################################## # NO CUT ENZYMES ( 220 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AluI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII Cfr10I Cfr13I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HaeIII HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MboII MfeI MflI MluI MluNI MnlI MroI MscI MseI MslI MunI MvaI MvnI MwoI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AfaI 84 GTAC GT^AC AlwI 3 GATCC GGATC(4/5) BbvI 8 GCTGC GCAGC(8/12) BcnI 99 CCGGG CC^[GC]GG BsaJI 99 CCGGGG C^C[ACGT][ACGT]GG BsmAI 56 GTCTC GTCTC(1/5) BsmFI 23 GTCCC GGGAC(10/14) BssKI 99 CCGGG ^CC[ACGT]GG BssSI 45 CTCGTG CACGAG(-5/-1) CfoI 75 GCGC GCG^C DpnII 3 GATC ^GATC Fnu4HI 8 GCTGC GC^[ACGT]GC HapII 99 CCGG C^CGG HhaI 75 GCGC GCG^C HinP1I 75 GCGC G^CGC HinfI 54 GAGTC G^A[ACGT]TC HpaII 99 CCGG C^CGG ItaI 8 GCTGC GC^[ACGT]GC MboI 3 GATC ^GATC MspA1I 6 CCGCTG C[AC]G^C[GT]G MspI 99 CCGG C^CGG NciI 99 CCGGG CC^[GC]GG NdeII 3 GATC ^GATC NlaIV 35 GGTTCC GG[ACGT]^[ACGT]CC PflMI 26 CCATCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 54 GAGTC GAGTC(4/5) RsaI 84 GTAC GT^AC Sau3AI 3 GATC ^GATC ScrFI 99 CCGGG CC^[ACGT]GG SfaNI 95 GATGC GCATC(5/9) TaqI 29 TCGA T^CGA TseI 8 GCTGC G^C[AT]GC TthHB8I 29 TCGA T^CGA Van91I 26 CCATCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 70 CCAAAGCGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 3 enzymes) # ################################### AciI 6 CCGC CCGC(-3/-1) 39 CCGC BsiYI 26 CCATCGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 92 CCAGATGCCGG BslI 26 CCATCGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 92 CCAGATGCCGG ######################################### # 3 OR MORE CUT ENZYMES ( 0 enzymes) # #########################################