsaHI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI MaeII MboI MnlI MspA1I MspI MvaI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfcI StyI TseI TspRI