################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII Acc65I AccIII AclI AflIII AgeI Alw44I ApaLI AscI AseI AsnI Asp718I AspI BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BclI BglII BlpI Bpu1102I BpuAI BsaAI BsaBI BseAI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BssHII Bst1107I BstBI BstZ17I CelII ClaI CpoI EcoRI EcoRV EcoT22I FbaI HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AcsI 1889 AAATTC [GA]^AATT[CT] AcyI 621 GGCGTC G[GA]^CG[CT]C AflII 249 CTTAAG C^TTAAG AocI 188 CCTCAGG CC^T[ACGT]AGG Aor51HI 903 AGCGCT AGC^GCT ApoI 1889 AAATTC [GA]^AATT[CT] Asp700I 1815 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 1395 TGCGCA TGC^GCA AvrII 58 CCTAGG C^CTAGG BciVI 2459 GTATCC GTATCC(6/5) BfrI 249 CTTAAG C^TTAAG BlnI 58 CCTAGG C^CTAGG BsaHI 621 GGCGTC G[GA]^CG[CT]C BsaI 510 GGTCTC GGTCTC(1/5) BsaWI 32 ACCGGA [AT]^CCGG[AT] BsiEI 1271 CGGCCG CG[GA][CT]^CG Bsp1407I 2226 TGTACA T^GTACA BspMI 2012 ACCTGC ACCTGC(4/8) BsrGI 2226 TGTACA T^GTACA Bsu36I 188 CCTCAGG CC^T[ACGT]AGG DraI 333 TTTAAA TTT^AAA DraIII 1228 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 1271 CGGCCG C^GGCCG EclXI 1271 CGGCCG C^GGCCG Eco47III 903 AGCGCT AGC^GCT Eco52I 1271 CGGCCG C^GGCCG Eco81I 188 CCTCAGG CC^T[ACGT]AGG FseI 1272 GGCCGGCC GGCCGG^CC FspI 1395 TGCGCA TGC^GCA Hin1I 621 GGCGTC G[GA]^CG[CT]C HincII 1222 GTCAAC GT[CT]^[GA]AC HindII 1222 GTCAAC GT[CT]^[GA]AC PaeR7I 2679 CTCGAG C^TCGAG PshAI 2749 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SfiI 1246 GGCCTGCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspBI 2226 TGTACA T^GTACA XhoI 2679 CTCGAG C^TCGAG XmnI 1815 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AhdI 630 GACCTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 642 GACGGAAAGTC AspEI 630 GACCTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 642 GACGGAAAGTC BanI 1092 GGTGCC G^G[CT][GA]CC 2559 GGTGCC BsgI 1237 GTGCAG GTGCAG(16/14) 2035 GTGCAG BsmI 558 GAATGC GAATGC(1/-1) 1073 GCATTC BsrBI 888 CCGCTC CCGCTC(-3/-3) 1342 CCGCTC BsrDI 1498 CATTGC GCAATG(2/0) 553 GCAATG BssSI 1174 CACGAG CACGAG(-5/-1) 1549 CTCGTG BstEII 297 GGTCACC G^GT[ACGT]ACC 450 GGTCACC BstPI 297 GGTCACC G^GT[ACGT]ACC 450 GGTCACC BstXI 971 CCACGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2463 CCATGTCCATGG BstYI 747 GGATCT [GA]^GATC[CT] 1754 AGATCC DrdI 771 GACATCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2575 GACTGCCTAGTC Eam1105I 630 GACCTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 642 GACGGAAAGTC EarI 2439 CTCTTC CTCTTC(1/4) 897 GAAGAG EcoO65I 297 GGTCACC G^GT[ACGT]ACC 450 GGTCACC Ksp632I 2439 CTCTTC CTCTTC(1/4) 897 GAAGAG MflI 747 GGATCT [GA]^GATC[CT] 1754 AGATCC MseI 250 TTAA T^TAA 334 TTAA NcoI 2469 CCATGG C^CATGG 2594 CCATGG PpuMI 595 AGGTCCC [GA]G^G[AT]CC[CT] 874 AGGTCCC SexAI 592 ACCAGGT A^CC[AT]GGT 871 ACCAGGT SphI 520 GCATGC GCATG^C 1181 GCATGC TfiI 290 GATTC G^A[AT]TC 2518 GATTC Tru9I 250 TTAA T^TAA 334 TTAA XcmI 971 CCACGTCCTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2469 CCATGGATTTTATGG XhoII 747 GGATCT [GA]^GATC[CT] 1754 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AccI AccII AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BanII BbvI BcnI BfaI BglI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XmaCI XmaI