(Linear) (Six-base) MAPSORT of: KIEE2163.seq Check: 2404 from: 1 to: 2646 KIEE2163.orf With 182 enzymes: SgfI * July 15, 2010 18:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1335 2646 Size: 1335 1311 Acc65I G'GTAC_C Cuts at: 0 1096 2138 2646 Size: 1096 1042 508 Fragments arranged by size: 1096 1042 508 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 604 1411 2563 2646 Size: 604 807 1152 83 Fragments arranged by size: 1152 807 604 83 AfeI AGC'GCT Cuts at: 0 2287 2646 Size: 2287 359 AflIII A'CryG_T Cuts at: 0 38 1166 1798 2646 Size: 38 1128 632 848 Fragments arranged by size: 1128 848 632 38 AhdI GACnn_n'nnGTC Cuts at: 0 1782 2646 Size: 1782 864 AlwNI CAG_nnn'CTG Cuts at: 0 807 1680 2319 2353 2646 Size: 807 873 639 34 293 Fragments arranged by size: 873 807 639 293 34 ApaLI G'TGCA_C Cuts at: 0 1315 2646 Size: 1315 1331 ApoI r'AATT_y Cuts at: 0 1081 2646 Size: 1081 1565 AvaI C'yCGr_G Cuts at: 0 154 1299 2512 2531 2646 Size: 154 1145 1213 19 115 Fragments arranged by size: 1213 1145 154 115 19 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1080 1113 2646 Size: 1080 33 1533 Fragments arranged by size: 1533 1080 33 BanI G'GyrC_C Cuts at: 0 1096 1287 1846 2055 2138 2450 2646 Size: 1096 191 559 209 83 312 196 Fragments arranged by size: 1096 559 312 209 196 191 83 BanII G_rGCy'C Cuts at: 0 140 1541 2157 2576 2646 Size: 140 1401 616 419 70 Fragments arranged by size: 1401 616 419 140 70 BbeI G_GCGC'C Cuts at: 0 1291 2059 2646 Size: 1291 768 587 Fragments arranged by size: 1291 768 587 BbsI GAAGACnn'nnnn_ Cuts at: 0 870 1801 2646 Size: 870 931 845 Fragments arranged by size: 931 870 845 BbvCI CC'TCA_GC Cuts at: 0 1106 2646 Size: 1106 1540 BglII A'GATC_T Cuts at: 0 1715 2646 Size: 1715 931 Bme1580I G_kGCm'C Cuts at: 0 982 1319 2238 2646 Size: 982 337 919 408 Fragments arranged by size: 982 919 408 337 BmgBI CAC'GTC Cuts at: 0 1002 1799 2646 Size: 1002 797 847 Fragments arranged by size: 1002 847 797 BmrI ACTGGGnnnn_n' Cuts at: 0 185 295 2161 2646 Size: 185 110 1866 485 Fragments arranged by size: 1866 485 185 110 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 154 331 1488 2224 2617 2646 Size: 154 177 1157 736 393 29 Fragments arranged by size: 1157 736 393 177 154 29 Bpu10I CC'TnA_GC Cuts at: 0 1106 1888 2646 Size: 1106 782 758 Fragments arranged by size: 1106 782 758 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 193 409 529 2646 Size: 193 216 120 2117 Fragments arranged by size: 2117 216 193 120 BsaI GGTCTCn'nnnn_ Cuts at: 0 1906 2646 Size: 1906 740 BsaAI yAC'GTr Cuts at: 0 39 624 1320 2646 Size: 39 585 696 1326 Fragments arranged by size: 1326 696 585 39 BsaHI Gr'CG_yC Cuts at: 0 443 764 1288 2056 2646 Size: 443 321 524 768 590 Fragments arranged by size: 768 590 524 443 321 BsaWI w'CCGG_w Cuts at: 0 20 2646 Size: 20 2626 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 15 45 1138 1168 1783 1813 1915 1945 Size: 15 30 1093 30 615 30 102 30 Cuts at: 1945 1990 2020 2646 Size: 45 30 626 Fragments arranged by size: 1093 626 615 102 45 30 30 30 30 30 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1390 1546 1741 1810 1936 2646 Size: 1390 156 195 69 126 710 Fragments arranged by size: 1390 710 195 156 126 69 BseYI C'CCAG_C Cuts at: 0 1114 1903 2646 Size: 1114 789 743 Fragments arranged by size: 1114 789 743 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 238 550 764 769 799 850 1342 1633 Size: 238 312 214 5 30 51 492 291 Cuts at: 1633 1711 2395 2646 Size: 78 684 251 Fragments arranged by size: 684 492 312 291 251 238 214 78 51 30 5 BsiHKAI G_wGCw'C Cuts at: 0 659 1319 1541 1556 2576 2646 Size: 659 660 222 15 1020 70 Fragments arranged by size: 1020 660 659 222 70 15 BsmI GAATG_Cn' Cuts at: 0 1259 2646 Size: 1259 1387 Bsp1286I G_dGCh'C Cuts at: 0 140 659 982 1319 1541 1556 2157 2238 Size: 140 519 323 337 222 15 601 81 Cuts at: 2238 2576 2646 Size: 338 70 Fragments arranged by size: 601 519 338 337 323 222 140 81 70 15 BspEI T'CCGG_A Cuts at: 0 20 2646 Size: 20 2626 BspHI T'CATG_A Cuts at: 0 1577 2646 Size: 1577 1069 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1335 1462 2361 2646 Size: 1335 127 899 285 Fragments arranged by size: 1335 899 285 127 BsrBI CCG'CTC Cuts at: 0 1740 2646 Size: 1740 906 BsrFI r'CCGG_y Cuts at: 0 766 2206 2646 Size: 766 1440 440 Fragments arranged by size: 1440 766 440 BsrGI T'GTAC_A Cuts at: 0 1619 2646 Size: 1619 1027 BstAPI GCAn_nnn'nTGC Cuts at: 0 1638 1680 2646 Size: 1638 42 966 Fragments arranged by size: 1638 966 42 BstEII G'GTnAC_C Cuts at: 0 2391 2646 Size: 2391 255 BstXI CCAn_nnnn'nTGG Cuts at: 0 1272 1512 2364 2484 2646 Size: 1272 240 852 120 162 Fragments arranged by size: 1272 852 240 162 120 BstYI r'GATC_y Cuts at: 0 1715 1925 2646 Size: 1715 210 721 Fragments arranged by size: 1715 721 210 BtgI C'CryG_G Cuts at: 0 387 890 1410 1444 2117 2646 Size: 387 503 520 34 673 529 Fragments arranged by size: 673 529 520 503 387 34 BtsI GCAGTG_nn' Cuts at: 0 781 1289 1678 1850 2646 Size: 781 508 389 172 796 Fragments arranged by size: 796 781 508 389 172 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 369 404 2646 Size: 369 35 2242 Fragments arranged by size: 2242 369 35 DraIII CAC_nnn'GTG Cuts at: 0 1638 2646 Size: 1638 1008 DrdI GACnn_nn'nnGTC Cuts at: 0 732 1587 2646 Size: 732 855 1059 Fragments arranged by size: 1059 855 732 EaeI y'GGCC_r Cuts at: 0 302 390 719 1214 1941 2646 Size: 302 88 329 495 727 705 Fragments arranged by size: 727 705 495 329 302 88 EarI CTCTTCn'nnn_ Cuts at: 0 1468 1546 2646 Size: 1468 78 1100 Fragments arranged by size: 1468 1100 78 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 160 2646 Size: 160 2486 EcoICRI GAG'CTC Cuts at: 0 1539 2574 2646 Size: 1539 1035 72 Fragments arranged by size: 1539 1035 72 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 154 331 604 1411 1488 2224 2563 2617 Size: 154 177 273 807 77 736 339 54 Cuts at: 2617 2646 Size: 29 Fragments arranged by size: 807 736 339 273 177 154 77 54 29 EcoO109I rG'GnC_Cy Cuts at: 0 171 804 1008 1734 1749 2094 2628 2646 Size: 171 633 204 726 15 345 534 18 Fragments arranged by size: 726 633 534 345 204 171 18 15 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 894 2202 2646 Size: 894 1308 444 Fragments arranged by size: 1308 894 444 FspI TGC'GCA Cuts at: 0 539 2646 Size: 539 2107 HaeII r_GCGC'y Cuts at: 0 915 1291 2033 2059 2289 2646 Size: 915 376 742 26 230 357 Fragments arranged by size: 915 742 376 357 230 26 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 15 47 1768 1800 2206 2238 2646 Size: 15 32 1721 32 406 32 408 Fragments arranged by size: 1721 408 406 32 32 32 15 HincII GTy'rAC Cuts at: 0 435 2646 Size: 435 2211 HindIII A'AGCT_T Cuts at: 0 871 2646 Size: 871 1775 KasI G'GCGC_C Cuts at: 0 1287 2055 2646 Size: 1287 768 591 Fragments arranged by size: 1287 768 591 KpnI G_GTAC'C Cuts at: 0 1100 2142 2646 Size: 1100 1042 504 Fragments arranged by size: 1100 1042 504 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1616 2021 2033 2646 Size: 1616 405 12 613 Fragments arranged by size: 1616 613 405 12 MscI TGG'CCA Cuts at: 0 304 721 1943 2646 Size: 304 417 1222 703 Fragments arranged by size: 1222 703 417 304 MslI CAynn'nnrTG Cuts at: 0 943 964 985 991 1270 1360 1576 2646 Size: 943 21 21 6 279 90 216 1070 Fragments arranged by size: 1070 943 279 216 90 21 21 6 MspA1I CmG'CkG Cuts at: 0 615 1326 2119 2249 2646 Size: 615 711 793 130 397 Fragments arranged by size: 793 711 615 397 130 NaeI GCC'GGC Cuts at: 0 768 2208 2646 Size: 768 1440 438 Fragments arranged by size: 1440 768 438 NarI GG'CG_CC Cuts at: 0 1288 2056 2646 Size: 1288 768 590 Fragments arranged by size: 1288 768 590 NgoMIV G'CCGG_C Cuts at: 0 766 2206 2646 Size: 766 1440 440 Fragments arranged by size: 1440 766 440 NspI r_CATG'y Cuts at: 0 17 366 778 1170 1182 1878 2646 Size: 17 349 412 392 12 696 768 Fragments arranged by size: 768 696 412 392 349 17 12 PciI A'CATG_T Cuts at: 0 1166 2646 Size: 1166 1480 PflMI CCAn_nnn'nTGG Cuts at: 0 297 807 2319 2646 Size: 297 510 1512 327 Fragments arranged by size: 1512 510 327 297 PfoI T'CCnGG_A Cuts at: 0 308 800 1654 2646 Size: 308 492 854 992 Fragments arranged by size: 992 854 492 308 PmlI CAC'GTG Cuts at: 0 39 1320 2646 Size: 39 1281 1326 Fragments arranged by size: 1326 1281 39 PpuMI rG'GwC_Cy Cuts at: 0 171 804 1734 1749 2094 2628 2646 Size: 171 633 930 15 345 534 18 Fragments arranged by size: 930 633 534 345 171 18 15 PshAI GACnn'nnGTC Cuts at: 0 1766 2646 Size: 1766 880 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1963 1995 2646 Size: 1963 32 651 Fragments arranged by size: 1963 651 32 PstI C_TGCA'G Cuts at: 0 595 1676 2646 Size: 595 1081 970 Fragments arranged by size: 1081 970 595 PvuII CAG'CTG Cuts at: 0 1326 2646 Size: 1326 1320 RsrII CG'GwC_CG Cuts at: 0 2121 2646 Size: 2121 525 SacI G_AGCT'C Cuts at: 0 1541 2576 2646 Size: 1541 1035 70 Fragments arranged by size: 1541 1035 70 SacII CC_GC'GG Cuts at: 0 2120 2646 Size: 2120 526 SanDI GG'GwC_CC Cuts at: 0 171 2646 Size: 171 2475 SapI GCTCTTCn'nnn_ Cuts at: 0 1546 2646 Size: 1546 1100 ScaI AGT'ACT Cuts at: 0 472 1708 2646 Size: 472 1236 938 Fragments arranged by size: 1236 938 472 SexAI A'CCwGG_T Cuts at: 0 1634 2646 Size: 1634 1012 SfcI C'TryA_G Cuts at: 0 591 1672 2646 Size: 591 1081 974 Fragments arranged by size: 1081 974 591 SfoI GGC'GCC Cuts at: 0 1289 2057 2646 Size: 1289 768 589 Fragments arranged by size: 1289 768 589 SmlI C'TyrA_G Cuts at: 0 208 424 544 2646 Size: 208 216 120 2102 Fragments arranged by size: 2102 216 208 120 SphI G_CATG'C Cuts at: 0 17 778 1878 2646 Size: 17 761 1100 768 Fragments arranged by size: 1100 768 761 17 StuI AGG'CCT Cuts at: 0 131 2646 Size: 131 2515 StyI C'CwwG_G Cuts at: 0 1787 2090 2385 2420 2646 Size: 1787 303 295 35 226 Fragments arranged by size: 1787 303 295 226 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2290 2646 Size: 2290 356 TatI w'GTAC_w Cuts at: 0 470 1619 1706 2180 2646 Size: 470 1149 87 474 466 Fragments arranged by size: 1149 474 470 466 87 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1138 1170 1189 1221 1783 1815 2646 Size: 1138 32 19 32 562 32 831 Fragments arranged by size: 1138 831 562 32 32 32 19 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 750 1936 2316 2646 Size: 750 1186 380 330 Fragments arranged by size: 1186 750 380 330 XmnI GAAnn'nnTTC Cuts at: 0 1084 1147 1708 2646 Size: 1084 63 561 938 Fragments arranged by size: 1084 938 561 63 Enzymes that do cut: AarI Acc65I AcuI AfeI AflIII AhdI AlwNI ApaLI ApoI AvaI BaeI BanI BanII BbeI BbsI BbvCI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI BstAPI BstEII BstXI BstYI BtgI BtsI CspCI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI PmlI PpuMI PshAI PsrI PstI PvuII RsrII SacI SacII SanDI SapI ScaI SexAI SfcI SfoI SmlI SphI StuI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AatII AccI AclI AflII AgeI AleI AlfI AloI ApaI AscI AseI AsiSI AvrII BamHI BcgI BciVI BclI BfrBI BglI BlpI BmtI BplI BsaBI BsiEI BsiWI BsmBI BsrDI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI ClaI DraI EagI EcoNI EcoRI EcoRV FseI FspAI HpaI MfeI MluI NcoI NdeI NheI NotI NruI NsiI PacI PasI PmeI PpiI PsiI PspOMI PspXI PvuI SalI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmaI ZraI