(Circular) (Six-base) MAPSORT of: pFN21AE1306.seq Check: 2024 from: 1 to: 5938 pFN21AE1306 5938 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2760 2958 3748 278 Size: 53 83 186 936 1224 198 790 2468 Fragments arranged by size: 2468 1224 936 790 198 186 83 53 AccI GT'mk_AC Cuts at: 1937 3281 1937 Size: 1344 4594 Acc65I G'GTAC_C Cuts at: 2490 3259 2490 Size: 769 5169 AclI AA'CG_TT Cuts at: 1563 4065 4438 1563 Size: 2502 373 3063 Fragments arranged by size: 3063 2502 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2686 4001 5385 5653 1632 Size: 1054 1315 1384 268 1917 Fragments arranged by size: 1917 1384 1315 1054 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5741 AflIII A'CryG_T Cuts at: 1203 2646 4837 5814 1203 Size: 1443 2191 977 1327 Fragments arranged by size: 2191 1443 1327 977 AgeI A'CCGG_T Cuts at: 4769 4769 Size: 5938 AhdI GACnn_n'nnGTC Cuts at: 4667 4667 Size: 5938 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5938 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5938 AlwNI CAG_nnn'CTG Cuts at: 2134 3681 5253 2134 Size: 1547 1572 2819 Fragments arranged by size: 2819 1572 1547 ApaLI G'TGCA_C Cuts at: 3995 5151 3995 Size: 1156 4782 ApoI r'AATT_y Cuts at: 1493 3247 3473 3625 5734 1493 Size: 1754 226 152 2109 1697 Fragments arranged by size: 2109 1754 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4492 160 Size: 872 3460 1606 Fragments arranged by size: 3460 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5938 AvaI C'yCGr_G Cuts at: 1870 1943 3092 3263 1870 Size: 73 1149 171 4545 Fragments arranged by size: 4545 1149 171 73 BamHI G'GATC_C Cuts at: 3268 3635 3268 Size: 367 5571 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2490 3259 4714 618 Size: 325 217 627 703 769 1455 1842 Fragments arranged by size: 1842 1455 769 703 627 325 217 BanII G_rGCy'C Cuts at: 1391 2565 2692 3105 3192 3257 1391 Size: 1174 127 413 87 65 4072 Fragments arranged by size: 4072 1174 413 127 87 65 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3043 928 Size: 971 1144 3823 Fragments arranged by size: 3823 1144 971 BbvCI CC'TCA_GC Cuts at: 2216 3044 2216 Size: 828 5110 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4129 4163 1903 Size: 34 2192 34 3678 Fragments arranged by size: 3678 2192 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2976 3830 5040 2976 Size: 854 1210 3874 Fragments arranged by size: 3874 1210 854 BclI T'GATC_A Cuts at: 1548 1548 Size: 5938 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2418 4549 136 Size: 107 122 71 631 605 746 2131 1525 Fragments arranged by size: 2131 1525 746 631 605 122 107 71 BglII A'GATC_T Cuts at: 5933 5933 Size: 5938 BlpI GC'TnA_GC Cuts at: 2982 3355 2982 Size: 373 5565 Bme1580I G_kGCm'C Cuts at: 1790 2112 2470 3999 5155 1790 Size: 322 358 1529 1156 2573 Fragments arranged by size: 2573 1529 1156 358 322 BmgBI CAC'GTC Cuts at: 1318 2314 1318 Size: 996 4942 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3193 3669 4627 Size: 416 53 83 399 24 1776 476 958 Cuts at: 4627 442 Size: 1753 Fragments arranged by size: 1776 1753 958 476 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5938 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2974 2974 Size: 5938 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2321 2969 4598 1239 Size: 1082 648 1629 2579 Fragments arranged by size: 2579 1629 1082 648 Bpu10I CC'TnA_GC Cuts at: 2216 2990 3044 2216 Size: 774 54 5110 Fragments arranged by size: 5110 774 54 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4063 4928 5226 4063 Size: 865 298 4775 Fragments arranged by size: 4775 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2339 4601 882 Size: 617 840 2262 2219 Fragments arranged by size: 2262 2219 840 617 BsaAI yAC'GTr Cuts at: 493 1204 2647 3729 493 Size: 711 1443 1082 2702 Fragments arranged by size: 2702 1443 1082 711 BsaBI GATnn'nnATC Cuts at: 5932 5932 Size: 5938 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2283 2457 2757 2955 Size: 53 83 186 936 750 174 300 198 Cuts at: 2955 3745 4127 4762 275 Size: 790 382 635 1451 Fragments arranged by size: 1451 936 790 750 635 382 300 198 186 174 83 53 BsaWI w'CCGG_w Cuts at: 4371 4769 5043 5190 5601 4371 Size: 398 274 147 411 4708 Fragments arranged by size: 4708 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3073 3103 3137 3167 1178 Size: 30 165 30 1670 30 34 30 3949 Fragments arranged by size: 3949 1670 165 34 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2701 3110 3113 3145 1186 Size: 1515 409 3 32 3979 Fragments arranged by size: 3979 1515 409 32 3 BseYI C'CCAG_C Cuts at: 2479 3121 5141 5637 2479 Size: 642 2020 496 2780 Fragments arranged by size: 2780 2020 642 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2029 2809 1163 Size: 573 293 780 4292 Fragments arranged by size: 4292 780 573 293 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3388 4149 5177 1540 Size: 275 186 1387 761 1028 2301 Fragments arranged by size: 2301 1387 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3999 4084 5155 3999 Size: 85 1071 4782 Fragments arranged by size: 4782 1071 85 BsmI GAATG_Cn' Cuts at: 2376 3449 3542 2376 Size: 1073 93 4772 Fragments arranged by size: 4772 1073 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2962 2962 Size: 5938 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2112 2470 2565 2692 3105 3192 3257 Size: 399 322 358 95 127 413 87 65 Cuts at: 3257 3999 4084 5155 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 413 399 358 322 127 95 87 85 65 BspEI T'CCGG_A Cuts at: 5601 5601 Size: 5938 BspHI T'CATG_A Cuts at: 2082 3827 5557 2082 Size: 1745 1730 2463 Fragments arranged by size: 2463 1745 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3048 3293 844 Size: 2204 245 3489 Fragments arranged by size: 3489 2204 245 BsrBI CCG'CTC Cuts at: 1394 1823 2013 3825 1394 Size: 429 190 1812 3507 Fragments arranged by size: 3507 1812 429 190 BsrDI GCAATG_nn' Cuts at: 65 1248 4433 4607 65 Size: 1183 3185 174 1396 Fragments arranged by size: 3185 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2151 2189 2222 2372 2736 4582 4769 Size: 110 341 38 33 150 364 1846 187 Cuts at: 4769 1700 Size: 2869 Fragments arranged by size: 2869 1846 364 341 187 150 110 38 33 BsrGI T'GTAC_A Cuts at: 96 2253 96 Size: 2157 3781 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5938 BssSI C'ACGA_G Cuts at: 3998 5010 3998 Size: 1012 4926 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5938 BstBI TT'CG_AA Cuts at: 4742 4742 Size: 5938 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5938 BstXI CCAn_nnnn'nTGG Cuts at: 2899 3112 3125 2899 Size: 213 13 5712 Fragments arranged by size: 5712 213 13 BstYI r'GATC_y Cuts at: 1972 3268 3635 4020 4037 5489 5575 5587 5933 Size: 1296 367 385 17 1452 86 12 346 Cuts at: 5933 1972 Size: 1977 Fragments arranged by size: 1977 1452 1296 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2762 2940 2762 Size: 178 5760 BtgI C'CryG_G Cuts at: 513 1065 2898 513 Size: 552 1833 3553 Fragments arranged by size: 3553 1833 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3161 487 Size: 683 56 1935 3264 Fragments arranged by size: 3264 1935 683 56 BtsI GCAGTG_nn' Cuts at: 2046 2374 3454 4248 4268 2046 Size: 328 1080 794 20 3716 Fragments arranged by size: 3716 1080 794 328 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5938 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5903 DraI TTT'AAA Cuts at: 3242 3242 Size: 5938 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2096 4945 809 Size: 507 780 2849 1802 Fragments arranged by size: 2849 1802 780 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2187 2406 Size: 54 1425 50 275 18 207 150 219 Cuts at: 2406 2431 2655 2853 3385 4274 5903 8 Size: 25 224 198 532 889 1629 43 Fragments arranged by size: 1629 1425 889 532 275 224 219 207 198 150 54 50 43 25 18 EagI C'GGCC_G Cuts at: 1537 1812 3385 1537 Size: 275 1573 4090 Fragments arranged by size: 4090 1573 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3868 1353 Size: 2515 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3026 4525 4899 5045 387 Size: 1064 138 1437 1499 374 146 1280 Fragments arranged by size: 1499 1437 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2321 2686 2969 4001 4598 5385 5653 Size: 393 689 365 283 1032 597 787 268 Cuts at: 5653 1239 Size: 1524 Fragments arranged by size: 1524 1032 787 689 597 393 365 283 268 EcoNI CCTnn'n_nnAGG Cuts at: 3083 3083 Size: 5938 EcoO109I rG'GnC_Cy Cuts at: 2626 2935 3138 2626 Size: 309 203 5426 Fragments arranged by size: 5426 309 203 EcoRI G'AATT_C Cuts at: 3247 3625 3247 Size: 378 5560 FspI TGC'GCA Cuts at: 2731 4444 2731 Size: 1713 4225 HaeII r_GCGC'y Cuts at: 1678 5085 1678 Size: 3407 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2679 2711 4579 4611 4653 Size: 32 135 32 1107 32 1868 32 42 Cuts at: 4653 4685 1373 Size: 32 2626 Fragments arranged by size: 2626 1868 1107 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3282 3528 669 Size: 988 281 1344 246 3079 Fragments arranged by size: 3079 1344 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5938 HpaI GTT'AAC Cuts at: 3528 3528 Size: 5938 KpnI G_GTAC'C Cuts at: 2494 3263 2494 Size: 769 5169 MfeI C'AATT_G Cuts at: 3537 3537 Size: 5938 MluI A'CGCG_T Cuts at: 5814 5814 Size: 5938 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5052 5236 1305 Size: 3747 184 2007 Fragments arranged by size: 3747 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2408 2657 10 Size: 54 1425 343 576 249 3291 Fragments arranged by size: 3291 1425 576 343 249 54 MslI CAynn'nnrTG Cuts at: 518 1166 3896 4255 4414 518 Size: 648 2730 359 159 2042 Fragments arranged by size: 2730 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2131 2479 2511 2680 3112 3121 3354 4031 Size: 525 348 32 169 432 9 233 677 Cuts at: 4031 5179 5424 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 525 432 348 245 233 169 32 9 NaeI GCC'GGC Cuts at: 2191 2224 2374 2738 2191 Size: 33 150 364 5391 Fragments arranged by size: 5391 364 150 33 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5386 NdeI CA'TA_TG Cuts at: 387 387 Size: 5938 NgoMIV G'CCGG_C Cuts at: 2189 2222 2372 2736 2189 Size: 33 150 364 5391 Fragments arranged by size: 5391 364 150 33 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5938 NotI GC'GGCC_GC Cuts at: 3385 3385 Size: 5938 NspI r_CATG'y Cuts at: 1132 1229 2329 2488 3296 4841 1132 Size: 97 1100 159 808 1545 2229 Fragments arranged by size: 2229 1545 1100 808 159 97 PasI CC'CwG_GG Cuts at: 1348 2523 1348 Size: 1175 4763 PciI A'CATG_T Cuts at: 4837 4837 Size: 5938 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5938 PfoI T'CCnGG_A Cuts at: 2391 2391 Size: 5938 PmeI GTTT'AAAC Cuts at: 3242 3242 Size: 5938 PmlI CAC'GTG Cuts at: 2647 2647 Size: 5938 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4007 4039 5520 5552 4007 Size: 32 1481 32 4393 Fragments arranged by size: 4393 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2626 2935 3138 2626 Size: 309 203 5426 Fragments arranged by size: 5426 309 203 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5938 PsiI TTA'TAA Cuts at: 3508 3508 Size: 5938 PstI C_TGCA'G Cuts at: 830 2053 2160 2679 3137 3290 830 Size: 1223 107 519 458 153 3478 Fragments arranged by size: 3478 1223 519 458 153 107 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5938 PvuII CAG'CTG Cuts at: 2131 2479 2511 2680 3121 2131 Size: 348 32 169 441 4948 Fragments arranged by size: 4948 441 348 169 32 SalI G'TCGA_C Cuts at: 1936 3280 1936 Size: 1344 4594 SanDI GG'GwC_CC Cuts at: 2935 2935 Size: 5938 SbfI CC_TGCA'GG Cuts at: 3137 3290 3137 Size: 153 5785 ScaI AGT'ACT Cuts at: 4186 5898 4186 Size: 1712 4226 SfcI C'TryA_G Cuts at: 826 1045 2049 2156 2675 2719 3133 3286 4421 Size: 219 1004 107 519 44 414 153 1135 Cuts at: 4421 5102 5293 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1004 681 519 414 219 191 153 107 44 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5938 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5938 SmaI CCC'GGG Cuts at: 1872 3265 1872 Size: 1393 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4042 4943 5205 820 Size: 197 926 2099 901 262 1553 Fragments arranged by size: 2099 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3729 493 Size: 3236 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5938 SphI G_CATG'C Cuts at: 1132 2488 3296 1132 Size: 1356 808 3774 Fragments arranged by size: 3774 1356 808 SspI AAT'ATT Cuts at: 5 52 3862 5 Size: 47 3810 2081 Fragments arranged by size: 3810 2081 47 StuI AGG'CCT Cuts at: 1517 2387 1517 Size: 870 5068 StyI C'CwwG_G Cuts at: 513 1065 2184 2572 2930 3377 513 Size: 552 1119 388 358 447 3074 Fragments arranged by size: 3074 1119 552 447 388 358 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2285 2519 3113 3965 3982 4135 4320 Size: 1255 705 234 594 852 17 153 185 Cuts at: 4320 4765 325 Size: 445 1498 Fragments arranged by size: 1498 1255 852 705 594 445 234 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2253 4184 5896 Size: 274 80 33 51 1443 276 1931 1712 Cuts at: 5896 96 Size: 138 Fragments arranged by size: 1931 1712 1443 276 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3073 3105 5898 5930 3073 Size: 32 2793 32 3081 Fragments arranged by size: 3081 2793 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2315 2756 1316 Size: 999 441 4498 Fragments arranged by size: 4498 999 441 XbaI T'CTAG_A Cuts at: 3274 3274 Size: 5938 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5938 XmaI C'CCGG_G Cuts at: 1870 3263 1870 Size: 1393 4545 XmnI GAAnn'nnTTC Cuts at: 4067 4067 Size: 5938 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2758 2956 3746 276 Size: 53 83 186 936 1224 198 790 2468 Fragments arranged by size: 2468 1224 936 790 198 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI