(Circular) (Six-base) MAPSORT of: pFN21AE0179.seq Check: 7231 from: 1 to: 5731 pFN21AE0179 5731 bp With 182 enzymes: SgfI * October 20, 2009 10:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3541 278 Size: 53 83 186 936 2005 2468 Fragments arranged by size: 2468 2005 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2601 3074 1937 Size: 664 473 4594 Fragments arranged by size: 4594 664 473 Acc65I G'GTAC_C Cuts at: 3052 3052 Size: 5731 AclI AA'CG_TT Cuts at: 1563 3858 4231 1563 Size: 2295 373 3063 Fragments arranged by size: 3063 2295 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3794 5178 5446 1632 Size: 2162 1384 268 1917 Fragments arranged by size: 2162 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5534 AflIII A'CryG_T Cuts at: 1203 4630 5607 1203 Size: 3427 977 1327 Fragments arranged by size: 3427 1327 977 AgeI A'CCGG_T Cuts at: 4562 4562 Size: 5731 AhdI GACnn_n'nnGTC Cuts at: 4460 4460 Size: 5731 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5731 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5731 AlwNI CAG_nnn'CTG Cuts at: 2872 3474 5046 2872 Size: 602 1572 3557 Fragments arranged by size: 3557 1572 602 ApaLI G'TGCA_C Cuts at: 2160 3788 4944 2160 Size: 1628 1156 2947 Fragments arranged by size: 2947 1628 1156 ApoI r'AATT_y Cuts at: 1493 2961 3040 3266 3418 5527 1493 Size: 1468 79 226 152 2109 1697 Fragments arranged by size: 2109 1697 1468 226 152 79 AseI AT'TA_AT Cuts at: 160 1032 4285 160 Size: 872 3253 1606 Fragments arranged by size: 3253 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5731 AvaI C'yCGr_G Cuts at: 1870 1943 2029 2299 3056 1870 Size: 73 86 270 757 4545 Fragments arranged by size: 4545 757 270 86 73 AvrII C'CTAG_G Cuts at: 2998 2998 Size: 5731 BamHI G'GATC_C Cuts at: 3061 3428 3061 Size: 367 5364 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3052 4507 618 Size: 325 217 627 1265 1455 1842 Fragments arranged by size: 1842 1455 1265 627 325 217 BanII G_rGCy'C Cuts at: 1391 2093 2115 2271 2524 2729 3050 1391 Size: 702 22 156 253 205 321 4072 Fragments arranged by size: 4072 702 321 253 205 156 22 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2663 928 Size: 971 764 3996 Fragments arranged by size: 3996 971 764 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2290 2324 2392 2426 3922 3956 1903 Size: 34 353 34 68 34 1496 34 3678 Fragments arranged by size: 3678 1496 353 68 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3623 4833 3623 Size: 1210 4521 BclI T'GATC_A Cuts at: 1548 2638 1548 Size: 1090 4641 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2097 2311 4342 Size: 107 122 71 631 605 425 214 2031 Cuts at: 4342 136 Size: 1525 Fragments arranged by size: 2031 1525 631 605 425 214 122 107 71 BglII A'GATC_T Cuts at: 5726 5726 Size: 5731 BlpI GC'TnA_GC Cuts at: 2844 3148 2844 Size: 304 5427 Bme1580I G_kGCm'C Cuts at: 1790 2077 2164 2260 3792 4948 1790 Size: 287 87 96 1532 1156 2573 Fragments arranged by size: 2573 1532 1156 287 96 87 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5731 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2404 2727 3462 Size: 416 53 83 399 24 987 323 735 Cuts at: 3462 4420 442 Size: 958 1753 Fragments arranged by size: 1753 987 958 735 416 399 323 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5731 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2089 2103 2633 4391 1239 Size: 850 14 530 1758 2579 Fragments arranged by size: 2579 1758 850 530 14 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3856 4721 5019 3856 Size: 865 298 4568 Fragments arranged by size: 4568 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4394 882 Size: 617 2895 2219 Fragments arranged by size: 2895 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3522 493 Size: 711 2318 2702 Fragments arranged by size: 2702 2318 711 BsaBI GATnn'nnATC Cuts at: 5725 5725 Size: 5731 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3538 3920 4555 275 Size: 53 83 186 936 2005 382 635 1451 Fragments arranged by size: 2005 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2142 4164 4562 4836 4983 5394 2142 Size: 2022 398 274 147 411 2479 Fragments arranged by size: 2479 2022 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2938 2968 1178 Size: 30 165 30 1535 30 3941 Fragments arranged by size: 3941 1535 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2047 2757 1186 Size: 861 710 4160 Fragments arranged by size: 4160 861 710 BseYI C'CCAG_C Cuts at: 2100 2545 2916 4934 5430 2100 Size: 445 371 2018 496 2401 Fragments arranged by size: 2401 2018 496 445 371 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2645 1163 Size: 573 909 4249 Fragments arranged by size: 4249 909 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3181 3942 4970 1540 Size: 275 186 1180 761 1028 2301 Fragments arranged by size: 2301 1180 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2115 2164 3792 3877 4948 2115 Size: 49 1628 85 1071 2898 Fragments arranged by size: 2898 1628 1071 85 49 BsmI GAATG_Cn' Cuts at: 3242 3335 3242 Size: 93 5638 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2077 2093 2115 2164 2260 2271 2524 Size: 399 287 16 22 49 96 11 253 Cuts at: 2524 2729 3050 3792 3877 4948 1391 Size: 205 321 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 321 287 253 205 96 85 49 22 16 11 BspEI T'CCGG_A Cuts at: 2142 5394 2142 Size: 3252 2479 BspHI T'CATG_A Cuts at: 3620 5350 3620 Size: 1730 4001 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3086 844 Size: 2242 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3618 1394 Size: 429 1795 3507 Fragments arranged by size: 3507 1795 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2045 2937 4226 4400 65 Size: 1183 797 892 1289 174 1396 Fragments arranged by size: 1396 1289 1183 892 797 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4375 4562 1700 Size: 110 2565 187 2869 Fragments arranged by size: 2869 2565 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5731 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5731 BssSI C'ACGA_G Cuts at: 2411 3791 4803 2411 Size: 1380 1012 3339 Fragments arranged by size: 3339 1380 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3009 794 Size: 2215 3516 BstBI TT'CG_AA Cuts at: 4535 4535 Size: 5731 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5731 BstXI CCAn_nnnn'nTGG Cuts at: 2399 2399 Size: 5731 BstYI r'GATC_y Cuts at: 1972 3061 3428 3813 3830 5282 5368 5380 5726 Size: 1089 367 385 17 1452 86 12 346 Cuts at: 5726 1972 Size: 1977 Fragments arranged by size: 1977 1452 1089 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2498 2838 2925 2498 Size: 340 87 5304 Fragments arranged by size: 5304 340 87 BtgI C'CryG_G Cuts at: 513 1065 2004 2424 513 Size: 552 939 420 3820 Fragments arranged by size: 3820 939 552 420 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4992 Fragments arranged by size: 4992 683 56 BtsI GCAGTG_nn' Cuts at: 2883 3247 4041 4061 2883 Size: 364 794 20 4553 Fragments arranged by size: 4553 794 364 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5731 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5696 DraI TTT'AAA Cuts at: 3035 3035 Size: 5731 DraIII CAC_nnn'GTG Cuts at: 2539 3009 2539 Size: 470 5261 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4738 809 Size: 507 3422 1802 Fragments arranged by size: 3422 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2122 2421 3178 Size: 54 1425 50 275 18 292 299 757 Cuts at: 3178 4067 5696 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 757 299 292 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3178 1537 Size: 275 1366 4090 Fragments arranged by size: 4090 1366 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2187 3661 1353 Size: 834 1474 3423 Fragments arranged by size: 3423 1474 834 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4318 4692 4838 387 Size: 1064 138 2729 374 146 1280 Fragments arranged by size: 2729 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2113 2113 Size: 5731 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2089 2103 2633 3794 4391 5178 5446 Size: 393 457 14 530 1161 597 787 268 Cuts at: 5446 1239 Size: 1524 Fragments arranged by size: 1524 1161 787 597 530 457 393 268 14 EcoO109I rG'GnC_Cy Cuts at: 2128 2221 2744 2128 Size: 93 523 5115 Fragments arranged by size: 5115 523 93 EcoRI G'AATT_C Cuts at: 2961 3040 3418 2961 Size: 79 378 5274 Fragments arranged by size: 5274 378 79 FspI TGC'GCA Cuts at: 4237 4237 Size: 5731 HaeII r_GCGC'y Cuts at: 1678 4878 1678 Size: 3200 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2274 2306 2428 2460 4372 Size: 32 135 32 702 32 122 32 1912 Cuts at: 4372 4404 4446 4478 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1912 702 135 122 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3075 3321 669 Size: 988 281 1137 246 3079 Fragments arranged by size: 3079 1137 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5731 HpaI GTT'AAC Cuts at: 3321 3321 Size: 5731 KpnI G_GTAC'C Cuts at: 3056 3056 Size: 5731 MfeI C'AATT_G Cuts at: 3330 3330 Size: 5731 MluI A'CGCG_T Cuts at: 5607 5607 Size: 5731 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4845 5029 1305 Size: 3540 184 2007 Fragments arranged by size: 3540 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2124 10 Size: 54 1425 343 292 3617 Fragments arranged by size: 3617 1425 343 292 54 MslI CAynn'nnrTG Cuts at: 518 1166 3689 4048 4207 518 Size: 648 2523 359 159 2042 Fragments arranged by size: 2523 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2426 2510 2738 2869 3147 3824 4972 5217 Size: 820 84 228 131 278 677 1148 245 Cuts at: 5217 1606 Size: 2120 Fragments arranged by size: 2120 1148 820 677 278 245 228 131 84 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4240 Fragments arranged by size: 4240 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5731 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5731 NotI GC'GGCC_GC Cuts at: 3178 3178 Size: 5731 NspI r_CATG'y Cuts at: 1132 1229 2407 3089 4634 1132 Size: 97 1178 682 1545 2229 Fragments arranged by size: 2229 1545 1178 682 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5731 PciI A'CATG_T Cuts at: 4630 4630 Size: 5731 PflMI CCAn_nnn'nTGG Cuts at: 1108 2872 1108 Size: 1764 3967 PmeI GTTT'AAAC Cuts at: 3035 3035 Size: 5731 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3800 3832 5313 5345 3800 Size: 32 1481 32 4186 Fragments arranged by size: 4186 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2128 2221 2128 Size: 93 5638 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5731 PsiI TTA'TAA Cuts at: 3301 3301 Size: 5731 PspXI vC'TCGA_Gb Cuts at: 2299 2299 Size: 5731 PstI C_TGCA'G Cuts at: 830 3083 830 Size: 2253 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5731 PvuII CAG'CTG Cuts at: 2510 2869 2510 Size: 359 5372 SacI G_AGCT'C Cuts at: 2115 2115 Size: 5731 SacII CC_GC'GG Cuts at: 2427 2427 Size: 5731 SalI G'TCGA_C Cuts at: 1936 3073 1936 Size: 1137 4594 SapI GCTCTTCn'nnn_ Cuts at: 2187 2187 Size: 5731 SbfI CC_TGCA'GG Cuts at: 3083 3083 Size: 5731 ScaI AGT'ACT Cuts at: 3979 5691 3979 Size: 1712 4019 SfcI C'TryA_G Cuts at: 826 1045 3079 4214 4895 5086 826 Size: 219 2034 1135 681 191 1471 Fragments arranged by size: 2034 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5731 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5731 SmaI CCC'GGG Cuts at: 1872 2031 3058 1872 Size: 159 1027 4545 Fragments arranged by size: 4545 1027 159 SmlI C'TyrA_G Cuts at: 820 1017 1943 2299 3835 4736 4998 820 Size: 197 926 356 1536 901 262 1553 Fragments arranged by size: 1553 1536 926 901 356 262 197 SnaBI TAC'GTA Cuts at: 493 3522 493 Size: 3029 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5731 SphI G_CATG'C Cuts at: 1132 2407 3089 1132 Size: 1275 682 3774 Fragments arranged by size: 3774 1275 682 SspI AAT'ATT Cuts at: 5 52 3655 5 Size: 47 3603 2081 Fragments arranged by size: 3603 2081 47 StuI AGG'CCT Cuts at: 1517 2305 1517 Size: 788 4943 StyI C'CwwG_G Cuts at: 513 1065 2004 2382 2998 3170 513 Size: 552 939 378 616 172 3074 Fragments arranged by size: 3074 939 616 552 378 172 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2179 3758 3775 3928 4113 4558 325 Size: 1255 599 1579 17 153 185 445 1498 Fragments arranged by size: 1579 1498 1255 599 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2235 3977 5689 Size: 274 80 33 51 1443 258 1742 1712 Cuts at: 5689 96 Size: 138 Fragments arranged by size: 1742 1712 1443 274 258 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2938 2970 5691 5723 2938 Size: 32 2721 32 2946 Fragments arranged by size: 2946 2721 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5731 XbaI T'CTAG_A Cuts at: 3067 3067 Size: 5731 XhoI C'TCGA_G Cuts at: 1943 2299 1943 Size: 356 5375 XmaI C'CCGG_G Cuts at: 1870 2029 3056 1870 Size: 159 1027 4545 Fragments arranged by size: 4545 1027 159 XmnI GAAnn'nnTTC Cuts at: 3860 3860 Size: 5731 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3539 276 Size: 53 83 186 936 2005 2468 Fragments arranged by size: 2468 2005 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI