(Circular) (Six-base) MAPSORT of: pFN21AE1201.seq Check: 6432 from: 1 to: 4831 pFN21AE1201 4831 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2641 278 Size: 53 83 186 936 1105 2468 Fragments arranged by size: 2468 1105 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2174 1937 Size: 237 4594 Acc65I G'GTAC_C Cuts at: 2152 2152 Size: 4831 AclI AA'CG_TT Cuts at: 1563 2958 3331 1563 Size: 1395 373 3063 Fragments arranged by size: 3063 1395 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2894 4278 4546 1632 Size: 1262 1384 268 1917 Fragments arranged by size: 1917 1384 1262 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4634 AflIII A'CryG_T Cuts at: 1203 3730 4707 1203 Size: 2527 977 1327 Fragments arranged by size: 2527 1327 977 AgeI A'CCGG_T Cuts at: 3662 3662 Size: 4831 AhdI GACnn_n'nnGTC Cuts at: 3560 3560 Size: 4831 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4831 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4831 AlwNI CAG_nnn'CTG Cuts at: 2574 4146 2574 Size: 1572 3259 ApaLI G'TGCA_C Cuts at: 2888 4044 2888 Size: 1156 3675 ApoI r'AATT_y Cuts at: 1493 2140 2366 2518 4627 1493 Size: 647 226 152 2109 1697 Fragments arranged by size: 2109 1697 647 226 152 AseI AT'TA_AT Cuts at: 160 1032 3385 160 Size: 872 2353 1606 Fragments arranged by size: 2353 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4831 AvaI C'yCGr_G Cuts at: 1870 1943 2156 1870 Size: 73 213 4545 Fragments arranged by size: 4545 213 73 BamHI G'GATC_C Cuts at: 2161 2528 2161 Size: 367 4464 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2152 3607 618 Size: 325 217 627 365 1455 1842 Fragments arranged by size: 1842 1455 627 365 325 217 BanII G_rGCy'C Cuts at: 1391 2150 1391 Size: 759 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 3860 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3022 3056 1903 Size: 34 1085 34 3678 Fragments arranged by size: 3678 1085 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2723 3933 2723 Size: 1210 3621 BclI T'GATC_A Cuts at: 1548 1548 Size: 4831 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3442 136 Size: 107 122 71 631 605 1770 1525 Fragments arranged by size: 1770 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4826 4826 Size: 4831 BlpI GC'TnA_GC Cuts at: 2248 2248 Size: 4831 Bme1580I G_kGCm'C Cuts at: 1790 2892 4048 1790 Size: 1102 1156 2573 Fragments arranged by size: 2573 1156 1102 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4831 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2562 3520 442 Size: 416 53 83 399 24 1145 958 1753 Fragments arranged by size: 1753 1145 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4831 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3491 1239 Size: 2252 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2956 3821 4119 2956 Size: 865 298 3668 Fragments arranged by size: 3668 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3494 882 Size: 617 1995 2219 Fragments arranged by size: 2219 1995 617 BsaAI yAC'GTr Cuts at: 493 1204 2622 493 Size: 711 1418 2702 Fragments arranged by size: 2702 1418 711 BsaBI GATnn'nnATC Cuts at: 4825 4825 Size: 4831 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2638 3020 3655 275 Size: 53 83 186 936 1105 382 635 1451 Fragments arranged by size: 1451 1105 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3264 3662 3936 4083 4494 3264 Size: 398 274 147 411 3601 Fragments arranged by size: 3601 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4606 Fragments arranged by size: 4606 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4831 BseYI C'CCAG_C Cuts at: 4034 4530 4034 Size: 496 4335 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4258 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2281 3042 4070 1540 Size: 275 186 280 761 1028 2301 Fragments arranged by size: 2301 1028 761 280 275 186 BsiHKAI G_wGCw'C Cuts at: 2892 2977 4048 2892 Size: 85 1071 3675 Fragments arranged by size: 3675 1071 85 BsmI GAATG_Cn' Cuts at: 2342 2435 2342 Size: 93 4738 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2150 2892 2977 4048 1391 Size: 399 360 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 360 85 BspEI T'CCGG_A Cuts at: 4494 4494 Size: 4831 BspHI T'CATG_A Cuts at: 2720 4450 2720 Size: 1730 3101 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2186 844 Size: 1342 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2718 1394 Size: 429 895 3507 Fragments arranged by size: 3507 895 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3326 3500 65 Size: 1183 2078 174 1396 Fragments arranged by size: 2078 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3475 3662 1700 Size: 110 1665 187 2869 Fragments arranged by size: 2869 1665 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4831 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4831 BssSI C'ACGA_G Cuts at: 2050 2891 3903 2050 Size: 841 1012 2978 Fragments arranged by size: 2978 1012 841 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4831 BstBI TT'CG_AA Cuts at: 3635 3635 Size: 4831 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4831 BstYI r'GATC_y Cuts at: 1972 2161 2528 2913 2930 4382 4468 4480 4826 Size: 189 367 385 17 1452 86 12 346 Cuts at: 4826 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 346 189 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4279 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4092 Fragments arranged by size: 4092 683 56 BtsI GCAGTG_nn' Cuts at: 2347 3141 3161 2347 Size: 794 20 4017 Fragments arranged by size: 4017 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4831 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4796 DraI TTT'AAA Cuts at: 2135 2135 Size: 4831 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3838 809 Size: 507 2522 1802 Fragments arranged by size: 2522 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2278 3167 4796 Size: 54 1425 50 275 18 448 889 1629 Cuts at: 4796 8 Size: 43 Fragments arranged by size: 1629 1425 889 448 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2278 1537 Size: 275 466 4090 Fragments arranged by size: 4090 466 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2761 1353 Size: 1408 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3418 3792 3938 387 Size: 1064 138 1829 374 146 1280 Fragments arranged by size: 1829 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2894 3491 4278 4546 1239 Size: 393 1262 597 787 268 1524 Fragments arranged by size: 1524 1262 787 597 393 268 EcoRI G'AATT_C Cuts at: 2140 2518 2140 Size: 378 4453 FspI TGC'GCA Cuts at: 3337 3337 Size: 4831 HaeII r_GCGC'y Cuts at: 1678 3978 1678 Size: 2300 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3472 3504 3546 3578 1373 Size: 32 135 32 1900 32 42 32 2626 Fragments arranged by size: 2626 1900 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2175 2421 669 Size: 988 281 237 246 3079 Fragments arranged by size: 3079 988 281 246 237 HindIII A'AGCT_T Cuts at: 748 748 Size: 4831 HpaI GTT'AAC Cuts at: 2421 2421 Size: 4831 KpnI G_GTAC'C Cuts at: 2156 2156 Size: 4831 MfeI C'AATT_G Cuts at: 2430 2430 Size: 4831 MluI A'CGCG_T Cuts at: 4707 4707 Size: 4831 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3945 4129 1305 Size: 2640 184 2007 Fragments arranged by size: 2640 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3009 Fragments arranged by size: 3009 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2789 3148 3307 518 Size: 648 1623 359 159 2042 Fragments arranged by size: 2042 1623 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2013 2247 2924 4072 4317 1606 Size: 407 234 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 407 245 234 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4279 NdeI CA'TA_TG Cuts at: 387 387 Size: 4831 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4831 NotI GC'GGCC_GC Cuts at: 2278 2278 Size: 4831 NspI r_CATG'y Cuts at: 1132 1229 2189 3734 1132 Size: 97 960 1545 2229 Fragments arranged by size: 2229 1545 960 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4831 PciI A'CATG_T Cuts at: 3730 3730 Size: 4831 PflMI CCAn_nnn'nTGG Cuts at: 1108 2037 1108 Size: 929 3902 PmeI GTTT'AAAC Cuts at: 2135 2135 Size: 4831 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2900 2932 4413 4445 2900 Size: 32 1481 32 3286 Fragments arranged by size: 3286 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4831 PsiI TTA'TAA Cuts at: 2401 2401 Size: 4831 PstI C_TGCA'G Cuts at: 830 2183 830 Size: 1353 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4831 SalI G'TCGA_C Cuts at: 1936 2173 1936 Size: 237 4594 SbfI CC_TGCA'GG Cuts at: 2183 2183 Size: 4831 ScaI AGT'ACT Cuts at: 3079 4791 3079 Size: 1712 3119 SfcI C'TryA_G Cuts at: 826 1045 2179 3314 3995 4186 826 Size: 219 1134 1135 681 191 1471 Fragments arranged by size: 1471 1135 1134 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4831 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4831 SmaI CCC'GGG Cuts at: 1872 2158 1872 Size: 286 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2935 3836 4098 820 Size: 197 926 992 901 262 1553 Fragments arranged by size: 1553 992 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2622 493 Size: 2129 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4831 SphI G_CATG'C Cuts at: 1132 2189 1132 Size: 1057 3774 SspI AAT'ATT Cuts at: 5 52 2755 5 Size: 47 2703 2081 Fragments arranged by size: 2703 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4831 StyI C'CwwG_G Cuts at: 513 1065 2270 513 Size: 552 1205 3074 Fragments arranged by size: 3074 1205 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2858 2875 3028 3213 3658 325 Size: 1255 1278 17 153 185 445 1498 Fragments arranged by size: 1498 1278 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3077 4789 96 Size: 274 80 33 51 1443 1100 1712 138 Fragments arranged by size: 1712 1443 1100 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4791 4823 4791 Size: 32 4799 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4831 XbaI T'CTAG_A Cuts at: 2167 2167 Size: 4831 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2082 2082 Size: 4831 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4831 XmaI C'CCGG_G Cuts at: 1870 2156 1870 Size: 286 4545 XmnI GAAnn'nnTTC Cuts at: 2960 2960 Size: 4831 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2639 276 Size: 53 83 186 936 1105 2468 Fragments arranged by size: 2468 1105 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI