(Linear) (Six-base) MAPSORT of: KIEE2210.seq Check: 6887 from: 1 to: 2637 KIEE2210.orf With 182 enzymes: SgfI * July 15, 2010 18:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 579 2637 Size: 579 2058 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 853 1625 2227 2467 2637 Size: 853 772 602 240 170 Fragments arranged by size: 853 772 602 240 170 AflIII A'CryG_T Cuts at: 0 1445 2637 Size: 1445 1192 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1678 2637 Size: 1678 959 ApaI G_GGCC'C Cuts at: 0 213 2637 Size: 213 2424 ApoI r'AATT_y Cuts at: 0 265 1483 1558 1964 1984 1993 2512 2584 Size: 265 1218 75 406 20 9 519 72 Cuts at: 2584 2637 Size: 53 Fragments arranged by size: 1218 519 406 265 75 72 53 20 9 BanII G_rGCy'C Cuts at: 0 213 1526 2637 Size: 213 1313 1111 Fragments arranged by size: 1313 1111 213 BbsI GAAGACnn'nnnn_ Cuts at: 0 1324 2125 2637 Size: 1324 801 512 Fragments arranged by size: 1324 801 512 BclI T'GATC_A Cuts at: 0 1080 2637 Size: 1080 1557 BfrBI ATG'CAT Cuts at: 0 19 328 341 1904 2637 Size: 19 309 13 1563 733 Fragments arranged by size: 1563 733 309 19 13 BglII A'GATC_T Cuts at: 0 219 752 879 1411 1493 1940 2637 Size: 219 533 127 532 82 447 697 Fragments arranged by size: 697 533 532 447 219 127 82 BlpI GC'TnA_GC Cuts at: 0 61 2030 2637 Size: 61 1969 607 Fragments arranged by size: 1969 607 61 Bme1580I G_kGCm'C Cuts at: 0 213 2637 Size: 213 2424 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1109 1171 2637 Size: 1109 62 1466 Fragments arranged by size: 1466 1109 62 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1540 2637 Size: 1540 1097 BsaI GGTCTCn'nnnn_ Cuts at: 0 2036 2637 Size: 2036 601 BsaWI w'CCGG_w Cuts at: 0 368 2637 Size: 368 2269 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2069 2099 2637 Size: 2069 30 538 Fragments arranged by size: 2069 538 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 2090 2353 2637 Size: 2090 263 284 Fragments arranged by size: 2090 284 263 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 163 894 1805 2637 Size: 163 731 911 832 Fragments arranged by size: 911 832 731 163 BsmI GAATG_Cn' Cuts at: 0 330 555 2637 Size: 330 225 2082 Fragments arranged by size: 2082 330 225 Bsp1286I G_dGCh'C Cuts at: 0 213 1526 2637 Size: 213 1313 1111 Fragments arranged by size: 1313 1111 213 BspEI T'CCGG_A Cuts at: 0 368 2637 Size: 368 2269 BspHI T'CATG_A Cuts at: 0 1077 2637 Size: 1077 1560 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 135 579 2637 Size: 135 444 2058 Fragments arranged by size: 2058 444 135 BsrDI GCAATG_nn' Cuts at: 0 2621 2637 Size: 2621 16 BstAPI GCAn_nnn'nTGC Cuts at: 0 2502 2637 Size: 2502 135 BstXI CCAn_nnnn'nTGG Cuts at: 0 1173 2133 2637 Size: 1173 960 504 Fragments arranged by size: 1173 960 504 BstYI r'GATC_y Cuts at: 0 219 752 879 1360 1411 1493 1940 2566 Size: 219 533 127 481 51 82 447 626 Cuts at: 2566 2637 Size: 71 Fragments arranged by size: 626 533 481 447 219 127 82 71 51 BtsI GCAGTG_nn' Cuts at: 0 311 943 1660 2637 Size: 311 632 717 977 Fragments arranged by size: 977 717 632 311 DrdI GACnn_nn'nnGTC Cuts at: 0 1043 2637 Size: 1043 1594 EaeI y'GGCC_r Cuts at: 0 845 1040 2637 Size: 845 195 1597 Fragments arranged by size: 1597 845 195 EarI CTCTTCn'nnn_ Cuts at: 0 959 2465 2637 Size: 959 1506 172 Fragments arranged by size: 1506 959 172 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1681 2637 Size: 1681 956 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 853 1540 1625 2227 2467 2637 Size: 853 687 85 602 240 170 Fragments arranged by size: 853 687 602 240 170 85 EcoO109I rG'GnC_Cy Cuts at: 0 210 864 1614 2637 Size: 210 654 750 1023 Fragments arranged by size: 1023 750 654 210 EcoRI G'AATT_C Cuts at: 0 1964 2584 2637 Size: 1964 620 53 Fragments arranged by size: 1964 620 53 EcoRV GAT'ATC Cuts at: 0 364 510 2637 Size: 364 146 2127 Fragments arranged by size: 2127 364 146 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 898 2637 Size: 898 1739 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 739 771 1225 1257 2171 2203 2637 Size: 739 32 454 32 914 32 434 Fragments arranged by size: 914 739 454 434 32 32 32 HincII GTy'rAC Cuts at: 0 642 2637 Size: 642 1995 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1412 1941 2637 Size: 1412 529 696 Fragments arranged by size: 1412 696 529 MscI TGG'CCA Cuts at: 0 847 1042 2637 Size: 847 195 1595 Fragments arranged by size: 1595 847 195 MslI CAynn'nnrTG Cuts at: 0 649 1649 2462 2637 Size: 649 1000 813 175 Fragments arranged by size: 1000 813 649 175 NsiI A_TGCA'T Cuts at: 0 21 330 343 1906 2637 Size: 21 309 13 1563 731 Fragments arranged by size: 1563 731 309 21 13 NspI r_CATG'y Cuts at: 0 19 1449 2637 Size: 19 1430 1188 Fragments arranged by size: 1430 1188 19 PasI CC'CwG_GG Cuts at: 0 2126 2637 Size: 2126 511 PciI A'CATG_T Cuts at: 0 1445 2637 Size: 1445 1192 PfoI T'CCnGG_A Cuts at: 0 2562 2637 Size: 2562 75 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 805 837 2637 Size: 805 32 1800 Fragments arranged by size: 1800 805 32 PpuMI rG'GwC_Cy Cuts at: 0 1614 2637 Size: 1614 1023 PspOMI G'GGCC_C Cuts at: 0 209 2637 Size: 209 2428 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1106 1138 2637 Size: 1106 32 1499 Fragments arranged by size: 1499 1106 32 PstI C_TGCA'G Cuts at: 0 590 938 2637 Size: 590 348 1699 Fragments arranged by size: 1699 590 348 SapI GCTCTTCn'nnn_ Cuts at: 0 959 2637 Size: 959 1678 ScaI AGT'ACT Cuts at: 0 1854 2637 Size: 1854 783 SfcI C'TryA_G Cuts at: 0 586 934 1570 2637 Size: 586 348 636 1067 Fragments arranged by size: 1067 636 586 348 SphI G_CATG'C Cuts at: 0 19 2637 Size: 19 2618 SspI AAT'ATT Cuts at: 0 1712 2637 Size: 1712 925 StuI AGG'CCT Cuts at: 0 907 2637 Size: 907 1730 TatI w'GTAC_w Cuts at: 0 1189 1852 2637 Size: 1189 663 785 Fragments arranged by size: 1189 785 663 XbaI T'CTAG_A Cuts at: 0 2196 2427 2637 Size: 2196 231 210 Fragments arranged by size: 2196 231 210 XmnI GAAnn'nnTTC Cuts at: 0 419 2150 2637 Size: 419 1731 487 Fragments arranged by size: 1731 487 419 Enzymes that do cut: AarI AcuI AflIII AlfI ApaI ApoI BanII BbsI BclI BfrBI BglII BlpI Bme1580I BplI BpmI BsaI BsaWI BsaXI BseRI BsgI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BstAPI BstXI BstYI BtsI DrdI EaeI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I HincII MmeI MscI MslI NsiI NspI PasI PciI PfoI PpiI PpuMI PspOMI PsrI PstI SapI ScaI SfcI SphI SspI StuI TatI XbaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AloI AlwNI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BglI BmgBI BmrI BmtI Bpu10I BpuEI BsaAI BsaBI BsaHI BseYI BsiEI BsiHKAI BsiWI BsmBI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII EagI EcoICRI EcoNI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PflMI PmeI PmlI PshAI PsiI PspXI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI StyI SwaI TaqII TstI Tth111I XcmI XhoI XmaI ZraI