################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BlnI BsaAI BsaBI BsaHI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoT14I FauI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI StyI SwaI Tth111I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AccII 2317 CGCG CG^CG AccIII 368 TCCGGA T^CCGGA AflIII 1445 ACATGT A^C[GA][CT]GT ApaI 209 GGGCCC GGGCC^C BclI 1080 TGATCA T^GATCA BcnI 1031 CCCGG CC^[GC]GG BpmI 1519 CTGGAG CTGGAG(16/14) BsaI 2042 GAGACC GGTCTC(1/5) BsaWI 368 TCCGGA [AT]^CCGG[AT] BseAI 368 TCCGGA T^CCGGA BsmFI 2631 GTCCC GGGAC(10/14) BspEI 368 TCCGGA T^CCGGA BspHI 1077 TCATGA T^CATGA BspLU11I 1445 ACATGT A^CATGT BsrDI 2622 CATTGC GCAATG(2/0) BstAPI 2496 GCACTCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 2317 CGCG CG^CG DrdI 1037 GACTGGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FbaI 1080 TGATCA T^GATCA HgaI 2034 GCGTC GACGC(5/10) HincII 640 GTCAAC GT[CT]^[GA]AC HindII 640 GTCAAC GT[CT]^[GA]AC MroI 368 TCCGGA T^CCGGA MvnI 2317 CGCG CG^CG NciI 1031 CCCGG CC^[GC]GG PpuMI 1613 AGGACCT [GA]G^G[AT]CC[CT] RcaI 1077 TCATGA T^CATGA SapI 952 GCTCTTC GCTCTTC(1/4) ScaI 1852 AGTACT AGT^ACT SphI 15 GCATGC GCATG^C SspI 1710 AATATT AAT^ATT StuI 905 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AciI 1692 CCGC CCGC(-3/-1) 553 GCGG Asp700I 415 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2146 GAAAACTTTC BalI 845 TGGCCA TGG^CCA 1040 TGGCCA BanII 209 GGGCCC G[GA]GC[CT]^C 1522 GAGCCC BbsI 2118 GAAGAC GAAGAC(2/6) 1331 GTCTTC BlpI 60 GCTGAGC GC^T[ACGT]AGC 2029 GCTGAGC BmyI 209 GGGCCC G[AGT]GC[ACT]^C 1522 GAGCCC Bpu1102I 60 GCTGAGC GC^T[ACGT]AGC 2029 GCTGAGC BpuAI 2118 GAAGAC GAAGAC(2/6) 1331 GTCTTC BseRI 2075 GAGGAG GAGGAG(10/8) 2338 GAGGAG BsmI 324 GAATGC GAATGC(1/-1) 549 GAATGC Bsp1286I 209 GGGCCC G[AGT]GC[ACT]^C 1522 GAGCCC BspMI 144 GCAGGT ACCTGC(4/8) 588 GCAGGT BstXI 1166 CCAAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2126 CCAGGGATGTGG CelII 60 GCTGAGC GC^T[ACGT]AGC 2029 GCTGAGC EaeI 845 TGGCCA [CT]^GGCC[GA] 1040 TGGCCA EarI 953 CTCTTC CTCTTC(1/4) 2470 GAAGAG EcoRI 1964 GAATTC G^AATTC 2584 GAATTC EcoRV 362 GATATC GAT^ATC 508 GATATC HapII 369 CCGG C^CGG 1032 CCGG HpaII 369 CCGG C^CGG 1032 CCGG Ksp632I 953 CTCTTC CTCTTC(1/4) 2470 GAAGAG MluNI 845 TGGCCA TGG^CCA 1040 TGGCCA MscI 845 TGGCCA TGG^CCA 1040 TGGCCA MspI 369 CCGG C^CGG 1032 CCGG NspI 15 GCATGC [GA]CATG^[CT] 1445 ACATGT PleI 1092 GACTC GAGTC(4/5) 1803 GAGTC PstI 586 CTGCAG CTGCA^G 934 CTGCAG XbaI 2196 TCTAGA T^CTAGA 2427 TCTAGA XmnI 415 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2146 GAAAACTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI AvaII BbvI BfaI BglII BsaJI BsgI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT22I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NsiI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII