(Linear) (Six-base) MAPSORT of: KIEE2109.seq Check: 3297 from: 1 to: 2790 KIEE2109.orf With 182 enzymes: SgfI * July 15, 2010 18:55 .. AccI GT'mk_AC Cuts at: 0 1040 1322 2777 2790 Size: 1040 282 1455 13 Fragments arranged by size: 1455 1040 282 13 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 569 1806 2099 2434 2735 2790 Size: 569 1237 293 335 301 55 Fragments arranged by size: 1237 569 335 301 293 55 AfeI AGC'GCT Cuts at: 0 905 2790 Size: 905 1885 AflII C'TTAA_G Cuts at: 0 249 2790 Size: 249 2541 AhdI GACnn_n'nnGTC Cuts at: 0 635 647 2790 Size: 635 12 2143 Fragments arranged by size: 2143 635 12 AleI CACnn'nnGTG Cuts at: 0 2275 2790 Size: 2275 515 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 256 2575 2790 Size: 256 2319 215 Fragments arranged by size: 2319 256 215 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2364 2396 2790 Size: 2364 32 394 Fragments arranged by size: 2364 394 32 AlwNI CAG_nnn'CTG Cuts at: 0 480 1437 2043 2173 2290 2549 2790 Size: 480 957 606 130 117 259 241 Fragments arranged by size: 957 606 480 259 241 130 117 ApaI G_GGCC'C Cuts at: 0 699 1167 1258 1424 2790 Size: 699 468 91 166 1366 Fragments arranged by size: 1366 699 468 166 91 ApoI r'AATT_y Cuts at: 0 1889 2790 Size: 1889 901 AvaI C'yCGr_G Cuts at: 0 766 1308 1417 2107 2643 2679 2790 Size: 766 542 109 690 536 36 111 Fragments arranged by size: 766 690 542 536 111 109 36 AvrII C'CTAG_G Cuts at: 0 58 2790 Size: 58 2732 BanI G'GyrC_C Cuts at: 0 1092 2559 2790 Size: 1092 1467 231 Fragments arranged by size: 1467 1092 231 BanII G_rGCy'C Cuts at: 0 99 393 699 1167 1258 1418 1424 1550 Size: 99 294 306 468 91 160 6 126 Cuts at: 1550 2003 2239 2790 Size: 453 236 551 Fragments arranged by size: 551 468 453 306 294 236 160 126 99 91 6 BciVI GTATCCnnnnn_n' Cuts at: 0 2470 2790 Size: 2470 320 BglI GCCn_nnn'nGGC Cuts at: 0 1109 1253 1775 2790 Size: 1109 144 522 1015 Fragments arranged by size: 1109 1015 522 144 Bme1580I G_kGCm'C Cuts at: 0 699 1097 1167 1258 1424 2790 Size: 699 398 70 91 166 1366 Fragments arranged by size: 1366 699 398 166 91 70 BmgBI CAC'GTC Cuts at: 0 965 974 2790 Size: 965 9 1816 Fragments arranged by size: 1816 965 9 BmrI ACTGGGnnnn_n' Cuts at: 0 490 2250 2790 Size: 490 1760 540 Fragments arranged by size: 1760 540 490 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 329 482 1148 1189 1213 1318 1815 2124 Size: 329 153 666 41 24 105 497 309 Cuts at: 2124 2198 2790 Size: 74 592 Fragments arranged by size: 666 592 497 329 309 153 105 74 41 24 Bpu10I CC'TnA_GC Cuts at: 0 754 2790 Size: 754 2036 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 163 645 654 1735 2790 Size: 163 482 9 1081 1055 Fragments arranged by size: 1081 1055 482 163 9 BsaI GGTCTCn'nnnn_ Cuts at: 0 516 2790 Size: 516 2274 BsaHI Gr'CG_yC Cuts at: 0 622 2790 Size: 622 2168 BsaWI w'CCGG_w Cuts at: 0 32 2790 Size: 32 2758 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2233 2236 2263 2266 2364 2394 2790 Size: 2233 3 27 3 98 30 396 Fragments arranged by size: 2233 396 98 30 27 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 197 209 1315 1340 1558 2241 2309 2760 Size: 197 12 1106 25 218 683 68 451 Cuts at: 2760 2790 Size: 30 Fragments arranged by size: 1106 683 451 218 197 68 30 25 12 BseYI C'CCAG_C Cuts at: 0 1079 1430 2173 2390 2790 Size: 1079 351 743 217 400 Fragments arranged by size: 1079 743 400 351 217 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1258 2056 2790 Size: 1258 798 734 Fragments arranged by size: 1258 798 734 BsiEI CG_ry'CG Cuts at: 0 1274 2790 Size: 1274 1516 BsiHKAI G_wGCw'C Cuts at: 0 1175 1199 1418 1550 2003 2790 Size: 1175 24 219 132 453 787 Fragments arranged by size: 1175 787 453 219 132 24 BsmI GAATG_Cn' Cuts at: 0 564 1073 2790 Size: 564 509 1717 Fragments arranged by size: 1717 564 509 Bsp1286I G_dGCh'C Cuts at: 0 99 393 699 1097 1167 1175 1199 1258 Size: 99 294 306 398 70 8 24 59 Cuts at: 1258 1418 1424 1550 2003 2239 2790 Size: 160 6 126 453 236 551 Fragments arranged by size: 551 453 398 306 294 236 160 126 99 70 59 24 8 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2021 2790 Size: 2021 769 BsrBI CCG'CTC Cuts at: 0 890 1344 2790 Size: 890 454 1446 Fragments arranged by size: 1446 890 454 BsrDI GCAATG_nn' Cuts at: 0 560 1497 2790 Size: 560 937 1293 Fragments arranged by size: 1293 937 560 BsrFI r'CCGG_y Cuts at: 0 1011 1143 1269 1273 1615 2049 2790 Size: 1011 132 126 4 342 434 741 Fragments arranged by size: 1011 741 434 342 132 126 4 BsrGI T'GTAC_A Cuts at: 0 2226 2790 Size: 2226 564 BssSI C'ACGA_G Cuts at: 0 1174 1549 2790 Size: 1174 375 1241 Fragments arranged by size: 1241 1174 375 BstAPI GCAn_nnn'nTGC Cuts at: 0 160 559 1248 1499 2043 2308 2790 Size: 160 399 689 251 544 265 482 Fragments arranged by size: 689 544 482 399 265 251 160 BstEII G'GTnAC_C Cuts at: 0 297 450 2790 Size: 297 153 2340 Fragments arranged by size: 2340 297 153 BstXI CCAn_nnnn'nTGG Cuts at: 0 978 2470 2790 Size: 978 1492 320 Fragments arranged by size: 1492 978 320 BstYI r'GATC_y Cuts at: 0 747 1754 2790 Size: 747 1007 1036 Fragments arranged by size: 1036 1007 747 Bsu36I CC'TnA_GG Cuts at: 0 189 2790 Size: 189 2601 BtgI C'CryG_G Cuts at: 0 717 2087 2469 2594 2744 2790 Size: 717 1370 382 125 150 46 Fragments arranged by size: 1370 717 382 150 125 46 BtsI GCAGTG_nn' Cuts at: 0 2559 2790 Size: 2559 231 DraI TTT'AAA Cuts at: 0 335 2790 Size: 335 2455 DraIII CAC_nnn'GTG Cuts at: 0 1233 2790 Size: 1233 1557 DrdI GACnn_nn'nnGTC Cuts at: 0 777 2581 2790 Size: 777 1804 209 Fragments arranged by size: 1804 777 209 EaeI y'GGCC_r Cuts at: 0 1013 1271 2051 2790 Size: 1013 258 780 739 Fragments arranged by size: 1013 780 739 258 EagI C'GGCC_G Cuts at: 0 1271 2790 Size: 1271 1519 EarI CTCTTCn'nnn_ Cuts at: 0 892 2445 2790 Size: 892 1553 345 Fragments arranged by size: 1553 892 345 EcoICRI GAG'CTC Cuts at: 0 1416 1548 2001 2790 Size: 1416 132 453 789 Fragments arranged by size: 1416 789 453 132 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 329 482 569 1148 1189 1213 1318 1806 Size: 329 153 87 579 41 24 105 488 Cuts at: 1806 1815 2099 2124 2198 2434 2735 2790 Size: 9 284 25 74 236 301 55 Fragments arranged by size: 579 488 329 301 284 236 153 105 87 74 55 41 25 24 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 383 1734 2213 2790 Size: 383 1351 479 577 Fragments arranged by size: 1351 577 479 383 EcoO109I rG'GnC_Cy Cuts at: 0 55 596 695 696 875 1163 1164 1255 Size: 55 541 99 1 179 288 1 91 Cuts at: 1255 1369 1421 2790 Size: 114 52 1369 Fragments arranged by size: 1369 541 288 179 114 99 91 55 52 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 409 1776 2790 Size: 409 1367 1014 Fragments arranged by size: 1367 1014 409 FseI GG_CCGG'CC Cuts at: 0 1277 2790 Size: 1277 1513 FspI TGC'GCA Cuts at: 0 1397 2790 Size: 1397 1393 FspAI rTGC'GCAy Cuts at: 0 1397 2790 Size: 1397 1393 HaeII r_GCGC'y Cuts at: 0 907 1141 1526 2790 Size: 907 234 385 1264 Fragments arranged by size: 1264 907 385 234 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 192 224 254 281 286 313 470 502 Size: 192 32 30 27 5 27 157 32 Cuts at: 502 580 612 621 633 653 665 2790 Size: 78 32 9 12 20 12 2125 Fragments arranged by size: 2125 192 157 78 32 32 32 30 27 27 20 12 12 9 5 HincII GTy'rAC Cuts at: 0 1224 2790 Size: 1224 1566 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 737 2790 Size: 737 2053 MslI CAynn'nnrTG Cuts at: 0 1234 1391 2275 2468 2653 2790 Size: 1234 157 884 193 185 137 Fragments arranged by size: 1234 884 193 185 157 137 MspA1I CmG'CkG Cuts at: 0 434 921 1070 1191 1437 1508 1854 2170 Size: 434 487 149 121 246 71 346 316 Cuts at: 2170 2275 2487 2708 2753 2790 Size: 105 212 221 45 37 Fragments arranged by size: 487 434 346 316 246 221 212 149 121 105 71 45 37 NaeI GCC'GGC Cuts at: 0 1013 1145 1271 1275 2051 2790 Size: 1013 132 126 4 776 739 Fragments arranged by size: 1013 776 739 132 126 4 NcoI C'CATG_G Cuts at: 0 2469 2594 2790 Size: 2469 125 196 Fragments arranged by size: 2469 196 125 NgoMIV G'CCGG_C Cuts at: 0 1011 1143 1269 1273 2049 2790 Size: 1011 132 126 4 776 741 Fragments arranged by size: 1011 776 741 132 126 4 NspI r_CATG'y Cuts at: 0 318 524 1185 1942 2790 Size: 318 206 661 757 848 Fragments arranged by size: 848 757 661 318 206 PasI CC'CwG_GG Cuts at: 0 684 2790 Size: 684 2106 PflMI CCAn_nnn'nTGG Cuts at: 0 379 1233 1437 2790 Size: 379 854 204 1353 Fragments arranged by size: 1353 854 379 204 PfoI T'CCnGG_A Cuts at: 0 103 1893 2214 2790 Size: 103 1790 321 576 Fragments arranged by size: 1790 576 321 103 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2364 2396 2790 Size: 2364 32 394 Fragments arranged by size: 2364 394 32 PpuMI rG'GwC_Cy Cuts at: 0 596 875 2790 Size: 596 279 1915 Fragments arranged by size: 1915 596 279 PshAI GACnn'nnGTC Cuts at: 0 2753 2790 Size: 2753 37 PspOMI G'GGCC_C Cuts at: 0 695 1163 1254 1420 2790 Size: 695 468 91 166 1370 Fragments arranged by size: 1370 695 468 166 91 PspXI vC'TCGA_Gb Cuts at: 0 2679 2790 Size: 2679 111 PstI C_TGCA'G Cuts at: 0 1211 1522 2048 2492 2554 2790 Size: 1211 311 526 444 62 236 Fragments arranged by size: 1211 526 444 311 236 62 PvuII CAG'CTG Cuts at: 0 434 1070 1191 1437 2170 2275 2487 2790 Size: 434 636 121 246 733 105 212 303 Fragments arranged by size: 733 636 434 303 246 212 121 105 SacI G_AGCT'C Cuts at: 0 1418 1550 2003 2790 Size: 1418 132 453 787 Fragments arranged by size: 1418 787 453 132 SexAI A'CCwGG_T Cuts at: 0 592 871 2790 Size: 592 279 1919 Fragments arranged by size: 1919 592 279 SfcI C'TryA_G Cuts at: 0 515 690 1207 1518 2044 2488 2550 2790 Size: 515 175 517 311 526 444 62 240 Fragments arranged by size: 526 517 515 444 311 240 175 62 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1253 2790 Size: 1253 1537 SmaI CCC'GGG Cuts at: 0 768 1310 2109 2790 Size: 768 542 799 681 Fragments arranged by size: 799 768 681 542 SmlI C'TyrA_G Cuts at: 0 178 249 633 660 1750 2679 2790 Size: 178 71 384 27 1090 929 111 Fragments arranged by size: 1090 929 384 178 111 71 27 SphI G_CATG'C Cuts at: 0 524 1185 2790 Size: 524 661 1605 Fragments arranged by size: 1605 661 524 StuI AGG'CCT Cuts at: 0 1133 1217 1729 1819 2790 Size: 1133 84 512 90 971 Fragments arranged by size: 1133 971 512 90 84 StyI C'CwwG_G Cuts at: 0 58 977 1365 2469 2594 2790 Size: 58 919 388 1104 125 196 Fragments arranged by size: 1104 919 388 196 125 58 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 316 1303 2790 Size: 316 987 1487 Fragments arranged by size: 1487 987 316 TatI w'GTAC_w Cuts at: 0 2226 2790 Size: 2226 564 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 54 86 126 158 444 476 2233 2265 Size: 54 32 40 32 286 32 1757 32 Cuts at: 2265 2401 2433 2790 Size: 136 32 357 Fragments arranged by size: 1757 357 286 136 54 40 32 32 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 978 2476 2790 Size: 978 1498 314 Fragments arranged by size: 1498 978 314 XhoI C'TCGA_G Cuts at: 0 2679 2790 Size: 2679 111 XmaI C'CCGG_G Cuts at: 0 766 1308 2107 2790 Size: 766 542 799 683 Fragments arranged by size: 799 766 683 542 XmnI GAAnn'nnTTC Cuts at: 0 1819 2790 Size: 1819 971 Enzymes that do cut: AccI AcuI AfeI AflII AhdI AleI AlfI AloI AlwNI ApaI ApoI AvaI AvrII BanI BanII BciVI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DraI DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI FspI FspAI HaeII Hin4I HincII MmeI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PfoI PpiI PpuMI PshAI PspOMI PspXI PstI PvuII SacI SexAI SfcI SfiI SmaI SmlI SphI StuI StyI TaqII TatI TstI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AflIII AgeI ApaLI AscI AseI AsiSI BaeI BamHI BbeI BbsI BbvCI BcgI BclI BfrBI BglII BlpI BmtI BplI BsaAI BsaBI BsiWI BsmBI BspEI BspHI BssHII BstBI BstZ17I BtgZI ClaI CspCI EciI EcoRI EcoRV HindIII HpaI KasI KpnI MfeI MluI MscI NarI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PsiI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI ZraI