################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FauI FseI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MflI MluI MroI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI StuI SwaI Tsp509I Van91I XbaI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AfaI 471 GTAC GT^AC AgeI 712 ACCGGT A^CCGGT AlwI 640 GATCC GGATC(4/5) AspI 435 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 633 CTCGAG C^[CT]CG[GA]G AviII 521 TGCGCA TGC^GCA BalI 58 TGGCCA TGG^CCA BclI 362 TGATCA T^GATCA BsaWI 712 ACCGGT [AT]^CCGG[AT] BseRI 778 CTCCTC GAGGAG(10/8) BsgI 178 CTGCAC GTGCAG(16/14) BsmAI 127 GAGAC GTCTC(1/5) BsoBI 633 CTCGAG C^[CT]CG[GA]G BsrDI 141 GCAATG GCAATG(2/0) BsrFI 712 ACCGGT [GA]^CCGG[CT] BsrI 341 CCAGT ACTGG(1/-1) Cac8I 843 GCCTGC GC[ACGT]^[ACGT]GC CfoI 522 GCGC GCG^C Cfr10I 712 ACCGGT [GA]^CCGG[CT] DraIII 109 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 58 TGGCCA [CT]^GGCC[GA] EcoT14I 242 CCAAGG C^C[AT][AT]GG FbaI 362 TGATCA T^GATCA FspI 521 TGCGCA TGC^GCA HgaI 610 GACGC GACGC(5/10) HhaI 522 GCGC GCG^C HinP1I 522 GCGC G^CGC MaeII 504 ACGT A^CGT MluNI 58 TGGCCA TGG^CCA MscI 58 TGGCCA TGG^CCA MseI 197 TTAA T^TAA MwoI 449 GCTCCCGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PaeR7I 633 CTCGAG C^TCGAG PflFI 435 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 712 ACCGGT A^CCGGT PleI 71 GAGTC GAGTC(4/5) RsaI 471 GTAC GT^AC SacI 456 GAGCTC GAGCT^C SbfI 844 CCTGCAGG CCTGCA^GG SexAI 110 ACCTGGT A^CC[AT]GGT SmlI 633 CTCGAG C^T[CT][GA]AG Sse8387I 844 CCTGCAGG CCTGCA^GG StyI 242 CCAAGG C^C[AT][AT]GG TaqI 634 TCGA T^CGA TfiI 874 GATTC G^A[AT]TC Tru9I 197 TTAA T^TAA Tth111I 435 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 634 TCGA T^CGA XcmI 341 CCAGTGACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 633 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AflIII 467 ACATGT A^C[GA][CT]GT 732 ACATGT ApaI 405 GGGCCC GGGCC^C 561 GGGCCC Asp700I 80 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 665 GAAACCCTTC BcnI 403 CCGGG CC^[GC]GG 452 CCCGG BspLU11I 467 ACATGT A^CATGT 732 ACATGT BstEII 106 GGTCACC G^GT[ACGT]ACC 715 GGTAACC BstPI 106 GGTCACC G^GT[ACGT]ACC 715 GGTAACC DdeI 393 CTCAG C^T[ACGT]AG 750 CTCAG DpnII 363 GATC ^GATC 640 GATC DsaI 410 CCACGG C^C[GA][CT]GG 573 CCACGG EarI 781 CTCTTC CTCTTC(1/4) 124 GAAGAG EcoO65I 106 GGTCACC G^GT[ACGT]ACC 715 GGTAACC HinfI 71 GAGTC G^A[ACGT]TC 874 GATTC Ksp632I 781 CTCTTC CTCTTC(1/4) 124 GAAGAG MboI 363 GATC ^GATC 640 GATC NciI 403 CCGGG CC^[GC]GG 452 CCCGG NdeII 363 GATC ^GATC 640 GATC NspI 467 ACATGT [GA]CATG^[CT] 732 ACATGT PpuMI 580 GGGACCT [GA]G^G[AT]CC[CT] 745 GGGACCT Sau3AI 363 GATC ^GATC 640 GATC XmnI 80 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 665 GAAACCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AluI AspHI AvaII BanII BbvI BmyI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI Cfr13I DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeIII MboII MnlI MslI MspI MvaI NlaIII NlaIV PstI Sau96I ScrFI SfaNI SfcI TseI Tsp45I TspRI